Neutral sphingomyelinase-2 is a redox sensitive enzyme: Role of catalytic cysteine residues in regulation of enzymatic activity through changes in oligomeric state

Biochemical Journal

Volumn 465, Issue , 2015, Pages 371-382

  Dotson, P Patrick ^a   Karakashian, Alexander A ^a   Nikolova Karakashian, Mariana N ^a  

^a UNIVERSITY OF KENTUCKY   (United States)

Author keywords

Ceramide; Cysteine; Oligomerization; Oxidative stress; Sphingomyelinase; Thioredoxin

Indexed keywords

CYSTEINE; DITHIOTHREITOL; GLUTATHIONE; GLUTATHIONE DISULFIDE; HYDROGEN PEROXIDE; MONOMER; NEUTRAL SPHINGOMYELINASE 2; OLIGOMER; POLYMER; ROSETTA GAMI2; SPHINGOMYELIN PHOSPHODIESTERASE; THIOREDOXIN; THIOREDOXIN REDUCTASE; UNCLASSIFIED DRUG; SMPD3 PROTEIN, MOUSE;

ARTICLE; BACTERIAL STRAIN; CARBOXY TERMINAL SEQUENCE; CATALYSIS; CELL LYSATE; CELL MEMBRANE; DISULFIDE BOND; ELECTROPHORETIC MOBILITY; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ESCHERICHIA COLI; FLUORESCENCE RESONANCE ENERGY TRANSFER; GAIN OF FUNCTION MUTATION; GENE OVEREXPRESSION; HUMAN; HUMAN CELL; HYDROLYSIS; MUTATIONAL ANALYSIS; NONHUMAN; OLIGOMERIZATION; OXIDATION REDUCTION STATE; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN PROTEIN INTERACTION; SITE DIRECTED MUTAGENESIS; WESTERN BLOTTING; WILD TYPE; ANIMAL; CHEMISTRY; DRUG EFFECTS; ENZYME ACTIVATION; GENETICS; HEK293 CELL LINE; METABOLISM; MOUSE; OXIDATION REDUCTION REACTION; PHYSIOLOGY;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; MAMMALIA;

ANIMALS; CATALYSIS; CYSTEINE; ENZYME ACTIVATION; HEK293 CELLS; HUMANS; MICE; OXIDATION-REDUCTION; SPHINGOMYELIN PHOSPHODIESTERASE; THIOREDOXINS;

EID: 84921646331     PISSN: 02646021     EISSN: 14708728     Source Type: Journal    
DOI: 10.1042/BJ20140665     Document Type: Article

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