A triple mutant in the Ω-loop of TEM-1 β-lactamase changes the substrate profile via a large conformational change and an altered general base for catalysis

Journal of Biological Chemistry

Volumn 290, Issue 16, 2015, Pages 10382-10394

  Stojanoski, Vlatko ^a   Chow, Dar Chone ^a   Hu, Liya ^a   Sankaran, Banumathi ^b   Gilbert, Hiram F ^a   Prasad, B V Venkataram ^a   Palzkill, Timothy ^a  

^a BAYLOR COLLEGE OF MEDICINE   (United States)

^b LAWRENCE BERKELEY NATIONAL LABORATORY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACIDS; ANTIBIOTICS; BACTERIA; CATALYSIS; CHAINS; DRUG PRODUCTS; ENZYMES; HYDROLYSIS; SEWAGE TREATMENT PLANTS;

BACTERIAL ENZYMES; CONFORMATIONAL CHANGE; ENZYME SPECIFICITY; GRAM-NEGATIVE BACTERIA; HYDROLYTIC MECHANISMS; RANDOM MUTAGENESIS; SUBSTRATE PROFILE; SUBSTRATE SPECIFICITY;

CRYSTAL STRUCTURE;

AMPICILLIN; BETA LACTAMASE TEM 1; CEFALOTIN; CEFTAZIDIME; CEPHALOSPORIN; HYDROXYL GROUP; NITROCEFIN; BETA LACTAMASE; BETA-LACTAMASE TEM-1; GLUTAMIC ACID; PENICILLIN DERIVATIVE; RECOMBINANT PROTEIN; TYROSINE;

AMINO ACID SUBSTITUTION; ARTICLE; BINDING SITE; CATALYSIS; CONFORMATIONAL TRANSITION; CONTROLLED STUDY; ENZYME ACTIVE SITE; ENZYME CONFORMATION; ENZYME SPECIFICITY; ENZYME STRUCTURE; ENZYME SUBSTRATE COMPLEX; HYDROLYSIS; MOLECULAR DOCKING; NONHUMAN; PH; PRIORITY JOURNAL; SITE DIRECTED MUTAGENESIS; STRUCTURE ANALYSIS; ANTIBIOTIC RESISTANCE; BIOCATALYSIS; CHEMICAL STRUCTURE; CHEMISTRY; ENZYMOLOGY; ESCHERICHIA COLI; GENE EXPRESSION; GENETICS; KINETICS; METABOLISM; MUTATION; PROTEIN SECONDARY STRUCTURE; PROTEIN TERTIARY STRUCTURE; STRUCTURE ACTIVITY RELATION; X RAY CRYSTALLOGRAPHY;

BACTERIA (MICROORGANISMS); NEGIBACTERIA;

BETA-LACTAM RESISTANCE; BETA-LACTAMASES; BIOCATALYSIS; CEFTAZIDIME; CRYSTALLOGRAPHY, X-RAY; ESCHERICHIA COLI; GENE EXPRESSION; GLUTAMIC ACID; HYDROGEN-ION CONCENTRATION; KINETICS; MODELS, MOLECULAR; MUTATION; PENICILLINS; PROTEIN STRUCTURE, SECONDARY; PROTEIN STRUCTURE, TERTIARY; RECOMBINANT PROTEINS; STRUCTURE-ACTIVITY RELATIONSHIP; SUBSTRATE SPECIFICITY; TYROSINE;

EID: 84927740260     PISSN: 00219258     EISSN: 1083351X     Source Type: Journal    
DOI: 10.1074/jbc.M114.633438     Document Type: Article

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