An engineered autotransporter-based surface expression vector enables efficient display of Affibody molecules on OmpT-negative E. coli as well as protease-mediated secretion in OmpT-positive strains

Microbial Cell Factories

Volumn 13, Issue 1, 2014, Pages

  Fleetwood, Filippa ^a   Andersson, Ken G ^a   Ståhl, Stefan ^a   Löfblom, John ^a  

^a ROYAL INSTITUTE OF TECHNOLOGY   (Sweden)

Author keywords

Affibody molecule; AIDA I; Autotransporter; Bacterial display; Combinatorial protein engineering; Directed evolution; E. coli; FACS; Phage display; Secreted protein production

Indexed keywords

ALBUMIN; OUTER MEMBRANE PROTEASE T; PROTEINASE; UNCLASSIFIED DRUG; ADHESIN; AIDA-I PROTEIN, E COLI; BACTERIAL SECRETION SYSTEM; ESCHERICHIA COLI PROTEIN; HYBRID PROTEIN; OMPT PROTEIN, E COLI; OUTER MEMBRANE PROTEIN; PEPTIDE HYDROLASE;

ARTICLE; CONTROLLED STUDY; ENZYME RELEASE; ESCHERICHIA COLI; EXPRESSION VECTOR; FLUORESCENCE ACTIVATED CELL SORTING; GENE EXPRESSION SYSTEM; NONHUMAN; OUTER MEMBRANE; PROTEIN BINDING; PROTEIN PURIFICATION; PROTEIN SECRETION; BACTERIAL SECRETION SYSTEM; BIOSYNTHESIS; GENE EXPRESSION; GENE VECTOR; GENETICS; METABOLISM; PROTEIN TRANSPORT;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; NEGIBACTERIA;

ADHESINS, ESCHERICHIA COLI; BACTERIAL OUTER MEMBRANE PROTEINS; BACTERIAL SECRETION SYSTEMS; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; GENE EXPRESSION; GENETIC VECTORS; PEPTIDE HYDROLASES; PROTEIN TRANSPORT; RECOMBINANT FUSION PROTEINS;

EID: 84924087937     PISSN: None     EISSN: 14752859     Source Type: Journal    
DOI: 10.1186/s12934-014-0179-z     Document Type: Article

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