A simplified and robust protocol for immunoglobulin expression in Escherichia coli cell-free protein synthesis systems

Biotechnology Progress

Volumn 31, Issue 3, 2015, Pages 823-831

  Cai, Qi ^a   Hanson, Jeffrey A ^a   Steiner, Alexander R ^a   Tran, Cuong ^a   Masikat, Mary Rose ^a   Chen, Rishard ^a   Zawada, James F ^a   Sato, Aaron K ^a   Hallam, Trevor J ^a   Yin, Gang ^a  

^a Sutro Biopharma Inc ^*  (United States)

Author keywords

Cell free; Expression; IgG; Immunoglobulin G; Protein synthesis

Indexed keywords

CELLS; COENZYMES; COST REDUCTION; CYTOLOGY; ESCHERICHIA COLI; MIXTURES; MOLECULAR BIOLOGY; MONOCLONAL ANTIBODIES; OPERATING COSTS; PRODUCTIVITY; REAGENTS; SENSITIVITY ANALYSIS;

CELL FREE PROTEIN SYNTHESIS; CELL-FREE; EXPRESSION; EXPRESSION IN ESCHERICHIA COLI; HIGH THROUGHPUT SCREENING; IMMUNOGLOBULIN G; PROTEIN SYNTHESIS; TECHNOLOGICAL DEVELOPMENT;

BIOSYNTHESIS;

ESCHERICHIA COLI;

BACTERIOPHAGE T7 RNA POLYMERASE; COENZYME A; DNA DIRECTED RNA POLYMERASE; FOLINIC ACID; GLUTAMIC ACID; IMMUNOGLOBULIN G; NICOTINAMIDE ADENINE DINUCLEOTIDE; POLYAMINE; PUTRESCINE; PYRUVIC ACID; TRANSFER RNA; TRASTUZUMAB; VIRUS PROTEIN;

BIOSYNTHESIS; CHEMISTRY; ESCHERICHIA COLI; GENE EXPRESSION; GENETICS; METABOLISM; PROCEDURES; PROTEIN ENGINEERING; PROTEIN FOLDING; PROTEIN SYNTHESIS; REPRODUCIBILITY;

COENZYME A; DNA-DIRECTED RNA POLYMERASES; ESCHERICHIA COLI; GENE EXPRESSION; GLUTAMIC ACID; IMMUNOGLOBULIN G; LEUCOVORIN; NAD; POLYAMINES; PROTEIN BIOSYNTHESIS; PROTEIN ENGINEERING; PROTEIN FOLDING; PUTRESCINE; PYRUVIC ACID; REPRODUCIBILITY OF RESULTS; RNA, TRANSFER; TRASTUZUMAB; VIRAL PROTEINS;

EID: 84931564680     PISSN: 87567938     EISSN: 15206033     Source Type: Journal    
DOI: 10.1002/btpr.2082     Document Type: Article

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