Identification and characterization of buried unpaired cysteines in a recombinant monoclonal igg1 antibody

Analytical Chemistry

Volumn 84, Issue 16, 2012, Pages 7112-7123

  Zhang, Taylor ^a   Zhang, Jennifer ^a   Hewitt, Daniel ^a   Tran, Ben ^a   Gao, Xiaoying ^b   Qiu, Zhihua Julia ^b   Tejada, Max ^c   Gazzano Santoro, Helene ^c   Kao, Yung Hsiang ^a  

^a Protein Analytical Chemistry   (United States)

^b BioAnalytical Sciences ^*  (United States)

^c GENENTECH INC   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BINDING ASSAYS; BINDING DATA; CELL-BASED; ENZYME LINKED IMMUNOSORBENT ASSAY; ION EXCHANGE CHROMATOGRAPHY; POTENCY ASSAY; REDOX STATUS; REVERSED-PHASE HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY; RP-HPLC; SIGNIFICANT IMPACTS; THERAPEUTIC ANTIBODIES;

CHROMATOGRAPHIC ANALYSIS; CHROMATOGRAPHY; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; MONOCLONAL ANTIBODIES;

AMINO ACIDS;

CD20 ANTIGEN; CYSTEINE; IMMUNOGLOBULIN G; MONOCLONAL ANTIBODY; RECOMBINANT PROTEIN; THIOL DERIVATIVE;

ANIMAL; ARTICLE; CHEMISTRY; CHO CELL; CRICETULUS; HAMSTER; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HUMAN; IMMUNOLOGY; ION EXCHANGE CHROMATOGRAPHY; MASS SPECTROMETRY; PROTEIN DENATURATION; REVERSED PHASE LIQUID CHROMATOGRAPHY;

ANIMALS; ANTIBODIES, MONOCLONAL; ANTIGENS, CD20; CHO CELLS; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; CHROMATOGRAPHY, ION EXCHANGE; CHROMATOGRAPHY, REVERSE-PHASE; CRICETINAE; CRICETULUS; CYSTEINE; HUMANS; IMMUNOGLOBULIN G; MASS SPECTROMETRY; PROTEIN DENATURATION; RECOMBINANT PROTEINS; SULFHYDRYL COMPOUNDS;

EID: 84865453635     PISSN: 00032700     EISSN: 15206882     Source Type: Journal    
DOI: 10.1021/ac301426h     Document Type: Article

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