Impact of mobile phase temperature on recovery and stability of monoclonal antibodies using recent reversed-phase stationary phases

Journal of Separation Science

Volumn 35, Issue 22, 2012, Pages 3113-3123

  Fekete, Szabolcs ^a   Rudaz, Serge ^a   Veuthey, Jean Luc ^a   Guillarme, Davy ^a  

^a UNIVERSITY OF GENEVA   (Switzerland)

Author keywords

Adsorption; Core shell; Large biomolecules; Monoclonal antibodies; UHPLC

Indexed keywords

ACQUITY; ADSORPTION PHENOMENA; ANCILLARY SOLVENTS; BEVACIZUMAB; CORE-SHELL; CORE-SHELL PARTICLE; EQUIVALENT SIZE; HEAVY CHAIN; HYDROXYL GROUPS; LARGE BIOMOLECULES; MOBILE PHASE; N-BUTANOL; POROUS PARTICLE; RESIDENCE TIME; REVERSED PHASE; SECONDARY INTERACTIONS; SILANOL GROUPS; SILANOLS; STATIONARY PHASE; STATIONARY PHASIS; SYSTEMATIC INVESTIGATIONS; UHPLC;

ALCOHOLS; BIOMOLECULES; HYDROGEN BONDS; MONOCLONAL ANTIBODIES; POROUS MATERIALS; SURFACE PLASMON RESONANCE;

ADSORPTION;

BEVACIZUMAB; BUTANOL; IMMUNOGLOBULIN G1 ANTIBODY; IMMUNOGLOBULIN G2; MONOCLONAL ANTIBODY; PANITUMUMAB; RITUXIMAB;

ADSORPTION; ARTICLE; BIODEGRADATION; BIOLOGICAL MONITORING; CHEMICAL INTERACTION; FLOW RATE; FLUORESCENCE ANALYSIS; GEL PERMEATION CHROMATOGRAPHY; HEAVY CHAIN; HYDROGEN BOND; ION EXCHANGE; ION EXCHANGE CHROMATOGRAPHY; IONIC STRENGTH; LIGHT CHAIN; PARTICLE SIZE; PRIORITY JOURNAL; PROTEIN ANALYSIS; SEPARATION TECHNIQUE; TEMPERATURE;

ADSORPTION; ANTIBODIES, MONOCLONAL; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; CHROMATOGRAPHY, REVERSE-PHASE; IMMUNOGLOBULIN G; POROSITY; PROTEIN STABILITY; TEMPERATURE;

EID: 84866235919     PISSN: 16159306     EISSN: 16159314     Source Type: Journal    
DOI: 10.1002/jssc.201200297     Document Type: Article

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