Key residues for controlling enantioselectivity of halohydrin dehalogenase from Arthrobacter sp. strain AD2, revealed by structure-guided directed evolution

Applied and Environmental Microbiology

Volumn 78, Issue 8, 2012, Pages 2631-2637

  Tang, Lixia ^a   Zhu, Xuechen ^a   Zheng, Huayu ^a   Jiang, Rongxiang ^a   Elenkov, Maja Majerić ^b  

^a UNIVERSITY OF ELECTRONIC SCIENCE AND TECHNOLOGY OF CHINA   (China)

^b RUDER BO KOVI INSTITUTE   (Croatia)

Author keywords

[No Author keywords available]

Indexed keywords

AGROBACTERIUM RADIOBACTER AD1; AROMATIC SUBSTRATES; ARTHROBACTER SP; DEHALOGENASE; DESIGN STRATEGIES; DIRECTED EVOLUTION; DOUBLE MUTANTS; HALOHYDRINS; KEY RESIDUES; SATURATION MUTAGENESIS; SPECIFIC ACTIVITY;

AMINO ACIDS; CHIRALITY; ENANTIOMERS; ENZYMES; ETHANOL;

ENANTIOSELECTIVITY;

HALOHYDRIN DEHALOGENASE; HYDROLASE; MUTANT PROTEIN;

ALCOHOL; AMINO ACID; BACTERIUM; CATALYST; ENZYME ACTIVITY; MUTAGENICITY; MUTATION; SUBSTRATE;

AMINO ACID SUBSTITUTION; ARTHROBACTER; ARTICLE; CHEMICAL STRUCTURE; DIRECTED MOLECULAR EVOLUTION; ENZYME ACTIVE SITE; ENZYME SPECIFICITY; ENZYMOLOGY; GENETICS; METABOLISM; PROTEIN TERTIARY STRUCTURE;

AMINO ACID SUBSTITUTION; ARTHROBACTER; CATALYTIC DOMAIN; DIRECTED MOLECULAR EVOLUTION; HYDROLASES; MODELS, MOLECULAR; MUTANT PROTEINS; PROTEIN STRUCTURE, TERTIARY; SUBSTRATE SPECIFICITY;

AGROBACTERIUM TUMEFACIENS; ARTHROBACTER SP.;

EID: 84861163924     PISSN: 00992240     EISSN: 10985336     Source Type: Journal    
DOI: 10.1128/AEM.06586-11     Document Type: Article

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