A novel surfactant-stable alkaline serine-protease from a newly isolated Bacillus mojavensis A21. Purification and characterization

Process Biochemistry

Volumn 44, Issue 1, 2009, Pages 29-35

  Haddar, Anissa ^a   Bougatef, Ali ^a   Agrebi, Rym ^a   Sellami Kamoun, Alya ^a   Nasri, Moncef ^a  

^a UNIVERSITY OF SFAX   (Tunisia)

Author keywords

Alkaline protease; Bacillus mojavensis; Purification; Surfactant stable

Indexed keywords

ACETONE; AMINATION; AMINES; AMINO ACIDS; ANIONIC SURFACTANTS; BACTERIOLOGY; CALCIUM CHLORIDE; CHROMATOGRAPHIC ANALYSIS; CLEANING; COLLOIDS; DETERGENTS; DIFFUSERS (OPTICAL); ENZYMES; FARM BUILDINGS; GEL PERMEATION CHROMATOGRAPHY; GELATION; GELS; ION CHROMATOGRAPHY; ION EXCHANGE; IONIZATION OF LIQUIDS; ORGANIC ACIDS; PH EFFECTS; POROUS MATERIALS; PURIFICATION; SOAPS (DETERGENTS); SURFACE ACTIVE AGENTS;

16S RRNA GENE SEQUENCING; ALKALINE PROTEASE; AMINO ACID SEQUENCES; BACILLUS MOJAVENSIS; BIOCHEMICAL PROPERTIES; CULTURE SUPERNATANT; EXTRACELLULAR; GEL FILTRATIONS; HIGH STABILITIES; INITIAL ACTIVITIES; ION EXCHANGE CHROMATOGRAPHIES; LAUNDRY DETERGENTS; LIQUID DETERGENTS; MARINE WATERS; MOJAVENSIS; OXIDIZING AGENTS; PH RANGES; POTENTIAL APPLICATIONS; PURIFIED ENZYMES; RELATIVE ACTIVITIES; SEPHADEX; SEPHAROSE; SERINE PROTEASES; SODIUM PERBORATES; SPECIFIC ACTIVITIES; SURFACTANT-STABLE; TRITON X-100;

ENZYME ACTIVITY;

BACILLUS (BACTERIUM); BACILLUS MOJAVENSIS; BACTERIA (MICROORGANISMS);

EID: 58049217428     PISSN: 13595113     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.procbio.2008.09.003     Document Type: Article

References (37)

1. Godfrey T., and West S. Industrial enzymology. 2nd ed. (1996), McMillan Publishers Inc., New York 3 pp
2. Kumar C.G., and Takagi H. Microbial alkaline proteases from a bioindustrial viewpoint. Biotechnol Adv 17 (1999) 561-594
3. Gupta R., Beg Q., and Lorenz P. Bacterial alkaline proteases: molecular approaches and industrial applications. Appl Microbiol Biotechnol 59 (2002) 15-32
4. Gupta R., Gupta K., Saxena R.K., and Khan S. Bleach-stable, alkaline protease from Bacillus sp. Biotechnol Lett 21 (1999) 135-138
5. Kalisz H.M. Microbial proteinases. Adv Biochem Eng Biotechnol 36 (1988) 1-65
6. Maurer K.H. Detergent proteases. Curr Opin Biotechnol 15 (2004) 330-334
7. Bhaskar N., Sudeepa E.S., Rashmi H.N., and Tamil Selvi A. Partial purification and characterization of protease of Bacillus proteolyticus CFR3001 isolated from fish processing waste and its antibacterial activities. Bioresource Technol 98 (2007) 2758-2764
8. Jacobs M., Elkiasson M., Uhlen M., and Flock J.I. Cloning, sequencing and expression of Subtilisin Carlsberg from Bacillus licheniformis. Nucleic Acids Res 13 (1985) 8913-8927
9. Wells J.A., Ferrari E., Henner D.J., Estell D.A., and Chen E.Y. Cloning sequencing and secretion of Bacillus amyloliquefaciens subtilisin in Bacillus subtilis. Nucleic Acids Res 11 (1983) 7911-7929
10. Horikoshi K. Enzymes of alkalophiles. In: St Louis M.O. (Ed). Microbial enzymes and biotechnology. 2nd ed. (1990), Elsevier Applied Science, Amsterdam 275-294
11. Hajji M., Kanoun S., Nasri M., and Gharsallah N. Purification and characterization of an alkaline serine-protease produced by a new isolated Aspergillus clavatus ES1. Process Biochem 72 (2007) 791-797
12. Beg Q.K., and Gupta R. Purification and characterization of an oxidation-stable, thiol-dependent serine alkaline protease from Bacillus mojavensis. Enzyme Microb Technol 32 (2003) 294-304
13. Sanger F., Niklen S., and Coulson A.R. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci USA 74 (1977) 5463-5467
14. Altschul S.F., Madden T.L., Schäffer A.A., Zhang J., Zhang Z., Miller W., et al. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res 25 (1997) 3389-3402
15. Miller J.H. Experiments in moleculer genetics (1972), Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY pp. 431-435
16. Kembhavi A.A., Kulkarni A., and Pant A. Salt-tolerant and thermostable alkaline protease from Bacillus subtilis NCIM No. 64. Appl Biochem Biotechnol 38 (1993) 83-92
17. Laemmli U.K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227 (1970) 680-685
18. Garcia-Carreno F.L., Dimes L.F., and Haard N.F. Substrate-gel electrophoresis for composition and molecular weight of protease and proteinaceous. Anal Biochem 214 (1993) 65-69
19. Bradford M.M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72 (1976) 248-254
20. Adinarayana K., Ellaiah P., and Prasad D.S. Purification and partial characterization of thermostable serine alkaline protease from a newly isolated Bacillus subtilis PE-11. AAPS Pharm Sci Technol 4 (2003) 1-9
21. Steele D.B., Fiske M.J., Steele B.P., and Kelley V.C. Production of a low-molecular-weight, alkaline-active, thermostable protease by a novel spiral-shaped bacterium Kurthia spiroforme sp. Nov. Enzyme Microb Technol 14 (1992) 358-360
22. Winters P., Caldwell R., Enfield L., and Ferrari E. The ampS-nprE (124 degrees-127 degrees) region of the Bacillus subtilis 168 chromosome: sequencing of a 27 kb segment and identification of several genes in the area. Microbiology 142 (1996) 3033-3037
23. Chen X.H., Koumoutsi A., Scholz R., Eisenreich A., Schneider K., Heinemeyer I., et al. Comparative analysis of the complete genome sequence of the plant growth-promoting bacterium Bacillus amyloliquefaciens FZB42. Nat Biotechnol 25 (2007) 1007-1014
24. Hu X., Fan W., Han B., Liu H., Zheng D., Li Q., et al. Complete genome sequence of the mosquitocidal bacterium Bacillus sphaericus C3-41 and comparison with those of closely related Bacillus species. J Bacteriol 190 (2008) 2892-2902
25. Rey M.W., Ramaiya P., Nelson B.A., Brody-Karpin S.D., Zaretsky E.J., Tang M., et al. Complete genome sequence of the industrial bacterium Bacillus licheniformis and comparisons with closely related Bacillus species. Genome Biol 5 (2004) R77
26. Venugopal M., and Saramma A.V. Characterization of alkaline protease from Vibrio fluvialis strain VM10 isolated from a mangrove sediment sample and its application as a laundry detergent additive. Process Biochem 41 (2006) 1239-1243
27. Amoozegar M.A., Fatemi A.Z., Karbalaei-Heidari H.R., and Razavi M.R. Production of an extracellular alkaline metalloprotease from a newly isolated, moderately halophile, Salinivibrio sp. strain AF-2004. Microbiol Res 162 (2007) 369-377
28. Sana B., Ghosh D., Saha M., and Mukherjee J. Purification and characterization of a salt, solvent, detergent and bleach tolerant protease from a new gamma-Proteobacterium isolated from the marine environment of the Sundarbans. Process Biochem 41 (2006) 208-215
29. Kumar C.G. Purification and characterization of a thermostable alkaline protease from alkalophilic Bacillus pumilus. Lett Appl Microbiol 34 (2002) 13-17
30. Ghorbel-Frikha B., Sellami-Kamoun A., Fakhfakh N., Haddar A., Manni L., and Nasri M. Production and purification of a calcium-dependent protease from Bacillus cereus BG1. J Ind Microbiol Biotechnol 32 (2005) 186-194
31. 2+ binding. Biophys J 81 (2001) 2972-2978
32. Vieille C., and Zeikus J.G. Hyperthermophilic enzymes: sources, uses, and molecular mechanisms for thermostability. Microbiol Mol Biol Rev 65 (2001) 1-43
33. Deane S.M., Robb F.T., and Woods D.R. Production and activation of an SDS-resistant alkaline serine exoprotease of Vibrio alginolyticus. J Gen Microbiol 133 (1987) 391-398
34. Oberoi R., Beg Q.K., Puri S., Saxena R.K., and Gupta R. Characterization and wash performance analysis of an SDS-stable alkaline protease from a Bacillus sp. World J Microbiol Biotechnol 17 (2001) 493-497
35. Sellami-Kamoun A., Haddar A., El-Hadj Ali N., Ghorbel-Frikha B., Kanoun S., and Nasri M. Stability of thermostable alkaline protease from Bacillus licheniformis RP1 in commercial solid laundry detergent formulations. Microbiol Res 163 (2008) 299-306
36. Singh J., Batra N., and Sobti R.C. Serine alkaline protease from a newly isolated Bacillus sp. SSR1. Process Biochem 36 (2001) 781-785
37. Bhosale S.H., Rao M.B., Deshpande V.V., and Srinivasan M.C. Thermostability of high-activity alkaline protease from Conidiobolus coronatus (NCL 86.8.20). Enzyme Microb Technol 17 (1995) 136-139