Engineering of the α-amylase from geobacillus stearothermophilus US100 for detergent incorporation

Biotechnology and Bioengineering

Volumn 102, Issue 2, 2009, Pages 380-389

  Khemakhem, Bassem ^a,b   Ali, Mamdouh Ben ^a   Aghajari, Nushin ^b   Juy, Michel ^b   Haser, Richard ^b   Bejar, Samir ^a  

^a CENTRE DE BIOTECHNOLOGIE DE SFAX   (Tunisia)

^b INSTITUT DE BIOLOGIE ET CHIMIE DES PROTÉINES   (France)

Author keywords

Amylase; Detergents; Geobacillus stearothermophilus; Mutagenesis; Oxidation; Thermostable

Indexed keywords

CHEMICAL OXIDATIONS; DETERGENT INDUSTRIES; GEOBACILLUS STEAROTHERMOPHILUS; HIGH RESISTANCES; MALTOHEXAOSE; POTENTIAL APPLICATIONS; SPECIFIC ACTIVITIES; STARCH HYDROLYSIS; STRUCTURAL STUDIES; THERMOSTABLE; WILD-TYPE ENZYMES;

DETERGENTS; ENZYMES; MUTAGENESIS; OXIDATION;

SOAPS (DETERGENTS);

ALANINE; AMYLASE; DETERGENT; METHIONINE; MALTOHEXAOSE; OLIGOSACCHARIDE; UNCLASSIFIED DRUG;

AMINO ACID SUBSTITUTION; ARTICLE; BACTERIAL STRAIN; BIOENGINEERING; CONTROLLED STUDY; GEOBACILLUS STEAROTHERMOPHILUS; NONHUMAN; BACILLUS; BIOSYNTHESIS; CATALYSIS; CHEMISTRY; ENZYME STABILITY; ENZYMOLOGY; ESCHERICHIA COLI; GENETICS; HEAT; MUTAGENESIS; OXIDATION REDUCTION REACTION; PROTEIN CONFORMATION; PROTEIN ENGINEERING; STRUCTURE ACTIVITY RELATION;

GEOBACILLUS STEAROTHERMOPHILUS;

ALANINE; ALPHA-AMYLASES; AMINO ACID SUBSTITUTION; BACILLUS; CATALYSIS; DETERGENTS; ENZYME STABILITY; ESCHERICHIA COLI; HOT TEMPERATURE; METHIONINE; MUTAGENESIS; OLIGOSACCHARIDES; OXIDATION-REDUCTION; PROTEIN CONFORMATION; PROTEIN ENGINEERING; STRUCTURE-ACTIVITY RELATIONSHIP;

EID: 58249088353     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.22083     Document Type: Article

References (45)

1. Aehle W. 1997. Development of new amylases. In: van Ee JH, Misset O, Baas EJ, editors. Enzymes in detergency. New York, NY: Marcel Dekker, Inc. pp. 213-229.
2. Aghajari N, Roth M, Haser R. 2002. Crystallographic evidence of a transglycosylation reaction: Ternary complexes of a psychrophilic α-amylase. Biochemistry 41:4273-280.
3. Aronson MP, Cardinali MS, McCown JT. 1990. Stabilized enzymes liquid detergent composition containing lipase and protease. US Patent 4959179. http://www.freepatentsonline.com/4959179.html.
4. Ausubel FM, Brent R, Kingston RE, Moore DD, Seidman JG, Smith JA, Struhl K. 1993. Current Protocols in Molecular Biology. New York: Greene Publishing Associates and Wiley.
5. Ben Ali M, Mezghani M, Bejar S. 1999. A thermostable [alpha]-amylase producing maltohexaose from a new isolated Bacillus sp. US100: Study of activity and molecular cloning of the corresponding gene. Enzyme Microb Technol 24:584-589.
6. Ben Ali M, Mhiri S, Mezghani M, Bejar S. 2001. Purification and sequence analysis of the atypical maltohexaose-forming alpha-amylase of the B. stearothermophilus US100. Enzyme Microb Technol 28:537-542.
7. Ben Ali M, Khemakhem B, Robert X, Haser R, Bejar S. 2006. Thermostability enhancement and change in starch hydrolysis profile of the maltohexaose-forming amylase of Bacillus stearothermophilus US100 strain. Biochem J 394:51-56.
8. Bisgard-Frantzen HSA, Norman B, Pedersen S, Kjaeruff FS, Outtrup H, Borchert TV. 2000. Development of industrially important α-amylase. J Appl Glycosci 46:199-206.
9. Bradford MM. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248-254.
10. Brosnan MP, Kelly CT, Fogarty WM. 1992. Investigation of the mechanisms of irreversible thermoinactivation of Bacillus stearothermophilus alpha-amylase. Eur J Biochem/FEBS 203:225-231.
11. Bryan PN. 2000. Protein engineering of subtilisin. Biochim Biophys Acta (BBA) Protein Struct Mol Enzymol 1543(2):203-222.
12. Brzozowski AM, Lawson DM, Turkenburg JP, Bisgaard-Frantzen H, Svendsen A, Borchert TV, Dauter Z, Wilson KS, Davies GJ. 2000. Structural analysis ofa chimeric bacterial alpha-amylase. High-resolution analysis of native and ligand complexes. Biochemistry 39:9099-9107.
13. Buisson G, Duee E, Haser R, Payan F. 1987. Three dimensional structure of porcine pancreatic α-amylase at 2.9 Å resolution. Role of calcium in structure and activity. EMBO J 6:3909-3916.
14. Combet C, Jambon M, Deleage G, Geourjon C. 2002. Geno3d: Automatic comparative molecular modelling of protein. Bioinformatics 18:213-214.
15. Davies G, Henrissat B. 1995. Structures and mechanisms of glycosyl hydrolases. Structure 3:853-859.
16. Estell DA, Graycar TP, Wells JA. 1985. Engineering an enzyme by site-directed mutagenesis to be resistant to chemical oxidation. J Biol Chem 260:6518-6521.
17. Galante YM, Formantici C. 2003. Enzyme applications in detergency and in manufacturing industries. Curr Org Chem 7:1399-1422.
18. Gupta R, Beg QK, Lorenz P. 2002. Bacterial alkaline proteases: Molecular approaches and industrial applications. Appl Microbiol Biotechnol 59:15-32.
19. Guzman-Maldonado H, Paredes-Lopez O. 1995. Amylolytic enzymes and products derived from starch: A review. Crit Rev Food Sci Nutr 35:373-403.
20. Hatada Y, Masuda N, Akita M, Miyazaki M, Ohta Y, Horikoshi K. 2006. Oxidatively stable maltopentaose-producing [alpha]-amylase from a deep-sea Bacillus isolate, and mechanism of its oxidative stability validated by site-directed mutagenesis. Enzyme Microb Technol 39:1333-1340.
21. Igarashi K, Hatada Y, Ikawa K, Araki H, Ozawa T, Kobayashi T, Ozaki K, Ito S. 1998. Improved thermostability of a Bacillus alpha-amylase by deletion of an arginine-glycine residue is caused by enhanced calcium binding. Biochem Biophys Res Commun 248:372-377.
22. Igarashi K, Hagihara H, Ito S. 2003. Protein engineering of detergent α-amylases. Trends Glycosci Glycotechnol 82:101-114.
23. Ikram ul H, Ashraf H, Iqbal J, Qadeer MA. 2003. Production of alpha amylase by Bacillus licheniformis using an economical medium. Bio-resource Technol 87:57-61.
24. Ito S, Kobayashi T, Ara K, Ozaki K, Kawai S, Hatada Y. 1998. Alkaline detergent enzymes from alkaliphiles: Enzymatic properties, genetics, and structures. Extremophiles 2:185-190.
25. Janecek S. 2002. How many conserved sequence regions are there in the α-amylase family? Biologia (Bratislava) 57(Suppl. 11):29-41.
26. Kumar CG, Takagi H. 1999. Microbial alkaline proteases: From a bio-industrial viewpoint. Biotechnol Adv 17:561-594.
27. Laemmli UK, Beguin F, Gujer-Kellenberger G. 1970. A factor preventing the major head protein of bacteriophage T4 from random aggregation. J Mol Biol 47:69-74.
28. Machius M, Wiegand G, Huber R. 1995. Crystal structure of calcium-depleted Bacillus licheniformis [alpha]-amylase at 2.2 a resolution. J Mol Biol 246:545-559.
29. Machius M, Declerck N, Huber R, Wiegand G. 1998. Activation of Bacillus licheniformis alpha-amylase through a disorder-> order transition of the substrate-binding site mediated by a calcium-sodium-calcium metal triad. Structure 6(3):281-292.
30. Maurer KH. 2004. Detergent proteases. Curr Opin Biotechnol 15:330-334.
31. Miller GL. 1959. Use of dinitrosalicylic acid reagent for determination of reducing sugars. Anal Chem 31:426-428.
32. Mitidieri S, Souza Martinelli AH, Schrank A, Vainstein MH. 2006. Enzymatic detergent formulation containing amylase from Aspergillus niger: A comparative study with commercial detergent formulations. Bioresource Technol 97:1217-1224.
33. Nielsen JE, Borchert TV. 2000. Protein engineering of bacterial alpha-amylases. Biochim Biophys Acta 1543:253-274.
34. Qian M, Buisson G, Dute E, Haser H, Payan F. 1994. The active center of a mammalian α-amylase. Structure of the complex of a pancreatic a-amylase with a carbohydrate inhibitorr efined to 2.2 Å resolution. Biochemistry 33:6284-6294.
35. Ramachandran S, Patel AK, Nampoothiri KM, Francis F, Nagy V, Szakacs G, Pandey A. 2004. Coconut oil cake-A potential raw material for the production of alpha-amylase. Bioresource Technol 93:169-174.
36. Roussel A, Cambillau C. 1991. In silicon graphics geometry directory 86. Moutain View, CA: Silicon Graphics.
37. Sambrook J, Fritsch EF, Maniatis T. 1989. Molecular cloning: A laboratory manual. Cold Spring Harbor, New York: Cold Spring Harbor Laboratory.
38. Schroeder M, Lenting HBM, Kandelbauer A, Silva CJSM, Cavaco-Paulo A, Guebitz GM. 2006. Restricting detergent protease action to surface of protein fibres by chemical modification. Appl Microb Biotechnol 72:738-744.
39. Stoner MR, Dale DA, Gualfetti PJ, Becker T, Manning MC, Carpenter JF, Randolph TW. 2004. Protease autolysis in heavy-dutyliquid detergent formulations: Effects of thermodynamic stabilizers and protease inhibitors. Enzyme Microb Technol 34:114-125.
40. 2+ surfactant interactions affect enzyme stability in detergent solutions''. Biotechnol Prog 21(6):1716-1723.
41. Suvd D, Takase K, Fujimoto Z, Matsumura M, Mizuno H. 2000. Purification, crystallization and preliminary X-ray crystallographic study of alpha-amylase from Bacillus stearothermophilus. Acta Crystallogr 56:200-202.
42. Suvd D, Fujimoto Z, Takase K, Matsumura M, Mizuno H. 2001. Crystal structure of Bacillus stearothermophilus alpha-amylase: Possible factors determining the thermostability. J Biochem (Tokyo) 129(3):461-468.
43. Svensson B. 1994. Protein engineering in the alpha-amylase family: Catalytic mechanism, substrate specificity, and stability. Plant Mol Biol 25(2):141-157.
44. Svensson B, Tovborg Jensen M, Mori H, Sass Bak-Jensen K, Bønsager B, Nielsen PK, Birte Kramhøft B, Prætorius-Ibba M, Nøhr J, Juge N. 2002. Fascinating facets of function and structure of amylolytic enzymes of glycoside hydrolase family 13. Biologia (Bratislava) 57(Suppl. 11):5-19.
45. Vogt W. 1995. Oxidation of methionyl residues in proteins: Tools, targets, and reversal. Free Radic Biol Med 18(1):93-105.