Oxalate improves protein synthesis by enhancing ATP supply in a cell-free system derived from Escherichia coli

Biotechnology Letters

Volumn 22, Issue 19, 2000, Pages 1537-1542

  Kim, Dong Myung ^a   Swartz, James R ^a  

^a Stanford University   (United States)

Author keywords

Acetyl phosphate; ATP regeneration; Cell free protein synthesis; Chloramphenicol acetyltransferase; Glucose 6 phosphate; Phosphoenolpyruvate; Pyruvate

Indexed keywords

ADENOSINE TRIPHOSPHATE; CHLORAMPHENICOL ACETYLTRANSFERASE; OXALIC ACID; PHOSPHOENOLPYRUVATE; SYNTHETASE;

ARTICLE; CELL FREE SYSTEM; ENERGY RESOURCE; ENZYME INHIBITION; ENZYME SYNTHESIS; ESCHERICHIA COLI; GENE DISRUPTION; NONHUMAN; PROTEIN SYNTHESIS;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 0033733369     PISSN: 01415492     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1005624811710     Document Type: Article

References (10)

1. Cloning and expression of the gene for bacteriophage T7 RNA polymerase
2. Recent advances in producing and selecting functional proteins by using cell-free translation
3. Cell-free synthesis and amino acid-selective stable isotope labeling of proteins for NMR analysis
4. Prolonging cell-free protein synthesis with a novel ATP regeneration system
5. Prolonging cell-free protein synthesis by selective reagent additions
6. Probes of the structure of phosphoenolpyruvate synthetase: Effects of a transition state analogue on enzyme conformation
7. In vitro method for the generation of protein libraries using PCR amplification of a single DNA molecule and coupled transcription/translation
8. Chloramphenicol acetyltransferase from chloramphenicol-resistant bacteria
9. The potentials of the in vitro biosynthesis system