Site-specific incorporation of unnatural amino acids into proteins by cell-free protein synthesis

Methods in Molecular Biology

Volumn 1118, Issue , 2014, Pages 189-203

  Ozawa, Kiyoshi ^a   Loh, Choy Theng ^b  

^a UNIVERSITY OF WOLLONGONG   (Australia)

^b AUSTRALIAN NATIONAL UNIVERSITY   (Australia)

Author keywords

Aminoacyl tRNA synthetase; Cell free protein synthesis; Copper free click chemistry; Suppressor tRNA; Unnatural amino acids

Indexed keywords

AMINO ACID DERIVATIVE; AMINO ACID TRANSFER RNA LIGASE; ESCHERICHIA COLI PROTEIN; TRANSFER RNA;

ARTICLE; CELL FREE PROTEIN SYNTHESIS; CELL FREE SYSTEM; CLICK CHEMISTRY; CYCLOADDITION; ESCHERICHIA COLI; METHANOCALDOCOCCUS JANNASCHII; MOLECULAR PROBE; NONHUMAN; PRIORITY JOURNAL; PROTEIN ENGINEERING; PROTEIN EXPRESSION; PROTEIN PROCESSING; PROTEIN SYNTHESIS; STOP CODON;

AMINO ACIDS; AMINO ACYL-TRNA SYNTHETASES; AZIDES; BINDING SITES; CELL EXTRACTS; CELL-FREE SYSTEM; CLICK CHEMISTRY; ESCHERICHIA COLI; FLUORESCENT DYES; METHANOCALDOCOCCUS; PHENYLALANINE; PROTEIN BIOSYNTHESIS; PROTEIN ENGINEERING; RNA, TRANSFER; SUPPRESSION, GENETIC;

EID: 84934441987     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-62703-782-2_12     Document Type: Article

References (25)

1. Kigawa T, Yabuki T, Yoshida Y et al (1999) Cell-free production and stable-isotope labeling of milligram quantities of proteins. FEBS Lett 442:15-19 (Pubitemid 29065369)
2. Renesto P, Raoult D (2003) In vitro expression of rickettsial proteins. Ann N Y Acad Sci 990: 642-645
3. Kigawa T, Yamaguchi-Nunokawa E, Kodama K et al (2001) Selenomethionine incorporation into a protein by cell-free synthesis. J Struct Funct Genomics 2:29-35
4. Ozawa K, Headlam MJ, Mouradov D et al (2005) Translational incorporation of L-3,4-dihydroxyphenylalanine into proteins. FEBS J 272:3162-3171 (Pubitemid 40904701)
5. Ozawa K, Jergic S, Crowther JA et al (2005) Cell-free protein synthesis in an autoinduction system for NMR studies of protein-protein interactions. J Biomol NMR 32:235-241 (Pubitemid 41258803)
6. Ozawa K, Jergic S, Park AY et al (2008) The proofreading exonuclease subunit of Escherichia coli DNA polymerase III is tethered to the polymerase subunit via a fl exible linker. Nucleic Acids Res 36:5074-5082
7. Klammt C, Löhr F, Schäfer B et al (2004) High level cell-free expression and specifi c labeling of integral membrane proteins. Eur J Biochem 271:568-580 (Pubitemid 38183631)
8. Klammt C, Schwarz D, Löhr F et al (2006) Cell-free expression as an emerging technique for the large scale production of integral membrane protein. FEBS J 273:4141-4153 (Pubitemid 44326495)
9. Ozawa K, Dixon NE, Otting G (2005) Cellfree synthesis of 15 N-labeled proteins for NMR studies. IUBMB Life 57:615-622 (Pubitemid 41530549)
10. Ozawa K, Wu PSC, Dixon NE et al (2006) 15 N-Labelled proteins by cell-free protein synthesis: strategies for high-throughput NMR studies of proteins and protein-ligand complexes. FEBS J 273:4154-4159
11. Shimizu Y, Kuruma Y, Ying BW et al (2006) Cell-free translation systems for protein engineering. FEBS J 273:4133-4140 (Pubitemid 44326494)
12. Wang L, Schultz PG (2001) A general approach for the generation of orthogonal tRNAs. Chem Biol 8:883-890 (Pubitemid 32863205)
13. Liu CC, Schultz PG (2010) Adding new chemistries to the genetic code. Annu Rev Biochem 79:413-444
14. Young TS, Ahmad I, Yin JA et al (2010) An enhanced system for unnatural amino acid mutagenesis in E. coli . J Mol Biol 395: 361-374
15. Ozawa K, Loscha KV, Kuppan KV et al (2012) High-yield cell-free protein synthesis for sitespecifi c incorporation of unnatural amino acids at two sites. Biochem Biophys Res Commun 418:652-656
16. Neylon C, Brown SE, Kralicek AV et al (2000) Interaction of the Escherichia coli replication terminator protein (Tus) with DNA: a model derived from DNA-binding studies of mutant proteins by surface plasmon resonance. Biochemistry 39:11989-11999
17. Wu PSC, Ozawa K, Lim SP et al (2007) Cellfree transcription/translation from PCR amplifi ed DNA for high-throughput NMR studies. Angew Chem Int Ed 46:3356-3358 (Pubitemid 46973496)
18. Fleissner MR, Brustad EM, Kálai T et al (2009) Site-directed spin labeling of a genetically encoded unnatural amino acid. Proc Natl Acad Sci U S A 106:21637-21642
19. Hong V, Presolski SI, Ma C et al (2009) Analysis and optimization of copper-catalyzed azide-alkyne cycloaddition for bioconjugation. Angew Chem Int Ed 48:9879-9883
20. Pratt JM (1984) Coupled transcription-translation in prokaryotic cell-free systems. In: Hames BD, Higgins SJ (eds) Transcription and translation. IRL Press, Oxford, pp 179-209
21. Apponyi M, Ozawa K, Dixon NE et al (2008) Cell-free protein synthesis for analysis by NMR spectroscopy. In: Kobe B, Guss M, Huber T (eds) Methods in molecular biology. Structural proteomics: high-throughput methods, vol 426. Humana, Totowa, NJ, pp 257-268
22. Jergic S, Ozawa K, Williams NK et al (2007) The unstructured C-terminus of the subunit of Escherichia coli DNA polymerase III holoenzyme is the site of interaction with the subunit. Nucleic Acids Res 35:2813-2824 (Pubitemid 47073621)
23. Loscha KV, Herlt AJ, Qi R, Huber T et al (2012) Multiple-site labeling of proteins with unnatural amino acids. Angew Chem Int Ed 51:2243-2246
24. Guo J, Melançon CE 3rd, Lee HS et al (2009) Evolution of amber suppressor tRNAs for effi-cient bacterial production of proteins containing nonnatural amino acids. Angew Chem Int Ed Engl 48(48):9148-9151
25. Chin JW, Santoro SW, Martin AB et al (2002) Addition of p-azido-L-phenylalanine to the genetic code of Escherichia coli . J Am Chem Soc 124(31):9026-9027 (Pubitemid 34847644)