Optimization of electroporation-mediated transformation: Staphylococcus carnosus as model organism

Journal of Applied Microbiology

Volumn 102, Issue 3, 2007, Pages 736-747

  Lofblom J ^a   Kronqvist, N ^a   Uhlen M ^a   Stahl S ^a   Wernerus H ^a  

^a ALBANOVA UNIVERSITY CENTER   (Sweden)

Author keywords

Affibody; Cell surface display; Electroporation; Gram positive; Staphylococcus carnosus; Transformation

Indexed keywords

BIOMOLECULES; CELL CULTURE; CELL MEMBRANES; DNA; ESCHERICHIA COLI; LIBRARIES; RECOMBINANT PROTEINS;

AFFIBODY; CELL SURFACE DISPLAYS; CELL SURFACES; ELECTROPORATION; GRAM POSITIVE; OPTIMISATIONS; PROTEIN LIBRARY; STAPHYLOCOCCUS CARNOSI; TRANSFORMATION; TRANSFORMATION FREQUENCY;

CELL PROLIFERATION;

PLASMID DNA; RECOMBINANT PROTEIN;

BACTERIUM; BIOTRANSFORMATION; DNA; GENE TRANSFER; OPTIMIZATION; PLASMID; PROTEOMICS;

ARTICLE; CELL POPULATION; CELL SURFACE; CONTROLLED STUDY; DNA TRANSFER; ELECTROPORATION; ESCHERICHIA COLI; FLOW CYTOMETRY; HOST CELL; NONHUMAN; PROTEIN EXPRESSION; STAPHYLOCOCCUS CARNOSUS;

ESCHERICHIA COLI; POSIBACTERIA; STAPHYLOCOCCUS; STAPHYLOCOCCUS CARNOSUS;

EID: 33847018452     PISSN: 13645072     EISSN: 13652672     Source Type: Journal    
DOI: 10.1111/j.1365-2672.2006.03127.x     Document Type: Article

References (37)

1. Augustin, J. and Götz, F. (1990) Transformation of Staphylococcus epidermidis and other staphylococcal species with plasmid DNA by electroporation. FEMS Microbiol Lett 54, 203-207.
2. Aukrust, T. and Blom, H. (1992) Transformation of Lactobacillus strains used in meat and vegetable fermentations. Food Res Int 25, 253-261.
3. Bessette, P.H., Rice, J.J. and Daugherty, P.S. (2004) Rapid isolation of high-affinity protein binding peptides using bacterial display. Protein Eng Des Sel 17, 731-739.
4. van den Beucken, T., Pieters, H., Steukers, M., van der Vaart, M., Ladner, R.C., Hoogenboom, H.R. and Hufton, S.E. (2003) Affinity maturation of Fab antibody fragments by fluorescent-activated cell sorting of yeast-displayed libraries. FEBS Lett 546, 288-294.
5. Buckley, N.D., Vadeboncoeur, C., LeBlanc, D.J., Lee, L.N. and Frenette, M. (1999) An effective strategy, applicable to Streptococcus salivarius and related bacteria, to enhance or confer electroporation competence. Appl Environ Microbiol 65, 3800-3804.
6. Cano, F., Plotnicky-Gilquin, H., Nguyen, T.N., Liljeqvist, S., Samuelson, P., Bonnefoy, J., Ståhl, S. and Robert, A. (2000) Partial protection to respiratory syncytial virus (RSV) elicited in mice by intranasal immunization using live staphylococci with surface- displayed RSV-peptides. Vaccine 18, 2743-2752.
7. Daugherty, P.S., Chen, G., Olsen, M.J., Iverson, B.L. and Georgiou, G. (1998) Antibody affinity maturation using bacterial surface display. Protein Eng 11, 825-832.
8. Dunny, G.M., Lee, L.N. and LeBlanc, D.J. (1991) Improved electroporation and cloning vector system for gram-positive bacteria. Appl Environ Microbiol 57, 1194-1201.
9. Edwards, R.A., Helm, R.A. and Maloy, S.R. (1999) Increasing DNA transfer efficiency by temporary inactivation of host restriction. Biotechniques 26, 892-898.
10. Feldhaus, M.J., Siegel, R.W., Opresko, L.K., Coleman, J.R., Feldhaus, J.M., Yeung, Y.A., Cochran, J.R., Heinzelman, P. et al. (2003) Flow-cytometric isolation of human antibodies from a nonimmune Saccharomyces cerevisiae surface display library. Nat Biotechnol 21, 163-170.
11. Georgiou, G., Stathopoulos, C., Daugherty, P.S., Nayak, A.R., Iverson, B.L. and Curtiss, R., 3rd (1997) Display of heterologous proteins on the surface of microorganisms: from the screening of combinatorial libraries to live recombinant vaccines. Nat Biotechnol 15, 29-34.
12. Harvey, B.R., Georgiou, G., Hayhurst, A., Jeong, K.J., Iverson, B.L. and Rogers, G.K. (2004) Anchored periplasmic expression, a versatile technology for the isolation of high-affinity antibodies from Escherichia coli-expressed libraries. Proc Natl Acad Sci USA 101, 9193-9198.
13. Holo, H. and Nes, I.F. (1989) High-frequency transformation, by electroporation, of Lactococcus lactis subsp. cremoris grown with glycine in osmotically stabilized media. Appl Environ Microbiol 55, 3119-3123.
14. Horinouchi, S. and Weisblum, B. (1982) Nucleotide sequence and functional map of pC194, a plasmid that specifies inducible chloramphenicol resistance. J Bacteriol 150, 815-825.
15. Kwak, J., Jiang, H. and Kendrick, K.E. (2002) Transformation using in vivo and in vitro methylation in Streptomyces griseus. FEMS Microbiol Lett 209, 243-248.
16. Lehtiö, J., Wernérus, H., Samuelson, P., Teeri, T.T. and Stå hl, S. (2001) Directed immobilization of recombinant staphylococci on cotton fibers by functional display of a fungal cellulose-binding domain. FEMS Microbiol Lett 195, 197-204.
17. Löfblom, J., Wernérus, H. and Stå hl, S. (2005) Fine affinity discrimination by normalized fluorescence activated cell sorting in staphylococcal surface display. FEMS Microbiol Lett 248, 189-198.
18. Nilsson, B., Moks, T., Jansson, B., Abrahmsén, L., Elmblad, A., Holmgren, E., Henrichson, C., Jones, T.A. et al. (1987) A synthetic IgG-binding domain based on staphylococcal protein A. Protein Eng 1, 107-113.
19. Nord, K., Gunneriusson, E., Ringdahl, J., Ståhl, S., Uhlén, M. and Nygren, P.Å. (1997) Binding proteins selected from combinatorial libraries of an alpha-helical bacterial receptor domain. Nat Biotechnol 15, 772-777.
20. Rüther, U. (1982) pUR 250 allows rapid chemical sequencing of both DNA strands of its inserts. Nucleic Acids Res 10, 5765-5772.
21. Schenk, S. and Laddaga, R.A. (1992) Improved method for electroporation of Staphylococcus aureus. FEMS Microbiol Lett 73, 133-138.
22. Scott, P.T. and Rood, J.I. (1989) Electroporation-mediated transformation of lysostaphin-treated Clostridium perfringens. Gene 82, 327-333.
23. Serror, P., Sasaki, T., Ehrlich, S.D. and Maguin, E. (2002) Electrotransformation of Lactobacillus delbrueckii subsp. bulgaricus and L. delbrueckii subsp. lactis with various plasmids. Appl Environ Microbiol 68, 46-52.
24. Shigekawa, K. and Dower, W.J. (1988) Electroporation of eukaryotes and prokaryotes: a general approach to the introduction of macromolecules into cells. Biotechniques 6, 742-751.
25. Smith, G.P. (1985) Filamentous fusion phage: novel expression vectors that display cloned antigens on the virion surface. Science 228, 1315-1317.
26. Smith, G.P. and Petrenko, V.A. (1997) Phage Display. Chem Rev 97, 391-410.
27. Suga, M. and Hatakeyama, T. (2003) High-efficiency electroporation by freezing intact yeast cells with addition of calcium. Curr Genet 43, 206-211.
28. Vaughan, T.J., Williams, A.J., Pritchard, K., Osbourn, J.K., Pope, A.R., Earnshaw, J.C., McCafferty, J., Hodits, R.A. et al. (1996) Human antibodies with sub-nanomolar affinities isolated from a large non-immunized phage display library. Nat Biotechnol 14, 309-314.
29. Vehmaanperä, J. (1989) Transformation of Bacillus amyloliquefaciens by electroporation. FEMS Microbiol Lett 52, 165-169.
30. Wernérus, H. and Stå hl, S. (2002) Vector engineering to improve a staphylococcal surface display system. FEMS Microbiol Lett 212, 47-54.
31. Wernérus, H. and Stå hl, S. (2004) Biotechnological applications for surface-engineered bacteria. Biotechnol Appl Biochem 40, 209-228.
32. Wernérus, H., Samuelson, P. and Stå hl, S. (2003) Fluorescence-activated cell sorting of specific affibody-displaying staphylococci. Appl Environ Microbiol 69, 5328-5335.
33. Wernérus, H., Lehtiö, J., Teeri, T., Nygren, P.Å. and Stå hl, S. (2001) Generation of metal-binding staphylococci through surface display of combinatorially engineered cellulosebinding domains. Appl Environ Microbiol 67, 4678-4684.
34. Wikman, M., Steffen, A.C., Gunneriusson, E., Tolmachev, V., Adams, G.P., Carlsson, J. and Stå hl, S. (2004) Selection and characterization of HER2/neu-binding affibody ligands. Protein Eng Des Sel 17, 455-462.
35. Wittrup, K.D. (2001) Protein engineering by cell-surface display. Curr Opin Biotechnol 12, 395-399.
36. Xue, G., Johnson, J.S. and Dalrymple, B.P. (1999) High osmolarity improves the electro-transformation efficiency of the gram-positive bacteria Bacillus subtilis and Bacillus licheniformis. J Microbiol Methods 34, 183-191.
37. Yoshimoto, H., Takahasi, Y., Kato, D. and Umemoto, T. (1997) Construction of a plasmid vector for transformation of Porphyromonas gingivalis. FEMS Microbiol Lett 152, 175-181.