A single-chain variable fragment intrabody prevents intracellular polymerization of Z α1-antitrypsin while allowing its antiproteinase activity

FASEB Journal

Volumn 29, Issue 6, 2015, Pages 2667-2678

  Ordóñez, Adriana ^a   Pérez, Juan ^b   Tan, Lu ^a   Dickens, Jennifer A ^a   Motamedi Shad, Neda ^c   Irving, James A ^c   Haq, Imran ^c   Ekeowa, Ugo ^a   Marciniak, Stefan J ^a   Miranda, Elena ^d   Lomas, David A ^c  

^a UNIVERSITY OF CAMBRIDGE   (United Kingdom)

^b UNIVERSITY OF MÁLAGA   (Spain)

^c UNIVERSITY COLLEGE LONDON   (United Kingdom)

^d SAPIENZA UNIVERSITY OF ROME   (Italy)

Author keywords

Liver disease; Monoclonal antibody; ScFv intrabody; Serpin polymer

Indexed keywords

ALPHA 1 ANTITRYPSIN; ALPHA 1 ANTITRYPSIN TYPE Z; COMPLEMENTARY DNA; GLUCOSE REGULATED PROTEIN 78; GLUCOSE REGULATED PROTEIN 94; LEUKOCYTE ELASTASE; MONOCLONAL ANTIBODY; MONOCLONAL ANTIBODY 4B12; MONOCLONAL ANTIBODY 9C5; PROTEINASE INHIBITOR; SINGLE CHAIN FRAGMENT VARIABLE ANTIBODY; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; ANIMAL CELL; ANIMAL EXPERIMENT; ANTIGEN BINDING; ARTICLE; CELL FUSION; CELL INCLUSION; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; CRYSTAL STRUCTURE; DNA SEQUENCE; DRUG SCREENING; ENDOPLASMIC RETICULUM STRESS; ENZYME LINKED IMMUNOSORBENT ASSAY; ENZYME REGULATION; GENETIC TRANSFECTION; HUMAN; IN VITRO STUDY; MOLECULAR DYNAMICS; MOLECULAR WEIGHT; MOUSE; NONHUMAN; POLYMERIZATION; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN EXPRESSION; PROTEIN INTERACTION; PROTEIN SECRETION; PROTEINASE INHIBITION; SECRETORY PATHWAY; STEADY STATE; STOICHIOMETRY; ANIMAL; BAGG ALBINO MOUSE; CHLOROCEBUS AETHIOPS; COS 1 CELL LINE; DRUG EFFECTS; ENDOPLASMIC RETICULUM; GENETICS; IMMUNOBLOTTING; IMMUNOLOGY; INTRACELLULAR SPACE; KINETICS; METABOLISM; MOLECULAR GENETICS; MUTATION; NUCLEOTIDE SEQUENCE;

ALPHA 1-ANTITRYPSIN; ANIMALS; BASE SEQUENCE; CERCOPITHECUS AETHIOPS; COS CELLS; ENDOPLASMIC RETICULUM; HUMANS; IMMUNOBLOTTING; INTRACELLULAR SPACE; KINETICS; LEUKOCYTE ELASTASE; MICE, INBRED BALB C; MOLECULAR SEQUENCE DATA; MUTATION; POLYMERIZATION; PROTEASE INHIBITORS; SINGLE-CHAIN ANTIBODIES;

EID: 84933567270     PISSN: 08926638     EISSN: 15306860     Source Type: Journal    
DOI: 10.1096/fj.14-267351     Document Type: Article

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