Improving cell-free protein synthesis for stable-isotope labeling

Journal of Biomolecular NMR

Volumn 37, Issue 3, 2007, Pages 225-229

  Matsuda, Takayoshi ^a,b   Koshiba, Seizo ^a   Tochio, Naoya ^a   Seki, Eiko ^a,b   Iwasaki, Noriyuki ^a   Yabuki, Takashi ^a   Inoue, Makoto ^a   Yokoyama, Shigeyuki ^a,c   Kigawa, Takanori ^a,b  

^a RIKEN   (Japan)

^b TOKYO INSTITUTE OF TECHNOLOGY   (Japan)

^c UNIVERSITY OF TOKYO   (Japan)

Author keywords

Cell free protein synthesis; Invitro translation; Potassium D glutamate; Stable isotope labeling

Indexed keywords

ACETIC ACID; ASPARTIC ACID; GLUTAMIC ACID DERIVATIVE; GLUTAMINE; RAS PROTEIN;

ARTICLE; CELL FREE SYSTEM; CONCENTRATION RESPONSE; ESCHERICHIA COLI; ISOTOPE LABELING; NONHUMAN; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN SYNTHESIS; RNA TRANSLATION;

ASPARTIC ACID; CARBON ISOTOPES; CELL-FREE SYSTEM; ESCHERICHIA COLI; GLUTAMATES; ISOTOPE LABELING; ISOTOPES; MAGNETIC RESONANCE SPECTROSCOPY; NITROGEN ISOTOPES; PROLINE; PROTEIN BIOSYNTHESIS; PROTEINS; RAS PROTEINS;

ESCHERICHIA COLI;

EID: 33947572914     PISSN: 09252738     EISSN: 15735001     Source Type: Journal    
DOI: 10.1007/s10858-006-9127-5     Document Type: Article

References (18)

1. Bax A (1994) Multidimensional nuclear magnetic resonance methods for protein studies. Curr Opin Struct Biol 4:738-744
2. Delaglio F, Grzesiek S, Vuister GW, Zhu G, Pfeifer J, Bax A (1995) NMRPipe: A multidimensional spectral processing system based on UNIX pipes. J Biomol NMR 6:277-293
3. Jewett MC, Swartz JR (2004) Substrate replenishment extends protein synthesis with an in vitro translation system designed to mimic the cytoplasm. Biotechnol Bioeng 87:465-472
4. Johnson BA (2004) Using NMR View to visualize and analyze the NMR spectra of macromolecules. Methods Mol Biol 278:313-352
5. Kigawa T, Muto Y, Yokoyama S (1995) Cell-free synthesis and amino acid-selective stable isotope labeling of proteins for NMR analysis. J Biomol NMR 6:129-134
6. Kigawa T, Yabuki T, Yoshida Y, Tsutsui M, Ito Y, Shibata T, Yokoyama S (1999) Cell-free production and stable-isotope labeling of milligram quantities of proteins. FEBS Letters 442:15-19
7. Kigawa T, Yabuki T, Matsuda N, Matsuda T, Nakajima R, Tanaka A, Yokoyama S (2004) Preparation of Escherichia coli cell extract for highly productive cell-free protein expression. J Struct Funct Genomics 5:63-68
8. Kim DM, Kigawa T, Choi CY, Yokoyama S (1996) A highly efficient cell-free protein synthesis system from Escherichia coli. Eur J Biochem 239:881-886
9. Li H, Inoue M, Yabuki T, Aoki M, Seki E, Matsuda T, Nunokawa E, Motoda Y, Kobayashi A, Terada T, Shirouzu M, Koshiba S, Lin YJ, Guntert P, Suzuki H, Hayashizaki Y, Kigawa T, Yokoyama S (2005) Solution structure of the mouse enhancer of rudimentary protein reveals a novel fold. J Biomol NMR 32:329-334
10. Maeda T, Inoue M, Koshiba S, Yabuki T, Aoki M, Nunokawa E, Seki E, Matsuda T, Motoda Y, Kobayashi A, Hiroyasu F, Shirouzu M, Terada T, Hayami N, Ishizuka Y, Shinya N, Tatsuguchi A, Yoshida M, Hirota H, Matsuo Y, Tani K, Arakawa T, Carninci P, Kawai J, Hayashizaki Y, Kigawa T, Yokoyama S (2004) Solution structure of the SEA domain from the murine homologue of ovarian cancer antigen CA125 (MUC16). J Biol Chem 279:13174-13182
11. Matsuda T, Kigawa T, Koshiba S, Inoue M, Aoki M, Yamasaki K, Seki M, Shinozaki K, Yokoyama S (2006) Cell-free synthesis of zinc-binding proteins. J Struct Funct Genomics, (in press). DOI 10.1007/ s10969-006-9012-1
12. Ozawa K, Headlam MJ, Schaeffer PM, Henderson BR, Dixon NE, Otting G (2004) Optimization of an Escherichia coli system for cell-free synthesis of selectively N-labelled proteins for rapid analysis by NMR spectroscopy. Eur J Biochem 271:4084-4093
13. Spirin AS, Baranov VI, Ryabova LA, Ovodov SY, Alakhov YB (1988) A continuous cell-free translation system capable of producing polypeptides in high yield. Science 242:1162-1164
14. Vinarov DA, Lytle BL, Peterson FC, Tyler EM, Volkman BF, Markley JL (2004) Cell-free protein production and labeling protocol for NMR-based structural proteomics. Nature Methods 1:149-153
15. Wu PS, Ozawa K, Jergic S, Su XC, Dixon NE, Otting G (2006) Amino-acid type identification in 15 N-HSQC spectra by combinatorial selective 15 N-labelling. J. Biomol. NMR 34:13-21
16. Yamasaki K, Shirouzu M, Muto Y, Fujita-Yoshigaki J, Koide H, Ito Y, Kawai G, Hattori S, Yokoyama S, Nishimura S, Miyazawa T (1994) Site-directed mutagenesis, fluorescence, and two-dimensional NMR studies on microenvironments of effector region aromatic residues of human c-Ha-Ras protein. Biochemistry 33:65-73
17. Yamasaki K, Kigawa T, Inoue M, Tateno M, Yamasaki T, Yabuki T, Aoki M, Seki E, Matsuda T, Tomo Y, Hayami N, Terada T, Shirouzu M, Tanaka A, Seki M, Shinozaki K, Yokoyama S (2005) Solution structure of an Arabidopsis WRKY DNA binding domain. Plant Cell 17:944-956
18. Yokoyama S (2003) Protein expression systems for structural genomics and proteomics. Curr Opin Chem Biol 7:39-43