Interchain disulfide bonding in human IgG2 antibodies probed by site-directed mutagenesis

Biochemistry

Volumn 48, Issue 17, 2009, Pages 3755-3766

  Allen, Martin J ^a,b   Guo, Amy ^b   Martinez, Theresa ^b   Han, Mei ^b   Flynn, Gregory C ^b   Wypych, Jette ^b   Liu, Yaoqing Diana ^b   Shen, Wenyan D ^b   Dillon, Thomas M ^b   Vezina, Christopher ^b   Balland, Alain ^b  

^a PFIZER INC   (United States)

^b AMGEN INC   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CATION EXCHANGE CHROMATOGRAPHY; CYSTEINE RESIDUES; DISULFIDE BONDING; DISULFIDE BONDS; HUMAN ANTIBODIES; IN-VITRO; ISOFORMS; RECOMBINANT MONOCLONAL ANTIBODY; REVERSED PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; SITE DIRECTED MUTAGENESIS; SODIUM DODECYL SULFATE-POLYACRYLAMIDE GEL ELECTROPHORESIS;

CHROMATOGRAPHY; ELECTROPHORESIS; GELATION; HIGH PRESSURE LIQUID CHROMATOGRAPHY; MONOCLONAL ANTIBODIES; MUTAGENESIS; SODIUM SULFATE;

CHROMATOGRAPHIC ANALYSIS;

CYSTEINE; IMMUNOGLOBULIN G ANTIBODY; IMMUNOGLOBULIN G2; IMMUNOGLOBULIN G2 ANTIBODY; MONOCLONAL ANTIBODY; MUTANT PROTEIN; RECOMBINANT ANTIBODY; UNCLASSIFIED DRUG;

ANTIBODY STRUCTURE; ARTICLE; CONTROLLED STUDY; DISULFIDE BOND; MOLECULAR PROBE; OXIDATION REDUCTION REACTION; PRIORITY JOURNAL; SITE DIRECTED MUTAGENESIS;

AMINO ACID SUBSTITUTION; ANTIBODIES, MONOCLONAL; CYSTEINE; DISULFIDES; ELECTROPHORESIS, POLYACRYLAMIDE GEL; HUMANS; IMMUNOGLOBULIN G; IMMUNOGLOBULIN KAPPA-CHAINS; MUTAGENESIS, SITE-DIRECTED; PEPTIDE FRAGMENTS; PEPTIDE MAPPING; RECOMBINANT PROTEINS; SERINE; SPECTROMETRY, MASS, ELECTROSPRAY IONIZATION;

EID: 66049135098     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi8022174     Document Type: Article

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