Selection of Single Domain Antibodies from Immune Libraries Displayed on the Surface of E. coli Cells with Two β-Domains of Opposite Topologies

PLoS ONE

Volumn 8, Issue 9, 2013, Pages

  Salema, Valencio ^a   Marín, Elvira ^a   Martínez Arteaga, Rocio ^a   Ruano Gallego, David ^a   Fraile, Sofía ^a   Margolles, Yago ^a   Teira, Xema ^a   Gutierrez, Carlos ^b   Bodelón, Gustavo ^a   Fernández, Luis Ángel ^a  

^a CENTRO NACIONAL DE BIOTECNOLOGÍA   (Spain)

^b UNIVERSITY OF LAS PALMAS DE GRAN CANARIA   (Spain)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL PROTEIN; EHAA AUTOTRANSPORTER; INTIMIN; UNCLASSIFIED DRUG;

ANTIBODY AFFINITY; ANTIBODY LIBRARY; ANTIBODY STRUCTURE; ANTIGEN BINDING; ARTICLE; BACTERIAL CELL; CELL CLONE; CELL SURFACE DISPLAY; CONTROLLED STUDY; CYTOTOXICITY; ENTEROHEMORRHAGIC ESCHERICHIA COLI; ENZYME LINKED IMMUNOSORBENT ASSAY; ESCHERICHIA COLI K 12; ESCHERICHIA COLI O157; FLOW CYTOMETRY; IMMUNIZATION; MAGNETIC CELL SORTING; NONHUMAN; PROCEDURES CONCERNING CELLS; PROTEIN DEGRADATION; PROTEIN DOMAIN; PROTEIN PURIFICATION; SURFACE PLASMON RESONANCE;

ADHESINS, BACTERIAL; ADHESINS, ESCHERICHIA COLI; ANIMALS; ANTIBODY SPECIFICITY; BACTERIAL OUTER MEMBRANE PROTEINS; BLOTTING, WESTERN; CAMELS; ENZYME-LINKED IMMUNOSORBENT ASSAY; ESCHERICHIA COLI O157; ESCHERICHIA COLI PROTEINS; FLOW CYTOMETRY; OLIGONUCLEOTIDES; PEPTIDE LIBRARY; PLASMIDS; SINGLE-DOMAIN ANTIBODIES; SURFACE PLASMON RESONANCE;

EID: 84884523021     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0075126     Document Type: Article

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