Engineering Variants of the I-SceI Homing Endonuclease with Strand-specific and Site-specific DNA-nicking Activity

Journal of Molecular Biology

Volumn 382, Issue 1, 2008, Pages 188-202

  Niu, Yan ^a   Tenney, Kristen ^a   Li, Hongye ^b   Gimble, Frederick S ^a  

^a PURDUE UNIVERSITY   (United States)

^b TEXAS A AND M HEALTH SCIENCE CENTER   (United States)

Author keywords

active site mutagenesis; enzyme kinetics; homing endonuclease; protein engineering; strand specific endonuclease

Indexed keywords

ENDONUCLEASE; HOMING ENDONUCLEASE I SCEL; LYSINE;

AMINO ACID SUBSTITUTION; ARTICLE; CHEMICAL BOND; CRYSTAL STRUCTURE; DNA CLEAVAGE; DNA PROTEIN COMPLEX; DNA SEQUENCE; DNA STRAND; DNA SUPERCOILING; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME ENGINEERING; ENZYME KINETICS; ENZYME MECHANISM; ENZYME SPECIFICITY; ENZYME STRUCTURE; ENZYME SUBSTRATE; GENE TARGETING; MOLECULAR RECOGNITION; PRIORITY JOURNAL; PROTEIN DNA INTERACTION;

EID: 49349086561     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jmb.2008.07.010     Document Type: Article

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