Characterization of a recombinant thermostable xylanase from deep-sea thermophilic Geobacillus sp. MT-1 in East Pacific

Applied Microbiology and Biotechnology

Volumn 72, Issue 6, 2006, Pages 1210-1216

  Wu, Suijie ^a,b   Liu, Bin ^b   Zhang, Xiaobo ^b  

^a XIAMEN UNIVERSITY   (China)

^b Key Laboratory of Marine Genetic Resources of Fujian Province   (China)

Author keywords

[No Author keywords available]

Indexed keywords

HYDROTHERMAL FIELDS; RECOMBINANT XYLANASE; THERMOPHILIC GEOBACILLUS; THERMOPHILIC STRAIN;

BACTERIA; GENES; GENETIC ENGINEERING; HYDROTHERMAL SYNTHESIS; PH EFFECTS; PURIFICATION; WATER BACTERIOLOGY;

ENZYMES;

ALUMINUM; BENZYLSULFONYL FLUORIDE; CADMIUM; COBALT; COPPER ION; DITHIOTHREITOL; EDETIC ACID; ENZYME INHIBITOR; INDUSTRIAL ENZYME; LITHIUM ION; MANGANESE; MERCAPTOETHANOL; MERCURY; NICKEL; POLYSORBATE 20; POTASSIUM ION; RECOMBINANT ENZYME; SODIUM ION; TRITON X 100; XYLAN; XYLAN ENDO 1,3 BETA XYLOSIDASE; ZINC ION;

AMINO ACID; DEEP SEA; ENZYME; THERMOPHILIC BACTERIUM;

AMINO ACID SEQUENCE; APPLIED RESEARCH; ARTICLE; BACTERIAL STRAIN; BACTERIUM ISOLATION; CONTROLLED STUDY; DEEP SEA; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME INHIBITION; ENZYME KINETICS; ENZYME MECHANISM; ENZYME PURIFICATION; ENZYME STABILITY; ENZYME SYNTHESIS; ESCHERICHIA COLI; GENE; GENE EXPRESSION; GENE OVEREXPRESSION; GEOBACILLUS; INDUSTRY; MICHAELIS CONSTANT; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; OAT; PH; PROTEIN EXPRESSION; TEMPERATURE; THERMOPHILIC BACTERIUM;

BACILLACEAE; BACTERIAL PROTEINS; CLONING, MOLECULAR; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ENDO-1,4-BETA XYLANASES; ENZYME INHIBITORS; ENZYME STABILITY; ESCHERICHIA COLI; HYDROGEN-ION CONCENTRATION; KINETICS; METALS; PACIFIC OCEAN; RECOMBINANT PROTEINS; SEAWATER; SODIUM DODECYL SULFATE; TEMPERATURE; XYLANS;

PACIFIC OCEAN; PACIFIC OCEAN (EAST);

ESCHERICHIA COLI; GEOBACILLUS SP. MT-1; TRITICUM AESTIVUM SUBSP. SPELTA;

EID: 33749835413     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00253-006-0416-4     Document Type: Article

References (27)

1. Bailey MJ, Biely P, Poutanen K (1992) Interlaboratory testing of methods for assay of xylanase activity. J Biotechnol 23:257-270
2. Beg QK, Kapoor M, Mahajan L, Hoondal GS (2001) Microbial xylanases and their industrial applications. Appl Microbiol Biotechnol 56:326-338
3. Bergquist PL, Gibbs MD, Morris DD, TeÓ VSJ, Saul DJ, Morgan HW (1999) Molecular diversity of thermophilic cellulolytic and hemicellulolytic bacteria. FEMS Microbiol Ecol 28:99-110
4. Biely P (1985) Microbial xylanolytic systems. Trends Biotechnol 3:286-290
5. Blanco A, Díaz P, Zueco J, Parascandola P, Pastor FIJ (1999) A multidomain xylanase from a Bacillus sp. with a region homologous to thermostabilizing domains of thermophilic enzymes. Microbiology 145:2163-2170
6. Bradford MM (1976) A rapid sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248-254
7. Christakopoulos P, Katapodis P, Kalogeris E, Kekos D, Macris BJ, Stamatis H, Skaltsa H (2003) Antimicrobial activity of acidic xylooligosaccharides produced by family 10 and 11 endoxylanases. Int J Biol Macromol 31:171-175
8. Clarke JH, Davidson K, Gilbert HJ, Fontes C, Hazlewood GP (1996) A modular xylanase from mesophilic Cellulomonas fimi contains the same cellulose-binding and thermostabilizing domains as xylanases from thermophilic bacteria. FEMS Microbiol Lett 139:27-35
9. Connerton I, Cumming N, Harris GW, Debeire P, Breton C (1999) A single domain thermophilic xylanase can bind insoluble xylan: evidence for surface aromatic clusters. Biochim Biophys Acta 1433:110-121
10. Fontes CM, Hazlewood GP, Morag E, Hall J, Hirst BH, Gilbert HJ (1995). Evidence for a general role for non-catalytic thermostabilizing domains in xylanases from thermophilic bacteria. Biochem J 307:151-158
11. Gibbs MD, Reeves RA, Bergquist PL (1995) Cloning sequencing and expression of a xylanase Gene from the extreme thermophile Dictyoglomus thermophilum Rt46B.1 and activity of the enzyme on fiber-bound substrate. Appl Environ Microbiol 61:4403-4408
12. Gilkes NR, Henrissat B, Kilburn DG, Miller RC, Warren RAJ (1991) Domains in microbial β-1,4-glycanases; sequence conservation, function, and enzyme families. Microbiol Rev 55:303-315
13. Khanna S, Gauri P (1993) Regulation, purification and properties of xylanase from Cellulomonas fimi. Enzyme Microb Technol 15:990-995
14. Kulkarni N, Shendye A, Rao M (1999) Molecular and biotechnological aspects of xylanases. FEMS Microbiol Rev 23:411-456
15. Lineweaver H, Burk D (1934) The determination of enzyme dissociation constants. J Am Chem Soc 57:685
16. Martínez MA, Delgado OD, Baigorí MD, Siòeriz F (2005) Sequence analysis, cloning and over-expression of an endoxylanase from the alkaliphilic Bacillus halodurans. Biotechnol Lett 27:545-550
17. Miller GL (1959) Use of dinitrosalicylic reagent for determination of reducing sugars. Anal Chem 31:426-428
18. Oppenheimer CH, ZoBell CE (1952) The growth and viability of sixty-three species of marine bacteria as influenced by hydrostatic pressure. J Mar Res 11:10-18
19. Shah AR, Madamwar D (2005) Xylanase production by a newly isolated Aspergillus foetidus strain and its characterization. Process Biochem 40:1763-1771
20. Shibuya H, Kaneko S, Hayashi K (2000) Enhancement of the thermostability and hydrolytic activity of xylanase by random gene shuffling. Biochem J 349:651-656
21. Subramaniyan S, Prema P (2002) Biotechnology of microbial xylanases: enzymology, molecular biology, and application. Crit Rev Biotechnol 22:33-64
22. Sunna A, Antranikian G (1997) Xylanolytic enzymes from fungi and bacteria. Crit Rev Biotechnol 17:39-67
23. Sunna A, Gibbs MD, Bergquist PL (2000) A novel thermostable multidomain 1,4-β-xylanase from 'Caldibacillus cellulovorans' and effect of its xylan-binding domain on enzyme activity. Microbiology 146:2947-2955
24. Viikari L, Kantelineo A, Bundquist J, Linko M (1994) Xylanase in bleaching: from an idea to the industry. FEMS Microbiol Rev 13:335-350
25. Viikari L, Sundquist J, Kettunen J (1991) Xylanase enzymes promote pulp bleaching. Pap Puu (Pap Tim) 73:384-389
26. Wong KY, Tan LUL, Saddler JN (1988) Multiplicity of β-1,4-xylanase in microorganisms: functions and applications. Microbiol Rev 52:305-317
27. Xiaoyan W, Jianyi S (2005) Construction, expression and characterization of a thermostable xylanase. Curr Microbiol 51:188-192