Alternative fermentation conditions for improved Escherichia coli-based cell-free protein synthesis for proteins requiring supplemental components for proper synthesis

Process Biochemistry

Volumn 49, Issue 2, 2014, Pages 217-222

  Smith, Mark Thomas ^a   Hawes, Anna Katz ^a   Shrestha, Prashanta ^a   Rainsdon, Jay Marvin ^a   Wu, Jeffrey C ^b   Bundy, Bradley Charles ^a  

^a Department of Electrical and Computer Engineering   (United States)

^b BRIGHAM YOUNG UNIVERSITY   (United States)

Author keywords

Candida antarctica lipase B; Cell free protein synthesis; Extract preparation; Heterologous expression; In vitro translation; Noncanonical amino acids

Indexed keywords

EID: 84895068367     PISSN: 13595113     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.procbio.2013.10.012     Document Type: Article

References (50)

1. E.D. Carlson, R. Gan, C.E. Hodgman, and M.C. Jewett Cell-free protein synthesis: applications come of age Biotechnol Adv 30 2012 1185 1194
2. T. Sawasaki, T. Ogasawara, R. Morishita, and Y. Endo A cell-free protein synthesis system for high-throughput proteomics Proc Natl Acad Sci USA 99 2002 14652 14657
3. D.C. Harris, and M.C. Jewett Cell-free biology: exploiting the interface between synthetic biology and synthetic chemistry Curr Opin Biotechnol 23 2012 672 678
4. C.E. Hodgman, and M.C. Jewett Cell-free synthetic biology: thinking outside the cell Metab Eng 14 2012 261 269
5. J.A. Rollin, T.K. Tam, and Y.H.P. Zhang New biotechnology paradigm: cell-free biosystems for biomanufacturing Green Chem 15 2013 1708 1719
6. J.F. Zawada, G. Yin, A.R. Steiner, J. Yang, A. Naresh, and S.M. Roy et al. Microscale to manufacturing scale-up of cell-free cytokine production - a new approach for shortening protein production development timelines Biotechnol Bioeng 108 2011 1570 1578
7. M.T. Smith, C.T. Varner, D.B. Bush, and B.C. Bundy The incorporation of the A2 protein to produce novel Qβ virus-like particles using cell-free protein synthesis Biotechnol Progr 28 2012 549 555
8. P. Avenaud, M. Castroviejo, S. Claret, J. Rosenbaum, F. Mégraud, and A. Ménard Expression and activity of the cytolethal distending toxin of Helicobacter hepaticus Biochem Biophys Res Commun 318 2004 739 745
9. J. Yang, G. Kanter, A. Voloshin, N. Michel-Reydellet, H. Velkeen, and R. Levy et al. Rapid expression of vaccine proteins for B-cell lymphoma in a cell-free system Biotechnol Bioeng 89 2005 503 511
10. K.G. Patel, P.P. Ng, S. Levy, R. Levy, and J.R. Swartz Escherichia coli-based production of a tumor idiotype antibody fragment - tetanus toxin fragment C fusion protein vaccine for B cell lymphoma Protein Exp Purif 75 2011 15 20
11. T. Matsuda, S. Furumoto, K. Higuchi, J. Yokoyama, M.-R. Zhang, and K. Yanai et al. Rapid biochemical synthesis of 11C-labeled single chain variable fragment antibody for immuno-PET by cell-free protein synthesis Bioorgan Med Chem 20 2012 6579 6582
12. H. Chandra, and S. Srivastava Cell-free synthesis-based protein microarrays and their applications Proteomics 10 2010 717 730
13. J.A. Stapleton, and J.R. Swartz A cell-free microtiter plate screen for improved [FeFe] hydrogenases PLoS ONE 5 2010 e10554
14. C.G. Park, M.A. Kwon, J.K. Song, and D.M. Kim Cell-free synthesis and multifold screening of Candida antarctica lipase B (CalB) variants after combinatorial mutagenesis of hot spots Biotechnol Progr 27 2011 47 53
15. K.R. Arnaz, J.C. Wu, B.C. Bundy, and M.C. Jewett Transforming synthetic biology with cell-free systems H. Zhao, Synthetic Biology: Tools and Applications 2013 Academic Press Waltham, MA 277 301
16. M.C. Jewett, K.A. Calhoun, A. Voloshin, J.J. Wuu, and J.R. Swartz An integrated cell-free metabolic platform for protein production and synthetic biology Mol Syst Biol 4 2008 220
17. Y.H.P. Zhang Production of biocommodities and bioelectricity by cell-free synthetic enzymatic pathway biotransformations: challenges and opportunities Biotechnol Bioeng 105 2010 663 677
18. S. Billerbeck, J. Härle, and S. Panke The good of two worlds: increasing complexity in cell-free systems Curr Opin Biotechnol 2013 10.1016/j.copbio.2013.03.007
19. A. Meyer, R. Pellaux, and S. Panke Bioengineering novel in vitro metabolic pathways using synthetic biology Curr Opin Microbiol 10 2007 246 253
20. G. Zubay In vitro synthesis of protein in microbial systems Annu Rev Genet 7 1973 267 287
21. K.A. Calhoun, and J.R. Swartz An economical method for cell-free protein synthesis using glucose and nucleoside monophosphates Biotechnol Progr 21 2005 1146 1153
22. D.V. Liu, J.F. Zawada, and J.R. Swartz Streamlining Escherichia coli S30 extract preparation for economical cell-free protein synthesis Biotechnol Progr 21 2005 460 465
23. T.W. Kim, J.W. Keum, I.S. Oh, C.Y. Choi, C.G. Park, and D.M. Kim Simple procedures for the construction of a robust and cost-effective cell-free protein synthesis system J Biotechnol 126 2006 554 561
24. W.C. Yang, K.G. Patel, H.E. Wong, and J.R. Swartz Simplifying and streamlining Escherichia coli-based cell-free protein synthesis Biotechnol Progr 28 2012 413 420
25. D. Schwarz, F. Junge, F. Durst, N. Frolich, B. Schneider, and S. Reckel et al. Preparative scale expression of membrane proteins in Escherichia coli-based continuous exchange cell-free systems Nat Protoc 2 2007 2945 2957
26. T.W. Kim, D.M. Kim, and C.Y. Choi Rapid production of milligram quantities of proteins in a batch cell-free protein synthesis system J Biotechnol 124 2006 373 380
27. B.C. Bundy, and J.R. Swartz Site-specific incorporation of p-propargyloxyphenylalanine in a cell-free environment for direct protein-protein click conjugation Bioconjug Chem 21 2010 255 263
28. C.-G. Park, T.-W. Kim, I.-S. Oh, J.K. Song, and D.-M. Kim Expression of functional Candida antarctica lipase B in a cell-free protein synthesis system derived from Escherichia coli Biotechnol Progr 25 2009 589 593
29. Q. Zheng, R. Shi, X. Zhu, L. Huang, J. Cai, and W. Han et al. Functional expression of Bacillus subtilis xylanase A in an Escherichia coli derived cell-free protein synthesis system and subsequent expression improvement via DNA gel technique Process Biochem 47 2012 1186 1191
30. Y. Shimizu, A. Inoue, Y. Tomari, T. Suzuki, T. Yokogawa, and K. Nishikawa et al. Cell-free translation reconstituted with purified components Nat Biotechnol 19 2001 751 755
31. P. Shrestha Streamlined extract preparation for E. coli-based cell-free protein synthesis and rapid site-specific incorporation of unnatural amino acids in proteins 2012 Master of Science; BrighamYoung University
32. D-E. Chang, D.J. Smalley, and T. Conway Gene expression profiling of Escherichia coli growth transitions: an expanded stringent response model Mol Microbiol 45 2002 289 306
33. J.-H. Seo, and J.E. Bailey Effects of recombinant plasmid content on growth properties and cloned gene product formation in Escherichia coli Biotechnol Bioeng 27 1985 1668 1674
34. C. Klammt, D. Schwarz, V. Dötsch, and F. Bernhard Cell-free production of integral membrane proteins on a preparative scale G. Grandi, In Vitro Transcription and Translation Protocols vol. 375 2007 Humana Press Totowa, New Jersey 57 78
35. C.L. Lee, D.S.W. Ow, and S.K.W. Oh Quantitative real-time polymerase chain reaction for determination of plasmid copy number in bacteria J Microbiol Methods 65 2006 258 267
36. R. Grabherr, E. Nilsson, G. Striedner, and K. Bayer Stabilizing plasmid copy number to improve recombinant protein production Biotechnol Bioeng 77 2002 142 147
37. J.R. Swartz Advances in Escherichia coli production of therapeutic proteins Curr Opin Biotechnol 12 2001 195 201
38. M. Yamagishi, H. Matsushima, A. Wada, M. Sakagmi, N. Fujita, and A. Ishihama Regulation of the Esherichia coli rmf gene encoding the ribosome modulation factor: growth phase- and growth rate-dependent control EMBO J 12 1993 625 630
39. T.S. Young, I. Ahmad, J.A. Yin, and P.G. Schultz An enhanced system for unnatural amino acid mutagenesis in E. coli J Mol Biol 395 2010 361 374
40. P. Shrestha, T.M. Holland, and B.C. Bundy Streamlined extract preparation for Escherichia coli-based cell-free protein synthesis by sonication or bead vortex mixing Biotechniques 53 2012 163 174
41. M.T. Smith, J.C. Wu, C.T. Varner, and B.C. Bundy Enhanced protein stability through minimally invasive, direct, covalent, and site-specific immobilization Biotechnol Progr 29 2013 247 254
42. B.C. Bundy, and J.R. Swartz Efficient disulfide bond formation in virus-like particles J Biotechnol 154 2011 230 239
43. M.C. Jewett, and J.R. Swartz Rapid expression and purification of 100 nmol quantities of active protein using cell-free protein synthesis Biotechnol Progr 20 2004 102 109
44. U.E. Cheah, W.A. Weigand, and B.C. Stark Effects of recombinant plasmid size on cellular processes in Escherichia coli Plasmid 18 1987 127 134
45. B.R. Glick Metabolic load and heterologous gene expression Biotechnol Adv 13 1995 247 261
46. R. Benveniste, and J. Davies Mechanisms of antibiotic resistance in bacteria Annu Rev Biochem 42 1973 471 506
47. C. Albayrak, and J.R. Swartz Using E. coli-based cell-free protein synthesis to evaluate the kinetic performance of an orthogonal tRNA and aminoacyl-tRNA synthetase pair Biochem Biophys Res Commun 431 2013 291 295
48. E.W. Hickey, and I.N. Hirshfield Low-pH-induced effects on patterns of protein synthesis and on internal pH in Escherichia coli and Salmonella typhimurium Appl Environ Microbiol 56 1990 1038 1045
49. P. Small, D. Balnkenhorn, D. Welty, E. Zinser, and J.L. Slonczewski Acid and base resistance in Escherichia coli and Shingella flexneri: role of rpoS and growth pH J Bacteriol 176 1994 1729 1737
50. A.R. Goerke, and J.R. Swartz High-level cell-free synthesis yields of proteins containing site-specific non-natural amino acids Biotechnol Bioeng 102 2009 400 416