Cloning and expression of islandisin, a new thermostable subtilisin from Fervidobacterium islandicum, in Escherichia coli

Applied and Environmental Microbiology

Volumn 71, Issue 7, 2005, Pages 3951-3958

  Gödde, Carolin ^a   Sahm, Kerstin ^a   Brouns, Stan J J ^b   Kluskens, Leon D ^b   Van Der Oost, John ^b   De Vos, Willem M ^b   Antranikian, Garabed ^a  

^a HAMBURG UNIVERSITY OF TECHNOLOGY   (Germany)

^b WAGENINGEN UNIVERSITY   (Netherlands)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACIDS; BACTERIA; CHROMATOGRAPHY; CLONING; DETERGENTS; ENZYMES; GENES; HYDROXYAPATITE; PH EFFECTS; SOLVENTS;

CATALYTIC RESIDUES; FERVIDOBACTERIUM ISLANDICUM (DSMZ 5733); GENE ENCODING; OPEN READING FRAME;

ESCHERICHIA COLI;

ASPARAGINE; HISTIDINE; ISLANDISIN; SERINE PROTEINASE; SIGNAL PEPTIDE; SUBTILISIN; UNCLASSIFIED DRUG;

BIOTECHNOLOGY; GENE EXPRESSION;

AMINO ACID SEQUENCE; ARTICLE; CHROMATOGRAPHY; CONTROLLED STUDY; DENATURATION; ENZYME ASSAY; ENZYME DEGRADATION; ENZYME PURIFICATION; ESCHERICHIA COLI; FERVIDOBACTERIUM ISLANDICUM; GENE AMPLIFICATION; GENE EXPRESSION; GENETIC CODE; HALF LIFE TIME; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; OPEN READING FRAME; PH; PHYLOGENY; POLYMERASE CHAIN REACTION; SEQUENCE ANALYSIS; SEQUENCE HOMOLOGY; TEMPERATURE DEPENDENCE; THERMOPHILIC BACTERIUM; THERMOSTABILITY;

AMINO ACID SEQUENCE; BACTERIA; BASE SEQUENCE; CLONING, MOLECULAR; ENZYME STABILITY; ESCHERICHIA COLI; HEAT; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; PHYLOGENY; SEQUENCE ANALYSIS, DNA; SUBTILISIN;

ESCHERICHIA COLI; FERVIDOBACTERIUM ISLANDICUM;

EID: 22144455035     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/AEM.71.7.3951-3958.2005     Document Type: Article

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