메뉴 건너뛰기




Volumn 35, Issue 2, 2013, Pages 143-152

Cell-free protein synthesis: The state of the art

Author keywords

Cell free translation; Protein engineering; Protein synthesis; PURE expression; Synthetic biology

Indexed keywords

BIOCHEMICAL ENGINEERING; BIOCHIPS; CELL ENGINEERING; CELLS; CYTOLOGY; GENETIC ENGINEERING; MACHINERY; POLYMERASE CHAIN REACTION; RECOMBINANT PROTEINS; SYNTHETIC BIOLOGY;

EID: 84872807174     PISSN: 01415492     EISSN: 15736776     Source Type: Journal    
DOI: 10.1007/s10529-012-1075-4     Document Type: Review
Times cited : (42)

References (69)
  • 1
    • 77951903021 scopus 로고    scopus 로고
    • Membrane protein assembly into Nanodiscs
    • Bayburt TH, Sligar SG (2010) Membrane protein assembly into Nanodiscs. FEBS Lett 584: 1721-1727.
    • (2010) FEBS Lett , vol.584 , pp. 1721-1727
    • Bayburt, T.H.1    Sligar, S.G.2
  • 2
    • 79952599664 scopus 로고    scopus 로고
    • Synthesis of RNA by in vitro transcription
    • Beckert B, Masquida B (2011) Synthesis of RNA by in vitro transcription. Methods Mol Biol 703: 29-41.
    • (2011) Methods Mol Biol , vol.703 , pp. 29-41
    • Beckert, B.1    Masquida, B.2
  • 3
    • 79959913133 scopus 로고    scopus 로고
    • Protein microarrays: novel developments and applications
    • Berrade L, Garcia AE, Camarero JA (2011) Protein microarrays: novel developments and applications. Pharm Res 28: 1480-1499.
    • (2011) Pharm Res , vol.28 , pp. 1480-1499
    • Berrade, L.1    Garcia, A.E.2    Camarero, J.A.3
  • 4
    • 0000640710 scopus 로고    scopus 로고
    • Chapter 5. 2. 3, Modulation of chemical composition and other parameters of the cell at different exponential growth rates
    • In: Böck A, Curtiss R III, Kaper JB, Karp PD, Neidhardt FC, Nyström T, Slauch JM, Squires CL, Ussery D (eds), ASM Press, Washington, DC
    • Bremer H, Dennis PP (2008) Chapter 5. 2. 3, Modulation of chemical composition and other parameters of the cell at different exponential growth rates. In: Böck A, Curtiss R III, Kaper JB, Karp PD, Neidhardt FC, Nyström T, Slauch JM, Squires CL, Ussery D (eds) EcoSal-Escherichia coli and Salmonella: Cellular and molecular biology. http://www. ecosal. org. ASM Press, Washington, DC.
    • (2008) EcoSal-Escherichia coli and Salmonella: Cellular and molecular biology
    • Bremer, H.1    Dennis, P.P.2
  • 5
    • 77049113819 scopus 로고    scopus 로고
    • Site-specific incorporation of p-propargyloxyphenylalanine in a cell-free environment for direct protein-protein click conjugation
    • Bundy BC, Swartz JR (2010) Site-specific incorporation of p-propargyloxyphenylalanine in a cell-free environment for direct protein-protein click conjugation. Bioconjug Chem 21: 255-263.
    • (2010) Bioconjug Chem , vol.21 , pp. 255-263
    • Bundy, B.C.1    Swartz, J.R.2
  • 6
    • 34547699646 scopus 로고    scopus 로고
    • Energy systems for ATP regeneration in cell-free protein synthesis reactions
    • Calhoun KA, Swartz JR (2007) Energy systems for ATP regeneration in cell-free protein synthesis reactions. Methods Mol Biol 375: 3-17.
    • (2007) Methods Mol Biol , vol.375 , pp. 3-17
    • Calhoun, K.A.1    Swartz, J.R.2
  • 9
    • 36448968253 scopus 로고    scopus 로고
    • Elongation factor Tu mutants expand amino acid tolerance of protein biosynthesis system
    • Doi Y, Ohtsuki T, Shimizu Y, Ueda T, Sisido M (2007) Elongation factor Tu mutants expand amino acid tolerance of protein biosynthesis system. J Am Chem Soc 129: 14458-14462.
    • (2007) J Am Chem Soc , vol.129 , pp. 14458-14462
    • Doi, Y.1    Ohtsuki, T.2    Shimizu, Y.3    Ueda, T.4    Sisido, M.5
  • 10
    • 70350491151 scopus 로고    scopus 로고
    • Engineering multigene expression in vitro and in vivo with small terminators for T7 RNA polymerase
    • Du L, Gao R, Forster AC (2009) Engineering multigene expression in vitro and in vivo with small terminators for T7 RNA polymerase. Biotechnol Bioeng 104: 1189-1196.
    • (2009) Biotechnol Bioeng , vol.104 , pp. 1189-1196
    • Du, L.1    Gao, R.2    Forster, A.C.3
  • 11
    • 59649098288 scopus 로고    scopus 로고
    • A single-step method for purification of active His-tagged ribosomes from a genetically engineered Escherichia coli
    • Ederth J, Mandava CS, Dasgupta S, Sanyal S (2009) A single-step method for purification of active His-tagged ribosomes from a genetically engineered Escherichia coli. Nucleic Acids Res 37: e15.
    • (2009) Nucleic Acids Res , vol.37
    • Ederth, J.1    Mandava, C.S.2    Dasgupta, S.3    Sanyal, S.4
  • 12
    • 82955217263 scopus 로고    scopus 로고
    • Cell-free protein expression under macromolecular crowding conditions
    • Ge X, Luo D, Xu J (2011) Cell-free protein expression under macromolecular crowding conditions. PLoS One 6: e28707.
    • (2011) PLoS One , vol.6
    • Ge, X.1    Luo, D.2    Xu, J.3
  • 13
    • 38449099301 scopus 로고    scopus 로고
    • Development of cell-free protein synthesis platforms for disulfide bonded proteins
    • Goerke AR, Swartz JR (2008) Development of cell-free protein synthesis platforms for disulfide bonded proteins. Biotechnol Bioeng 99: 351-367.
    • (2008) Biotechnol Bioeng , vol.99 , pp. 351-367
    • Goerke, A.R.1    Swartz, J.R.2
  • 14
    • 58249085422 scopus 로고    scopus 로고
    • High-level cell-free synthesis yields of proteins containing site-specific non-natural amino acids
    • Goerke AR, Swartz JR (2009) High-level cell-free synthesis yields of proteins containing site-specific non-natural amino acids. Biotechnol Bioeng 102: 400-416.
    • (2009) Biotechnol Bioeng , vol.102 , pp. 400-416
    • Goerke, A.R.1    Swartz, J.R.2
  • 15
    • 84858216324 scopus 로고    scopus 로고
    • Flexizymes as a tRNA acylation tool facilitating genetic code reprogramming
    • Goto Y, Suga H (2012) Flexizymes as a tRNA acylation tool facilitating genetic code reprogramming. Methods Mol Biol 848: 465-478.
    • (2012) Methods Mol Biol , vol.848 , pp. 465-478
    • Goto, Y.1    Suga, H.2
  • 16
    • 79958158209 scopus 로고    scopus 로고
    • Flexizymes for genetic code reprogramming
    • Goto Y, Katoh T, Suga H (2011) Flexizymes for genetic code reprogramming. Nat Protoc 6: 779-790.
    • (2011) Nat Protoc , vol.6 , pp. 779-790
    • Goto, Y.1    Katoh, T.2    Suga, H.3
  • 17
    • 43049128964 scopus 로고    scopus 로고
    • An expanded set of amino acid analogs for the ribosomal translation of unnatural peptides
    • Hartman MC, Josephson K, Lin CW, Szostak JW (2007) An expanded set of amino acid analogs for the ribosomal translation of unnatural peptides. PLoS One 2: e972.
    • (2007) PLoS One , vol.2
    • Hartman, M.C.1    Josephson, K.2    Lin, C.W.3    Szostak, J.W.4
  • 18
    • 83255164998 scopus 로고    scopus 로고
    • Cell-free synthetic biology: thinking outside the cell
    • Hodgman CE, Jewett MC (2012) Cell-free synthetic biology: thinking outside the cell. Metab Eng 14: 261-269.
    • (2012) Metab Eng , vol.14 , pp. 261-269
    • Hodgman, C.E.1    Jewett, M.C.2
  • 19
    • 1542720448 scopus 로고    scopus 로고
    • Mimicking the Escherichia coli cytoplasmic environment activates long-lived and efficient cell-free protein synthesis
    • Jewett MC, Swartz JR (2004) Mimicking the Escherichia coli cytoplasmic environment activates long-lived and efficient cell-free protein synthesis. Biotechnol Bioeng 86: 19-26.
    • (2004) Biotechnol Bioeng , vol.86 , pp. 19-26
    • Jewett, M.C.1    Swartz, J.R.2
  • 20
    • 53949093950 scopus 로고    scopus 로고
    • An integrated cell-free metabolic platform for protein production and synthetic biology
    • Jewett MC, Calhoun KA, Voloshin A, Wuu JJ, Swartz JR (2008) An integrated cell-free metabolic platform for protein production and synthetic biology. Mol Syst Biol 4: 220.
    • (2008) Mol Syst Biol , vol.4 , pp. 220
    • Jewett, M.C.1    Calhoun, K.A.2    Voloshin, A.3    Wuu, J.J.4    Swartz, J.R.5
  • 21
    • 44449089912 scopus 로고    scopus 로고
    • The kinetics of ribosomal peptidyl transfer revisited
    • Johansson M, Bouakaz E, Lovmar M, Ehrenberg M (2008) The kinetics of ribosomal peptidyl transfer revisited. Mol Cell 30: 589-598.
    • (2008) Mol Cell , vol.30 , pp. 589-598
    • Johansson, M.1    Bouakaz, E.2    Lovmar, M.3    Ehrenberg, M.4
  • 24
    • 34447630299 scopus 로고    scopus 로고
    • Functional cell-free synthesis of a seven helix membrane protein: in situ insertion of bacteriorhodopsin into liposomes
    • Kalmbach R, Chizhov I, Schumacher MC, Friedrich T, Bamberg E, Engelhard M (2007) Functional cell-free synthesis of a seven helix membrane protein: in situ insertion of bacteriorhodopsin into liposomes. Mol Biol 371: 639-648.
    • (2007) Mol Biol , vol.371 , pp. 639-648
    • Kalmbach, R.1    Chizhov, I.2    Schumacher, M.C.3    Friedrich, T.4    Bamberg, E.5    Engelhard, M.6
  • 27
    • 67649400884 scopus 로고    scopus 로고
    • Methods for energizing cell-free protein synthesis
    • Kim HC, Kim DM (2009) Methods for energizing cell-free protein synthesis. J Biosci Bioeng 108: 1-4.
    • (2009) J Biosci Bioeng , vol.108 , pp. 1-4
    • Kim, H.C.1    Kim, D.M.2
  • 28
    • 34547623256 scopus 로고    scopus 로고
    • Prolonged cell-free protein synthesis using dual energy sources: combined use of creatine phosphate and glucose for the efficient supply of ATP and retarded accumulation of phosphate
    • Kim TW, Oh IS, Keum JW, Kwon YC, Byun JY, Lee KH, Choi CY, Kim DM (2007) Prolonged cell-free protein synthesis using dual energy sources: combined use of creatine phosphate and glucose for the efficient supply of ATP and retarded accumulation of phosphate. Biotechnol Bioeng 96: 1510-1515.
    • (2007) Biotechnol Bioeng , vol.96 , pp. 1510-1515
    • Kim, T.W.1    Oh, I.S.2    Keum, J.W.3    Kwon, Y.C.4    Byun, J.Y.5    Lee, K.H.6    Choi, C.Y.7    Kim, D.M.8
  • 29
    • 50249159403 scopus 로고    scopus 로고
    • Continuous cell-free protein synthesis using glycolytic intermediates as energy sources
    • Kim HC, Kim TW, Park CG, Oh IS, Park K, Kim DM (2008) Continuous cell-free protein synthesis using glycolytic intermediates as energy sources. J Microbiol Biotechnol 18: 885-888.
    • (2008) J Microbiol Biotechnol , vol.18 , pp. 885-888
    • Kim, H.C.1    Kim, T.W.2    Park, C.G.3    Oh, I.S.4    Park, K.5    Kim, D.M.6
  • 30
    • 79955591560 scopus 로고    scopus 로고
    • Prolonged production of proteins in a cell-free protein synthesis system using polymeric carbohydrates as an energy source
    • Kim HC, Kim TW, Kim DM (2011) Prolonged production of proteins in a cell-free protein synthesis system using polymeric carbohydrates as an energy source. Process Biochem 46: 1366-1369.
    • (2011) Process Biochem , vol.46 , pp. 1366-1369
    • Kim, H.C.1    Kim, T.W.2    Kim, D.M.3
  • 31
    • 34447324367 scopus 로고    scopus 로고
    • Cell-free synthesis of proteins that require disulfide bonds using glucose as an energy source
    • Knapp KG, Goerke AR, Swartz JR (2007) Cell-free synthesis of proteins that require disulfide bonds using glucose as an energy source. Biotechnol Bioeng 97: 901-908.
    • (2007) Biotechnol Bioeng , vol.97 , pp. 901-908
    • Knapp, K.G.1    Goerke, A.R.2    Swartz, J.R.3
  • 33
    • 84859043744 scopus 로고    scopus 로고
    • Flexible programming of cell-free protein synthesis using magnetic bead-immobilized plasmids
    • Lee KY, Lee KH, Park JW, Kim DM (2012) Flexible programming of cell-free protein synthesis using magnetic bead-immobilized plasmids. PLoS One 7: e34429.
    • (2012) PLoS One , vol.7
    • Lee, K.Y.1    Lee, K.H.2    Park, J.W.3    Kim, D.M.4
  • 34
    • 77952481131 scopus 로고    scopus 로고
    • Cardiac titin: a multifunctional giant
    • LeWinter MM, Granzier H (2010) Cardiac titin: a multifunctional giant. Circulation 121: 2137-2145.
    • (2010) Circulation , vol.121 , pp. 2137-2145
    • LeWinter, M.M.1    Granzier, H.2
  • 35
    • 70349545940 scopus 로고    scopus 로고
    • Aminoacyl-tRNA synthesis and translational quality control
    • Ling J, Reynolds N, Ibba M (2009) Aminoacyl-tRNA synthesis and translational quality control. Annu Rev Microbiol 63: 61-78.
    • (2009) Annu Rev Microbiol , vol.63 , pp. 61-78
    • Ling, J.1    Reynolds, N.2    Ibba, M.3
  • 36
    • 77953643054 scopus 로고    scopus 로고
    • Adding new chemistries to the genetic code
    • Liu CC, Schultz PG (2010) Adding new chemistries to the genetic code. Annu Rev Biochem 79: 413-444.
    • (2010) Annu Rev Biochem , vol.79 , pp. 413-444
    • Liu, C.C.1    Schultz, P.G.2
  • 40
    • 0030670682 scopus 로고    scopus 로고
    • Concept and early development of solid-phase peptide synthesis
    • Merrifield B (1997) Concept and early development of solid-phase peptide synthesis. Methods Enzymol 289: 3-13.
    • (1997) Methods Enzymol , vol.289 , pp. 3-13
    • Merrifield, B.1
  • 41
    • 54049139149 scopus 로고    scopus 로고
    • A human cell-derived in vitro coupled transcription/translation system optimized for production of recombinant proteins
    • Mikami S, Kobayashi T, Masutani M, Yokoyama S, Imataka H (2008) A human cell-derived in vitro coupled transcription/translation system optimized for production of recombinant proteins. Protein Expr Purif 62: 190-198.
    • (2008) Protein Expr Purif , vol.62 , pp. 190-198
    • Mikami, S.1    Kobayashi, T.2    Masutani, M.3    Yokoyama, S.4    Imataka, H.5
  • 42
    • 68449097384 scopus 로고    scopus 로고
    • Species-independent translational leaders facilitate cell-free expression
    • Mureev S, Kovtun O, Nguyen UT, Alexandrov K (2009) Species-independent translational leaders facilitate cell-free expression. Nat Biotechnol 27: 747-752.
    • (2009) Nat Biotechnol , vol.27 , pp. 747-752
    • Mureev, S.1    Kovtun, O.2    Nguyen, U.T.3    Alexandrov, K.4
  • 43
    • 84862874922 scopus 로고    scopus 로고
    • Emerging technology of in situ cell free expression protein microarrays
    • Nand A, Gautam A, Pérez JB, Merino A, Zhu J (2012) Emerging technology of in situ cell free expression protein microarrays. Protein Cell 3: 84-88.
    • (2012) Protein Cell , vol.3 , pp. 84-88
    • Nand, A.1    Gautam, A.2    Pérez, J.B.3    Merino, A.4    Zhu, J.5
  • 45
    • 33751410139 scopus 로고    scopus 로고
    • Efficient protein selection based on ribosome display system with purified components
    • Ohashi H, Shimizu Y, Ying BW, Ueda T (2007) Efficient protein selection based on ribosome display system with purified components. Biochem Biophys Res Commun 352: 270-276.
    • (2007) Biochem Biophys Res Commun , vol.352 , pp. 270-276
    • Ohashi, H.1    Shimizu, Y.2    Ying, B.W.3    Ueda, T.4
  • 46
    • 84863705728 scopus 로고    scopus 로고
    • Cell-free protein synthesis from a single copy of DNA in a glass microchamber
    • Okano T, Matsuura T, Kazuta Y, Suzuki H, Yomo T (2012) Cell-free protein synthesis from a single copy of DNA in a glass microchamber. Lab Chip 12: 2704-2711.
    • (2012) Lab Chip , vol.12 , pp. 2704-2711
    • Okano, T.1    Matsuura, T.2    Kazuta, Y.3    Suzuki, H.4    Yomo, T.5
  • 48
    • 84857440637 scopus 로고    scopus 로고
    • High-yield cell-free protein synthesis for site-specific incorporation of unnatural amino acids at two sites
    • Ozawa K, Loscha KV, Kuppan KV, Loh CT, Dixon NE, Otting G (2012) High-yield cell-free protein synthesis for site-specific incorporation of unnatural amino acids at two sites. Biochem Biophys Res Commun 418: 652-656.
    • (2012) Biochem Biophys Res Commun , vol.418 , pp. 652-656
    • Ozawa, K.1    Loscha, K.V.2    Kuppan, K.V.3    Loh, C.T.4    Dixon, N.E.5    Otting, G.6
  • 49
    • 84555196342 scopus 로고    scopus 로고
    • Ribosome display: a perspective
    • Plückthun A (2012) Ribosome display: a perspective. Methods Mol Biol 805: 3-28.
    • (2012) Methods Mol Biol , vol.805 , pp. 3-28
    • Plückthun, A.1
  • 52
    • 0018350419 scopus 로고
    • Synthesis of high molecular weight thyroglobulin peptides by thyroid polysome in vitro
    • See YP, Burrow GN (1979) Synthesis of high molecular weight thyroglobulin peptides by thyroid polysome in vitro. Biochim Biophys Acta 561: 215-222.
    • (1979) Biochim Biophys Acta , vol.561 , pp. 215-222
    • See, Y.P.1    Burrow, G.N.2
  • 54
    • 23044504786 scopus 로고    scopus 로고
    • Protein synthesis by pure translation systems
    • Shimizu Y, Kanamori T, Ueda T (2005) Protein synthesis by pure translation systems. Methods 36: 299-304.
    • (2005) Methods , vol.36 , pp. 299-304
    • Shimizu, Y.1    Kanamori, T.2    Ueda, T.3
  • 56
    • 84855230373 scopus 로고    scopus 로고
    • Fermentative glycolysis with purified Escherichia coli enzymes for in vitro ATP production and evaluating an engineered enzyme
    • Stevenson BJ, Liu JW, Kuchel PW, Ollis DL (2012) Fermentative glycolysis with purified Escherichia coli enzymes for in vitro ATP production and evaluating an engineered enzyme. J Biotechnol 157: 113-123.
    • (2012) J Biotechnol , vol.157 , pp. 113-123
    • Stevenson, B.J.1    Liu, J.W.2    Kuchel, P.W.3    Ollis, D.L.4
  • 58
    • 18744389180 scopus 로고    scopus 로고
    • Photo-leucine and photo-methionine allow identification of protein-protein interactions
    • Suchanek M, Radzikowska A, Thiele C (2005) Photo-leucine and photo-methionine allow identification of protein-protein interactions. Nat Methods 2: 261-267.
    • (2005) Nat Methods , vol.2 , pp. 261-267
    • Suchanek, M.1    Radzikowska, A.2    Thiele, C.3
  • 59
    • 83255176666 scopus 로고    scopus 로고
    • Transforming biochemical engineering with cell-free biology
    • Swartz JR (2012) Transforming biochemical engineering with cell-free biology. AIChE J 58: 5-13.
    • (2012) AIChE J , vol.58 , pp. 5-13
    • Swartz, J.R.1
  • 60
    • 84872819599 scopus 로고    scopus 로고
    • Chapter 2, The constructive approach for cell-free translation
    • A. S. Spirin and J. R. Swartz (Eds.), Weinheim: Wiley-VCH Verlag GmbH & Co. KGaA
    • Ueda T (2008) Chapter 2, The constructive approach for cell-free translation. In: Spirin AS, Swartz JR (eds) Cell-free protein synthesis: methods and protocols. Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.
    • (2008) Cell-Free Protein Synthesis: Methods and Protocols
    • Ueda, T.1
  • 61
    • 77952751658 scopus 로고    scopus 로고
    • Ribosome display with the PURE technology
    • Ueda T, Kanamori T, Ohashi H (2010) Ribosome display with the PURE technology. Methods Mol Biol 607: 219-225.
    • (2010) Methods Mol Biol , vol.607 , pp. 219-225
    • Ueda, T.1    Kanamori, T.2    Ohashi, H.3
  • 62
    • 67849119369 scopus 로고    scopus 로고
    • Cell-free protein synthesis energized by slowly-metabolized maltodextrin
    • Wang Y, Zhang YH (2009) Cell-free protein synthesis energized by slowly-metabolized maltodextrin. BMC Biotechnol 9: 58.
    • (2009) BMC Biotechnol , vol.9 , pp. 58
    • Wang, Y.1    Zhang, Y.H.2
  • 64
    • 84555189972 scopus 로고    scopus 로고
    • Update on pure translation display with unnatural amino acid incorporation
    • Watts RE, Forster AC (2012) Update on pure translation display with unnatural amino acid incorporation. Methods Mol Biol 805: 349-365.
    • (2012) Methods Mol Biol , vol.805 , pp. 349-365
    • Watts, R.E.1    Forster, A.C.2
  • 65
    • 84855892823 scopus 로고    scopus 로고
    • Quality control in aminoacyl-tRNA synthesis its role in translational fidelity
    • Yadavalli SS, Ibba M (2012) Quality control in aminoacyl-tRNA synthesis its role in translational fidelity. Adv Protein Chem Struct Biol 86: 1-43.
    • (2012) Adv Protein Chem Struct Biol , vol.86 , pp. 1-43
    • Yadavalli, S.S.1    Ibba, M.2
  • 67
    • 79952452377 scopus 로고    scopus 로고
    • A practical method for cell-free protein synthesis to avoid stable isotope scrambling and dilution
    • Yokoyama J, Matsuda T, Koshiba S, Tochio N, Kigawa T (2011) A practical method for cell-free protein synthesis to avoid stable isotope scrambling and dilution. Anal Biochem 411: 223-229.
    • (2011) Anal Biochem , vol.411 , pp. 223-229
    • Yokoyama, J.1    Matsuda, T.2    Koshiba, S.3    Tochio, N.4    Kigawa, T.5
  • 68
    • 79956158054 scopus 로고    scopus 로고
    • Microscale to manufacturing scale-up of cell-free cytokine production-a new approach for shortening protein production development timelines
    • Zawada JF, Yin G, Steiner AR, Yang J, Naresh A, Roy SM, Gold DS, Heinsohn HG, Murray CJ (2011) Microscale to manufacturing scale-up of cell-free cytokine production-a new approach for shortening protein production development timelines. Biotechnol Bioeng 108: 1570-1578.
    • (2011) Biotechnol Bioeng , vol.108 , pp. 1570-1578
    • Zawada, J.F.1    Yin, G.2    Steiner, A.R.3    Yang, J.4    Naresh, A.5    Roy, S.M.6    Gold, D.S.7    Heinsohn, H.G.8    Murray, C.J.9
  • 69
    • 84870477768 scopus 로고    scopus 로고
    • Reconstitution of translation from Thermus thermophilus reveals a minimal set of components sufficient for protein synthesis at high temperatures and functional conservation of modern and ancient translation components
    • Zhou Y, Asahara H, Gaucher EA, Chong S (2012) Reconstitution of translation from Thermus thermophilus reveals a minimal set of components sufficient for protein synthesis at high temperatures and functional conservation of modern and ancient translation components. Nucleic Acids Res 40: 7932-7945.
    • (2012) Nucleic Acids Res , vol.40 , pp. 7932-7945
    • Zhou, Y.1    Asahara, H.2    Gaucher, E.A.3    Chong, S.4


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.