An efficient high-throughput screening assay for rapid directed evolution of halohydrin dehalogenase for preparation of β-substituted alcohols

Applied Microbiology and Biotechnology

Volumn 99, Issue 9, 2015, Pages 4019-4029

  Wan, Nan Wei ^a   Liu, Zhi Qiang ^a   Xue, Feng ^a   Huang, Kai ^a   Tang, Ling Jiao ^a   Zheng, Yu Guo ^a  

^a ZHEJIANG UNIVERSITY OF TECHNOLOGY   (China)

Author keywords

Colorimetric assay; Ethyl (R) 4 cyano 3 hydroxybutanoate; Ethyl (S) 4 chloro 3 hydroxybutanoate; Halohydrin dehalogenase; High throughput screening

Indexed keywords

COLOR; COLORIMETRY;

COLORIMETRIC ASSAYS; ETHYL (R)-4-CYANO-3-HYDROXYBUTANOATE; ETHYL (S)-4-CHLORO-3-HYDROXYBUTANOATE; HALOHYDRIN DEHALOGENASE; HIGH THROUGHPUT SCREENING;

THROUGHPUT;

ALCOHOL DERIVATIVE; ETHYL 4 CHLORO 3 HYDROXYBUTANOATE; ETHYL 4 CYANO 3 HYDROXYBUTANOATE; HALOHYDRIN DEHALOGENASE; OXIDOREDUCTASE; UNCLASSIFIED DRUG; AZIDE; HYDROLASE; MUTANT PROTEIN;

ALCOHOL; BIOASSAY; CATALYSIS; ENZYME ACTIVITY; MUTATION; ORGANIC COMPOUND;

ARTICLE; BACTERIUM; CATALYTIC EFFICIENCY; COLORIMETRY; HIGH THROUGHPUT SCREENING; METHODOLOGY; MOLECULAR DOCKING; MOLECULAR EVOLUTION; NONHUMAN; PARVIBACULUM LAVAMENTIVORANS; RING OPENING; SITE DIRECTED MUTAGENESIS; SUBSTITUTION REACTION; ALPHAPROTEOBACTERIA; AMINO ACID SUBSTITUTION; DIRECTED MOLECULAR EVOLUTION; ENZYMOLOGY; GENETICS; METABOLISM; MISSENSE MUTATION; PROCEDURES;

PARVIBACULUM LAVAMENTIVORANS;

ALCOHOLS; ALPHAPROTEOBACTERIA; AMINO ACID SUBSTITUTION; AZIDES; COLORIMETRY; DIRECTED MOLECULAR EVOLUTION; HIGH-THROUGHPUT SCREENING ASSAYS; HYDROLASES; MUTANT PROTEINS; MUTATION, MISSENSE;

EID: 84938207940     PISSN: 01757598     EISSN: 14320614     Source Type: Journal    
DOI: 10.1007/s00253-015-6527-z     Document Type: Article

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