Optimizing membrane protein overexpression in the Escherichia coli strain Lemo21(DE3)

Journal of Molecular Biology

Volumn 423, Issue 4, 2012, Pages 648-659

  Schlegel, Susan ^a   Löfblom, John ^b   Lee, Chiara ^c   Hjelm, Anna ^a   Klepsch, Mirjam ^a   Strous, Marc ^d,e   Drew, David ^c   Slotboom, Dirk Jan ^f   De Gier, Jan Willem ^a  

^a STOCKHOLM UNIVERSITY   (Sweden)

^b ALBANOVA UNIVERSITY CENTER   (Sweden)

^c IMPERIAL COLLEGE LONDON   (United Kingdom)

^d BIELEFELD UNIVERSITY   (Germany)

^e MAX PLANCK INSTITUTE FOR MARINE MICROBIOLOGY   (Germany)

^f UNIVERSITY OF GRONINGEN   (Netherlands)

Author keywords

membrane protein biogenesis; membrane protein functional structural studies; membrane protein production; optimization of protein expression; T7 RNA polymerase based overexpression

Indexed keywords

LYSOZYME; MEMBRANE PROTEIN; RNA POLYMERASE;

ARTICLE; BACTERIAL STRAIN; CHROMOSOME; ESCHERICHIA COLI; GENE EXPRESSION; NONHUMAN; PLASMID; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN EXPRESSION; PROTEIN FUNCTION;

ESCHERICHIA COLI;

EID: 84867268590     PISSN: 00222836     EISSN: 10898638     Source Type: Journal    
DOI: 10.1016/j.jmb.2012.07.019     Document Type: Article

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