Partial purification and characterization of protease of Bacillus proteolyticus CFR3001 isolated from fish processing waste and its antibacterial activities

Bioresource Technology

Volumn 98, Issue 14, 2007, Pages 2758-2764

  Bhaskar, N ^a   Sudeepa, E S ^a   Rashmi, H N ^a   Tamil Selvi, A ^a  

^a CENTRAL FOOD TECHNOLOGICAL RESEARCH INSTITUTE   (India)

Author keywords

Alkaline protease; Antibacterial activity; Bacillus proteolyticus; Partial purification

Indexed keywords

ENZYMES; ESCHERICHIA COLI; FISH; MOLECULAR WEIGHT; PH EFFECTS; PURIFICATION;

ALKALINE PROTEASE; ANTIBACTERIAL ACTIVITY; BACILLUS PROTEOLYTICUS; PARTIAL PURIFICATION;

LIVING SYSTEMS STUDIES;

ALKALINE PROTEINASE; FRESH WATER; PROTEINASE; SEA WATER;

ENZYMES; ESCHERICHIA COLI; FISH; LIVING SYSTEMS STUDIES; MOLECULAR WEIGHT; PH EFFECTS; PURIFICATION;

ALKALINITY; ANTIMICROBIAL ACTIVITY; BACTERIUM; ENZYME; FISH; INDUSTRIAL WASTE; INHIBITION; PATHOGENICITY; SCANNING ELECTRON MICROSCOPY;

ANTIBACTERIAL ACTIVITY; ARTICLE; BACILLUS; BACILLUS CEREUS; BACILLUS PROTEOLYTICUS; BACTERIAL CELL; BACTERIOLYSIS; CELL GROWTH; ENZYME ANALYSIS; ENZYME ISOLATION; ENZYME PURIFICATION; ESCHERICHIA COLI; FISH; LISTERIA MONOCYTOGENES; MOLECULAR WEIGHT; NONHUMAN; PH; PRIORITY JOURNAL; SCANNING ELECTRON MICROSCOPY; YERSINIA ENTEROCOLITICA;

ANTI-BACTERIAL AGENTS; BACILLUS; INDUSTRIAL WASTE; MICROBIAL SENSITIVITY TESTS; PEPTIDE HYDROLASES; SEAFOOD;

BACILLUS CEREUS; ESCHERICHIA COLI; LISTERIA MONOCYTOGENES; YERSINIA;

EID: 33947642676     PISSN: 09608524     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.biortech.2006.09.033     Document Type: Article

References (36)

1. Anustrup K. Proteinases. In: Rose A.H. (Ed). Economic Microbiology: Microbial Enzymes and Bioconversions vol. 5 (1980), Academic Press, New York 50-114
2. Cappuccino J.G., and Sherman N. Microbiology: A Laboratory Manual (2004), Pearson Education, Singapore p. 491
3. Chakraborty R., and Srinivasan M. Production of a thermostable alkaline protease by a new Pseudomonas sp. by solid substrate fermentation. Journal of Microbiology and Biotechnology 8 (1993) 7-16
4. Chong A.S.C., Hashim R., Chow-Yang L., and Ali A.B. Partial characterization and activities of proteases from digestive tract of discus fish (Symphysodon aequifasciata). Aquaculture 203 (2002) 321-333
5. Chrost R.J. Microbial ectoenzymes in aquatic environments. In: Overbeck J., and Chrost R.J. (Eds). Aquatic Microbial Ecology (1990), Springer, New York 47-78
6. Ferrari E., Jarnagin A.S., and Schmit B.F. Commercial production of extracellular enzymes. In: Sonenshein A.L., Hoch J.A., and Losick R. (Eds). Bacillus subtilis and Other Gram-positive Bacteria (1993), American Society for Microbiology, Washington DC 917-937
7. Ferrero M.A., Castro G.R., Abate C.M., Baigori M.D., and Sineriz F. Thermo stable alkaline protease of Bacillus licheniformis MIR29 : isolation, production and characterization. Applied Microbiology and Biotechnology 45 (1996) 327-332
8. Fox J.W., Shannon J.D., and Bjarnason J.B. Proteinases and their Inhibitors in Biotechnology. Enzymes in Biomass Conversion. ACS Symposium Series 460 (1991) 62-79
9. George S., Raju V., Krishnan M.R.V., Subramanian T.V., and Jayaraman K. Production of protease by Bacillus amyloliquefaciens in solid-state fermentation and its application in the unhairing of hides and skins. Process Biochemistry 30 (1995) 457-462
10. Godfrey T., and West S. Industrial Enzymology (1996), Macmillan Publishers Inc., New York, NY pp. 3-10
11. Gupta R., Beg Q.K., and Lorenz P. Bacterial alkaline proteases; molecular approaches and industrial applications. Applied Microbiology and Biotechnology 59 (2002) 15-32
12. Kobayashi T., Hakamada Y., Adachi S., Hitomi J., Yoshimatsu T., Koike K., Kawai S., and Ito S. Purification and properties of an alkaline protease from alkalophilic Bacillus sp. KSM-K16. Applied Microbiology and Biotechnology 43 (1995) 473-481
13. Kim Y.K., Bae J.H., Oh B.K., Lee W.H., and Choi J.W. Enhancement of proteolytic enzyme activity excreted from Bacillus stearothermophilus for a thermophilic aerobic digestion process. Bioresource Technology 82 (2002) 157-164
14. Kwon Y.T., Kim J.O., Moon S.Y., Lee H.H., and Rho H.M. Extracellular alkaline protease from alkalophilic Vibrio metschnikovii strain RH530. Biotechnology Letters 16 (1994) 413-418
15. 4. Nature 227 (1970) 680-685
16. Lowry O.H., Rosenborough N.J., Farr A.L., and Randall R.J. Protein measurement with the folin-phenol reagent. Journal of Biological Chemistry 193 (1951) 265-275
17. Mahendrakar N.S. Aquafeeds and meat quality of cultured fish. In: John G., and Ninawe A.S. (Eds). Aquaculture - Feed and Health (2000), Biotechnology Consortium India Ltd., New Delhi 26-30
18. McDougall L.A., Holzafel W.H., Schillinger U., Freely D.E., and Rupnow J.H. Scanning electron microscopy of target cells and molecular weight determination of bacteriocin produced by Lactococcus lactis D53. International Journal of Food Microbiology 24 (1994) 295-308
19. Moon H., and Parulekar S.J. A parametric study of protease production in batch and fed-batch cultures of Bacillus firmus. Biotechnology and Bioengineering 37 (1991) 467-483
20. Moriarty D.J.W. Control of luminous Vibrio species in penaeid aquaculture ponds. Aquaculture 164 (1998) 351-358
21. Najafi M.F., Deobagkar D., and Deobagkar D. Potential application of protease isolated from Pseudomonas aeruginosa PD100. Electronic Journal of Biotechnology 8 (2005) 198-207
22. Ochoa S.J.L., and Olmos S.J. The functional property of Bacillus for shrimp feeds. Food Microbiology 23 (2006) 519-525
23. Sephton, S.W., Swan, J.E. and Krzyzosiak, J. 1996. Enzymes in meat processing effect of liver hydrolysates on sausage cook yields and cat food acceptability. In: Publications, Meat Industry Research Institute of New Zealand, 1995, No. 954, iv. p. 13.
24. Rao M.B., Tanksale A.M., Ghatge M.S., and Deshpande V.V. Molecular and biotechnological aspects of microbial proteases. Microbiology and Molecular Biology Reviews 62 (1998) 597-635
25. Rebecca B.D., Pena-Vera M.T., and Diaz-Castaneda M. Production of fish protein hydrolysates with bacterial protease; yield and nutritional value. Journal of Food Science 56 (1991) 309-314
26. Rengipat S., Phianphak W., Piyatirativorakul S., and Menasveta P. Effects of a probiotic bacterium on black tiger shrimp Penaeus monodon survival and growth. Aquaculture 167 (1998) 310-313
27. Samal B.B., Karan B., Parker C., and Stabinsky Y. Isolation and thermal stability studies of two novel serine proteinases from the fungus Tritirachium album limber. Enzyme and Microbial Technology 13 (1991) 66-70
28. Sangbrita S., Roy R.N., Sen S.K., and Ray A.K. Characterisation of cellulose-producing bacteria from the digestive tract of tilapia, Oreochromis mossambica (Peters) and grass carp, Ctenopharyngodon idella (Valenciennes). Aquaculture Research 37 (2006) 380-388
29. Strauch M.A., and Hoch J.A. Transition-state regulators: sentinels of Bacillus subtilis post-exponential phase gene expression. Molecular Microbiology 7 (1993) 337-342
30. Sudeepa, E.S., Rashmi, H.N., Tamilselvi, A., Bhaskar, N., 2007. Proteolytic bacteria associated with fish processing wastes: isolation and characterization. Journal of Food Science and Technology 44, in press.
31. Sugita H., Kawasahi J., and Deguchi Y. Production of amylase by the intestinal microflora in cultured fresh water fish. Letters in Applied Microbiology 24 (1997) 105-108
32. Sugita H., Shibuya K., Shimooka H., and Deguchi Y. Antibacterial abilities of intestinal bacteria in fresh water cultured fish. Aquaculture 145 (1996) 195-203
33. Tanimoto S., Tanabe S., Watanabe M., and Arai S. Enzymatic modification of Zein to produce a non-bitter peptide fraction with a very high fischer ratio for patients with hepatic encephalopathy. Agricultural and Biological Chemistry 55 (1991) 1119-1123
34. Tsujibo H., Miyamoto K., Hasegawa T., and Inamori Y. Purification and characterization of two types of alkaline serine proteases produced by an alkalophilic actinomycete. Journal of Applied Bacteriology 69 (1990) 520-529
35. Verschuere L., Romabut G., Sorgeloos P., and Verstraete W. Probiotic bacteria as biological control agents in aquaculture. Microbiology and Molecular Biology Reviews 64 (2000) 655-671
36. Vinci V.A., Aphale J.S., Gibb G.D., and Strow W.R. Purification and properties of the chymotrypsin like serine proteinase over produced by Streptomyces sp. strain C5-A13. Applied Microbiology and Biotechnology 39 (1993) 69-73