A high-throughput screening strategy for nitrile-hydrolyzing enzymes based on ferric hydroxamate spectrophotometry

Applied Microbiology and Biotechnology

Volumn 89, Issue 3, 2011, Pages 817-823

  He, Yu Cai ^a,b,d   Ma, Cui Luan ^a,c   Xu, Jian He ^b,d   Zhou, Li ^b  

^a CHANGZHOU UNIVERSITY   (China)

^b EAST CHINA UNIVERSITY OF SCIENCE AND TECHNOLOGY   (China)

^c Changzhou Kangpu Pharmaceutical Co Ltd   (China)

^d HUBEI UNIVERSITY OF TECHNOLOGY   (China)

Author keywords

Carboxylic acids; Evaluation; Ferric hydroxamate spectrophotometry; High throughput screening; Nitrilase

Indexed keywords

ALCALIGENES SP; AROMATIC CARBOXYLIC ACIDS; EVALUATION; HIGH-THROUGHPUT SCREENING; HYDROXAMATE; NITRILASE; PHARMACEUTICAL INDUSTRY; RHODOCOCCUS ERYTHROPOLIS; SCREENING STRATEGY;

CARBOXYLIC ACIDS; CATALYSTS; ENZYMES; HYDROLYSIS; SPECTROPHOTOMETRY; THROUGHPUT;

CYANIDES;

ALIPHATIC CARBOXYLIC ACID; AROMATIC CARBOXYLIC ACID; CARBOXYLIC ACID; FERRIC HYDROXAMATE; HYDROXAMIC ACID DERIVATIVE; NITRILASE; NITRILE; NITRILE AMIDASE; NITRILE HYDRATASE; UNCLASSIFIED DRUG;

BACTERIUM; CARBOXYLIC ACID; DRUG; ENZYME ACTIVITY; HYDROLYSIS; LIQUID CHROMATOGRAPHY; PROTEIN; SPECTROPHOTOMETRY;

ACCURACY; ALCALIGENES; ARTICLE; CONTROLLED STUDY; EVALUATION; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HIGH THROUGHPUT SCREENING; MICROORGANISM; NONHUMAN; PROTEIN ENGINEERING; RHODOCOCCUS ERYTHROPOLIS; ROOM TEMPERATURE; SPECTROPHOTOMETRY;

ALCALIGENES; AMINOHYDROLASES; FERRIC COMPOUNDS; HIGH-THROUGHPUT SCREENING ASSAYS; HYDRO-LYASES; HYDROLYSIS; HYDROXAMIC ACIDS; NITRILES; RHODOCOCCUS; SPECTROPHOTOMETRY;

ALCALIGENES SP.; RHODOCOCCUS ERYTHROPOLIS;

EID: 79251593881     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00253-010-2977-5     Document Type: Article

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