Engineering a nickase on the homing endonuclease I-DmoI Scaffold

Journal of Biological Chemistry

Volumn 290, Issue 30, 2015, Pages 18534-18544

  Molina, Rafael ^a   Marcaida, María José ^a,e   Redondo, Pilar ^a   Marenchino, Marco ^a   Duchateau, Phillippe ^b   D'Abramo, Marco ^c   Montoya, Guillermo ^a,d   Prieto, Jesús ^a  

^a Structural Biology and Biocomputing Programme   (Spain)

^b Arvid Wallgrens Backe 20   (France)

^c SAPIENZA UNIVERSITY OF ROME   (Italy)

^d University of Copenhagen   (Denmark)

^e UNIVERSITY OF BERN   (Switzerland)

Author keywords

[No Author keywords available]

Indexed keywords

JOINING; MACHINERY; SCAFFOLDS;

BIOCHEMICAL ANALYSIS; DNA DAMAGE RESPONSE; DNA DOUBLE STRAND BREAKS; DOUBLE STRAND BREAKS; GENOME MODIFICATION; HOMOLOGOUS RECOMBINATION; NONHOMOLOGOUS END JOINING; SINGLE-STRAND BREAKS;

DNA;

DEOXYRIBONUCLEASE I; METAL; DNA BINDING PROTEIN; ENDODEOXYRIBONUCLEASE I-DMO I; TYPE I SITE SPECIFIC DEOXYRIBONUCLEASE;

ARTICLE; BINDING AFFINITY; CATALYSIS; CONTROLLED STUDY; CRYSTAL STRUCTURE; DNA CLEAVAGE; DNA NICK TRANSLATION; DNA STRAND; DOUBLE STRANDED DNA BREAK; METAL BINDING; MOLECULAR DYNAMICS; MUTATION; NUCLEOPHILICITY; PRIORITY JOURNAL; PROTEIN FUNCTION; PROTEIN HYDROLYSIS; PROTEIN STABILITY; PROTON TRANSPORT; CHEMISTRY; CIRCULAR DICHROISM; DNA END JOINING REPAIR; ENZYME ACTIVE SITE; GENE TARGETING; GENETICS; HOMOLOGOUS RECOMBINATION; METABOLISM; PROTEIN ENGINEERING; PROTEIN MOTIF; X RAY CRYSTALLOGRAPHY;

AMINO ACID MOTIFS; CATALYTIC DOMAIN; CIRCULAR DICHROISM; CRYSTALLOGRAPHY, X-RAY; DEOXYRIBONUCLEASE I; DEOXYRIBONUCLEASES, TYPE I SITE-SPECIFIC; DNA BREAKS, DOUBLE-STRANDED; DNA END-JOINING REPAIR; DNA-BINDING PROTEINS; GENE TARGETING; HOMOLOGOUS RECOMBINATION; MOLECULAR DYNAMICS SIMULATION; PROTEIN ENGINEERING;

EID: 84937706331     PISSN: 00219258     EISSN: 1083351X     Source Type: Journal    
DOI: 10.1074/jbc.M115.658666     Document Type: Article

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