Characterization of free thiol variants of an IgG1 by reversed phase ultra high pressure liquid chromatography coupled with mass spectrometry

Journal of Pharmaceutical and Biomedical Analysis

Volumn 109, Issue , 2015, Pages 142-149

  Liu, Hongbin ^a   Jeong, Justin ^a   Kao, Yung Hsiang ^a   Zhang, Yonghua Taylor ^a  

^a GENENTECH INC   (United States)

Author keywords

Free thiol; LC MS; Recombinant monoclonal antibody; RP UHPLC

Indexed keywords

BIPHENYL; FORMIC ACID; IMMUNOGLOBULIN G1; MONOCLONAL ANTIBODY; RECOMBINANT ANTIBODY; RECOMBINANT MONOCLONAL ANTIBODY A; THIOL; UNCLASSIFIED DRUG; IMMUNOGLOBULIN G; RECOMBINANT PROTEIN; THIOL DERIVATIVE;

ARTICLE; DISULFIDE BOND; FLOW RATE; HYPERBARISM; LIQUID CHROMATOGRAPH; MOLECULAR WEIGHT; PARTICLE SIZE; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN CONFORMATION; REVERSED PHASE LIQUID CHROMATOGRAPHY; REVERSED PHASE ULTRA HIGH PRESSURE LIQUID CHROMATOGRAPHY; SIGNAL NOISE RATIO; TANDEM MASS SPECTROMETRY; ULTRA PERFORMANCE LIQUID CHROMATOGRAPHY; CHEMISTRY; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; POROSITY;

ANTIBODIES, MONOCLONAL; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; IMMUNOGLOBULIN G; MASS SPECTROMETRY; PARTICLE SIZE; POROSITY; RECOMBINANT PROTEINS; SULFHYDRYL COMPOUNDS;

EID: 84924412065     PISSN: 07317085     EISSN: 1873264X     Source Type: Journal    
DOI: 10.1016/j.jpba.2015.02.015     Document Type: Article

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