Purification and characterization of cloned alkaline protease gene of Geobacillus stearothermophilus

Journal of Basic Microbiology

Volumn 55, Issue 2, 2015, Pages 160-171

  Iqbal, Irfana ^a   Aftab, Muhammad Nauman ^a   Afzal, Mohammed ^b   Ur Rehman, Asad ^a   Aftab, Saima ^a   Zafar, Asma ^a   Ud Din, Zia ^a   Khuharo, Ateeque Rahman ^c   Iqbal, Jawad ^d   Ul Haq, Ikram ^a  

^a GC UNIVERSITY   (Pakistan)

^b KUWAIT UNIVERSITY   (Kuwait)

^c UNIVERSITY OF KARACHI   (Pakistan)

^d QUAID I AZAM UNIVERSITY   (Pakistan)

Author keywords

Alkaline serine protease; Characterization; Geobacillus stearothermophilus; Purification; Thermostable

Indexed keywords

ESCHERICHIA COLI; GEOBACILLUS STEAROTHERMOPHILUS;

ALKALINE PROTEINASE; BACTERIAL PROTEIN; BUTANOL; CASEIN; PROTEINASE; RECOMBINANT PROTEIN; SERINE PROTEINASE; SURFACTANT;

CHEMISTRY; ENZYME ACTIVE SITE; ENZYME SPECIFICITY; ENZYME STABILITY; ENZYMOLOGY; ESCHERICHIA COLI; FERMENTATION; GENETICS; GEOBACILLUS STEAROTHERMOPHILUS; GROWTH, DEVELOPMENT AND AGING; ION EXCHANGE CHROMATOGRAPHY; ISOLATION AND PURIFICATION; METABOLISM; MOLECULAR CLONING; MOLECULAR WEIGHT; PH; POLYACRYLAMIDE GEL ELECTROPHORESIS; TEMPERATURE;

1-BUTANOL; BACTERIAL PROTEINS; CASEINS; CATALYTIC DOMAIN; CHROMATOGRAPHY, ION EXCHANGE; CLONING, MOLECULAR; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ENDOPEPTIDASES; ENZYME STABILITY; ESCHERICHIA COLI; FERMENTATION; GEOBACILLUS STEAROTHERMOPHILUS; HYDROGEN-ION CONCENTRATION; MOLECULAR WEIGHT; RECOMBINANT PROTEINS; SERINE PROTEASES; SUBSTRATE SPECIFICITY; SURFACE-ACTIVE AGENTS; TEMPERATURE;

EID: 84921470732     PISSN: 0233111X     EISSN: 15214028     Source Type: Journal    
DOI: 10.1002/jobm.201400190     Document Type: Article

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