Purification of alkaline proteases from a Bacillus strain and their possible interrelationship

Applied Microbiology and Biotechnology

Volumn 45, Issue 1-2, 1996, Pages 63-71

  Kobayashi, T ^a   Hakamada, Y ^a   Hitomi, J ^a   Koike, K ^a   Ito, S ^a  

^a KAO CORPORATION   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

ALKALINE PROTEINASE;

ARTICLE; BACILLUS; BACTERIAL GROWTH; CONTROLLED STUDY; ENZYME ANALYSIS; ENZYME PURIFICATION; ENZYME STABILITY; MOLECULAR CLONING; NONHUMAN; PROTEIN STRUCTURE;

AMINO ACID SEQUENCE; BACILLUS; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ENDOPEPTIDASES; ENZYME STABILITY; HYDROGEN-ION CONCENTRATION; ISOELECTRIC POINT; KINETICS; MOLECULAR SEQUENCE DATA; MOLECULAR WEIGHT; PROTEIN CONFORMATION; PROTEIN STRUCTURE, SECONDARY; SUBSTRATE SPECIFICITY; TEMPERATURE;

BACILLUS SP.; BACTERIA (MICROORGANISMS);

EID: 0030004496     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s002530050650     Document Type: Article

References (20)

1. Edelhoch H (1967) Spectroscopic determination of tryptophan and tyrosine in proteins. Biochemistry 6:1948-1954
2. Flatmark T (1964) On the heterogeneity of beef heart cytochrome c. I. Separation and isolation of subfractions by disc electrophoresis and column chromatography. Acta Chem Scand 18:1656-1666
3. Flatmark T (1966) On the heterogeneity of beef heart cytochrome c. II. Some physico-chemical properties of the main subfractions (Cyl CyIII). Acta Chem Scand 20:1476-1486
4. Flatmark T. Sletten K (1968) Multiple forms of cytochrome c in rat. J Biol Chem 243:1623-1629
5. Funakoshi S, Deutsch HF (1969) Human carbonic anhydrases. J Biol Chem 244:3438-3446
6. Hakamada Y, Kobayashi T, Hitomi J, Kawai S, Ito S (1994) Molecular cloning and nucleotide sequence of the gene for an alkaline protease from the alkalophilic Bacillus sp. KSM-K16. J Ferment Bioeng 78:105-108
7. Hattori M, Ogawa H (1980) Characteristics of fibrous protein and the disulphide cross-linking band in human Stratum corneum. In: Bernstein IA, Seiji M (eds) Biochemistry of normal and abnormal epidermal differentiation. University of Tokyo Press. Tokyo, pp 379-390
8. Ichishima E, Maeba H, Amikura T, Sakata H (1984) Multiple forms of protyrosinase from Aspergillus oryzae and their mode of activation at pH 3.0. Biochim Biophys Acta 786:25-31
9. Ichishima E, Hanzawa S, Watanabe M, Takeuchi M (1986) Electrophoretic heterogeneity and aged-related change of serine proteinase from Aspergillus sojae. Curr Microbiol 13:231-235
10. Kobayashi T, Hakamada Y, Adachi S, Hitomi J, Yoshimatsu T, Koike K, Kawai S, Ito S (1995) Purification and properties of an alkaline protease from alkalophilic Bacillus sp KSM-K16. Appl Microbiol Biotechnol 43:473-481
11. Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680-685
12. Lowry OH, Rosebrough NJ, Farr AL, Randall JR (1951) Protein measurement with the folin phenol reagent. J Biol Chem 193:265-275
13. Makino K, Ogaki J, Nishihara T, Ichikawa T, Kondo M (1983) Studies on protease from marine bacteria 2. Properties of extra-cellular protease from marine Pseudomonas sp 145-2. Microbios 36:7-20
14. Taber HW, Sherman F (1964) Spectropholometric analyzers for disk electrophoresis; studies of yeast cytochrome c. Ann NY Acad Sci 121:600-615
15. Towbin H, Staehelin T, Gordon J (1979) Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedures and some applications. Proc Natl Acad Sci USA 76:4350-4354
16. Yamagata H, Tanaka K, Kasai Z (1979) Isoenzymes of acid phosphatase in aleurone particles of rice grains and their interconversion. Agric Biol Chem 43:2059-2066
17. Yamane T, Kani T, Hatanaka T, Suzuki A, Ashida T, Kobayashi T, Ito S, Yamashita O (1995) Structure of a new alkaline serine protease (M-protease) from Bacillus sp KSM-K16. Acta Crystallogr D 51:199-206
18. Yang JT, Wu CSC, Martinez HM (1986) Calculation of protein conformation from circular dichroism. Methods Enzymol 130:208-269
19. Yoshida K, Hidaka H, Miyado S, Shibata U, Saito K, Yamada Y (1977) Purification and some properties of Bacillus sphaericus protease. Agric Biol Chem 41:745-754
20. Zuidweg MHJ, Bos CJK, Van Weizen H (1972) Proteolytic components of alkaline proteases of Bacillus strains. Zymograms and electrophoretic isolation. Biotechnol Bioeng 14:685-714