Purification, molecular cloning, and biochemical characterization of subtilisin JB1 from a newly isolated Bacillus subtilis JB1

Applied Biochemistry and Biotechnology

Volumn 162, Issue 3, 2010, Pages 900-911

  Sung, Ji Hea ^a   Ahn, Sang Jung ^a,b   Kim, Na Young ^a   Jeong, Soo Kyoung ^a   Kim, Joong Kyun ^a   Chung, Joon Ki ^a   Lee, Hyung Ho ^a  

^a Pukyong National University   (South Korea)

^b GEOPHYSICAL LABORATORY   (United States)

Author keywords

Bacillus subtilis JB1; Chemically assisted fragmentation (CAF); MALDI TOF MS; Peptide mass fingerprinting (PMF); Skim milk agar plate; Subtilisin

Indexed keywords

AGAR PLATES; BACILLUS SUBTILIS; CHEMICALLY ASSISTED FRAGMENTATION (CAF); MALDI TOF MS; SKIM MILKS; SUBTILISIN;

ALGAE; AMINO ACIDS; AMMONIUM COMPOUNDS; BACTERIOLOGY; BIODEGRADATION; BODY FLUIDS; CLONING; DEGRADATION; DESORPTION; ELECTROPHORESIS; ENZYME ACTIVITY; ENZYMES; ESCHERICHIA COLI; GEL PERMEATION CHROMATOGRAPHY; GELS; INDUCTIVELY COUPLED PLASMA; INDUSTRIAL APPLICATIONS; ION EXCHANGE; MASS SPECTROMETRY; MICROORGANISMS; PEPTIDES; PHOSPHATASES; POLYSACCHARIDES; PRECIPITATION (CHEMICAL); PURIFICATION; ROOM AND PILLAR MINING;

GENE ENCODING;

ARTICLE; BACILLUS SUBTILIS; BACTERIAL GENE; BIODEGRADATION; DNA PURIFICATION; ESCHERICHIA COLI; EXPRESSION VECTOR; GEL ELECTROPHORESIS; GEL FILTRATION CHROMATOGRAPHY; JB1 GENE; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; MOLECULAR CLONING; NONHUMAN;

BACILLUS SUBTILIS; BLOTTING, WESTERN; CHROMATOGRAPHY, GEL; CLONING, MOLECULAR; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ESCHERICHIA COLI; HYDROGEN-ION CONCENTRATION; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION; SUBTILISIN;

BACILLUS SUBTILIS; BOVINAE; ESCHERICHIA COLI;

EID: 77954887506     PISSN: 02732289     EISSN: None     Source Type: Journal    
DOI: 10.1007/s12010-009-8830-6     Document Type: Article

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