Modular organization of the AIDA autotransporter translocator: The N-terminal β1-domain is surface-exposed and stabilizes the transmembrane β2-domain

Antonie van Leeuwenhoek, International Journal of General and Molecular Microbiology

Volumn 80, Issue 1, 2001, Pages 19-34

  Konieczny, M P J ^a   Benz, I ^a   Hollinderbaumer B ^a   Beinke, C ^a   Niederweis, M ^a   Schmidt, M A ^a  

^a UNIVERSITY OF MÜNSTER   (Germany)

Author keywords

AIDA autotransporter; Circular dichroism; Epitope tagging; Heat modifiability; Membrane translocator; Renaturation; barrel

Indexed keywords

ADHESIN; CARRIER PROTEIN; CORE PROTEIN; DODECYL SULFATE SODIUM; EPITOPE; MEMBRANE PROTEIN; PEPTIDE;

BACTERIUM;

AMINO TERMINAL SEQUENCE; ARTICLE; BACTERIUM ADHERENCE; CARBOXY TERMINAL SEQUENCE; CONTROLLED STUDY; ESCHERICHIA COLI; ESCHERICHIA COLI 2787; FLUORESCENCE SPECTROSCOPY; GENE INSERTION; GRAM NEGATIVE BACTERIUM; IN VITRO STUDY; INCUBATION TIME; LIPID MEMBRANE; MEMBRANE STRUCTURE; MOLECULE; NONHUMAN; OUTER MEMBRANE; PRECURSOR; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN DENATURATION; PROTEIN DOMAIN; PROTEIN FOLDING; PROTEIN LOCALIZATION; PROTEIN PROCESSING; PROTEIN SECRETION; PROTEIN STRUCTURE; REPORTER GENE; TEMPERATURE;

ADHESINS, ESCHERICHIA COLI; BASE SEQUENCE; CELL MEMBRANE; CIRCULAR DICHROISM; ENDOPEPTIDASES; ESCHERICHIA COLI; HEAT; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; PROTEIN FOLDING; PROTEIN PRECURSORS; PROTEIN STRUCTURE, TERTIARY; RECOMBINANT FUSION PROTEINS; SEQUENCE DELETION; SPECTROMETRY, FLUORESCENCE;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; NEGIBACTERIA;

EID: 0035173780     PISSN: 00036072     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1012084325728     Document Type: Article

References (34)

1. Cloning and expression of an adhesin (AIDA-I) involved in diffuse adherence of enteropathogenic Escherichia coli
2. AIDA-I, the adhesin involved in diffuse adherence of the diarrhoeagenic Escherichia coli strain 2787 (O126:H27), is synthesized via a precursor molecule
3. Crystal structures explain functional properties of two E. coli porins
4. Solubilization of the cytoplasmic membrane of Escherichia coli by the ionic detergent sodium lauryl sarcosinate
5. Calculation and protein extinction coefficients from amino acid sequences
6. The great escape: Structure and function of the autotransporter proteins
7. Structure predictions of membrane proteins are not that bad
8. Structure and functional mechanism of porins
9. 1 protease-like outer membrane protein autotransporters
10. Selective extracellular release of cholera toxin B subunit by Escherichia coli: Dissection of Neisseria IgA β-mediated outer membrane transport
11. Characterization of the Neisseria Iga β-core
12. Membrane assembly of the outer membrane protein OmpA of Escherichia coli
13. Cell surface presentation of recombinant (poly-)peptides including functional T-cell epitopes by the AIDA autotransporter system
14. Autodisplay: Functional display of active β-lactamase on the surface of Escherichia coli by the AIDA-I autotransporter
15. A novel family of channel-forming, autotransporting, bacterial virulence factors
16. Effect of temperature and low pH on structure and stability of matrix porin in micellar detergent solutions
17. Autodisplay: One-component system for efficient surface display and release of soluble recombinant proteins from Escherichia coli
18. Characterization of the essential transport function of the AIDA-I autotransporter and evidence supporting structural predictions
19. Characterization of the precursor of Serratia marcescens serine protease and COOH-terminal processing of the precursor during its excretion through the outer membrane of Escherichia coli
20. Diarrheagenic Escherichia coli
21. Transport across the bacterial outer membrane
22. Structure of the outer membrane protein A transmembrane domain
23. Characterization of the major envelope protein of Escherichia coli
24. Expression of porin from Rhodopseudomonas blastica in Escherichia coli inclusion bodies and folding into exact native structure
25. The C-terminal domain of the Bordetella pertussis autotransporter BrkA forms a pore in lipid bilayer membranes
26. Characterization of secretory intermediates of Serratia marcescens serine protease produced during its extracellular secretion from Escherichia coli cells
27. Structure of staphylococcal α-hemolysin, a heptameric transmembrane pore
28. Crystal structures explain functional properties of two E. coli porins
29. C and evidence for the outer membrane anchoring as a β-barrel structure
30. Extracellular transport of VirG protein in Shigella
31. Probing secretion and translocation of a β-autotransporter using a reporter single-chain Fv as a cognate passenger domain
32. Models for the structure of outer-membrane proteins of Escherichia coli derived from raman spectroscopy and prediction methods
33. The structure of an antigenic determinant in a protein