Dielectric spectroscopy-based estimation of VCD in CHO cell culture

Journal of Chemical Technology and Biotechnology

Volumn 90, Issue 2, 2015, Pages 273-282

  Lee, Hae Woo ^a   Carvell, John ^b   Brorson, Kurt ^c   Yoon, Seongkyu ^d  

^a Daegu Gyeongbuk Medical Innovation Foundation   (South Korea)

^b ABER INSTRUMENTS LTD   (United Kingdom)

^c CENTER FOR DRUG EVALUATION AND RESEARCH   (United States)

^d University of Massachusetts   (United States)

Author keywords

Chemometrics; Chinese hamster ovary (CHO) cells; Dielectric spectroscopy; Locally weighted partial least squares; Viable cell density

Indexed keywords

ANIMAL CELL CULTURE; CELL CULTURE; CELL GROWTH; CELLS; DIELECTRIC SPECTROSCOPY; DYNAMICS; GROWTH KINETICS; LEAST SQUARES APPROXIMATIONS; MAMMALS; MULTIVARIANT ANALYSIS; PHYSIOLOGY; REGRESSION ANALYSIS;

CHEMOMETRICS; CHINESE HAMSTER OVARY CELLS; ENVIRONMENTAL CONDITIONS; MAMMALIAN CELL CULTURE; MULTI VARIATE ANALYSIS; MULTIVARIATE REGRESSION MODELS; PARTIAL LEAST SQUARE (PLS); VIABLE CELLS;

CYTOLOGY;

ANIMAL CELL; ARTICLE; BIOREACTOR; CELL CULTURE; CELL DENSITY; CELL GROWTH; CELL VIABILITY; CHEMOMETRICS; CHO CELL LINE; COLE COLE EQUATION; CONTROLLED STUDY; ELECTROCHEMICAL IMPEDANCE SPECTROSCOPY; MATHEMATICAL MODEL; MULTIVARIATE ANALYSIS; NONHUMAN; PARTIAL LEAST SQUARES REGRESSION; PROTEIN SYNTHESIS; REGRESSION ANALYSIS;

CRICETULUS GRISEUS; MAMMALIA;

EID: 84920904066     PISSN: 02682575     EISSN: 10974660     Source Type: Journal    
DOI: 10.1002/jctb.4522     Document Type: Article

References (57)

1. Lubiniecki A, Historical reflections on cell culture engineering. Cytotechnology 28:139-145 (1998).
2. Wurm FM, Production of recombinant protein therapeutics in cultivated mammalian cells. Nature Biotechnol 22:1393-1398 (2004).
3. Andersen DC and Krummen L, Recombinant protein expression for therapeutic applications. Current Opin Biotechnol 13:117-123 (2002).
4. van der Valk J, Brunner D, De Smet K, Fex Svenningsen A, Honegger P, Knudsen LE, Lindl T, Noraberg J, Price A, Scarino ML and Gstraunthaler G, Optimization of chemically defined cell culture media - replacing fetal bovine serum in mammalian in vitro methods. Toxicol In Vitro 24:1053-1063 (2010).
5. Gunther JC, Conner JS and Seborg DE, Process monitoring and quality variable prediction utilizing PLS in industrial fed-batch cell culture. J Process Control 19:914-921 (2009).
6. Teixeira AP, Oliveira R, Alves PM and Carrondo MJT, Advances in on-line monitoring and control of mammalian cell cultures: supporting the PAT initiative. Biotechnol Adv 27:726-732 (2009).
7. Rathore AS, Yu M, Yeboah S and Sharma A, Case study and application of process analytical technology (PAT) towards bioprocessing: use of on-line high-performance liquid chromatography (HPLC) for making real-time pooling decisions for process chromatography. Biotechnol Bioeng 100:306-316 (2008).
8. Abu-Absi NR, Kenty BM, Cuellar ME, Borys MC, Sakhamuri S, Strachan DJ, Hausladen MC and Li ZJ, Real time monitoring of multiple parameters in mammalian cell culture bioreactors using an in-line Raman spectroscopy probe. Biotechnol Bioeng 108:1215-1221 (2010).
9. Kamga M-H, Lee HW, Liu J and Yoon S, Rapid quantification of protein mixture in chromatographic separation using multi-wavelength UV spectra. Biotechnol Progress :doi: 10.1002/btpr.1712 (2013).
10. Lee HW, Bawn A and Yoon S, Reproducibility, complementary measure of predictability for robustness improvement of multivariate calibration models via variable selections. Analyt Chim Acta 757:11-18 (2012).
11. Lee HW, Christie A, Liu JJ and Yoon S, Estimation of raw material performance in mammalian cell culture using near infrared spectra combined with chemometrics approaches. Biotechnol Progress 28:824-832 (2012).
12. Lee HW, Christie A, Xu J and Yoon S, Data fusion-based assessment of raw materials in mammalian cell culture. Biotechnol Bioeng 109:2819-2828 (2012).
13. Read EK, Park JT, Shah RB, Riley BS, Brorson KA and Rathore AS, Process analytical technology (PAT) for biopharmaceutical products: Part I. concepts and applications. Biotechnol Bioeng 105:276-284 (2010).
14. Rathore AS, Bhambure R and Ghare V, Process analytical technology (PAT) for biopharmaceutical products. Analyt Bioanalyt Chem 398:137-154 (2010).
15. FDA, PAT-A framework for innovative pharmaceutical development, manufacturing and quality assurance. US Department of Health and Human Services, Food and Drug Administration, Center for Biologics Evaluation and Research, Rockville, MD (2004).
16. FDA, Guidance for industry. Process validation: general principles and practices. US Department of Health and Human Services, Food and Drug Administration, Center for Drug Evaluation and Research, Center for Biologics Evaluation and Research, Center for Veterinary Medicine, Rockville, MD (2011).
17. ICH, Guidance for Industry. Q8(R2) Pharmaceutical Development. US Department of Health and Human Services, Food and Drug Administration, Center for Biologics Evaluation and Research, Center for Biologics Evaluation and Research, Rockville, MD (2009).
18. Ansorge S, Esteban G and Schmid G, On-line monitoring of infected Sf-9 insect cell cultures by scanning permittivity measurements and comparison with off-line biovolume measurements. Cytotechnology 55:115-124 (2007).
19. Cannizzaro C, Gügerli R, Marison I and von Stockar U, On-line biomass monitoring of CHO perfusion culture with scanning dielectric spectroscopy. Biotechnol Bioeng 84:597-610 (2003).
20. Carvell J and Dowd J, On-line measurements and control of viable cell density in cell culture manufacturing processes using radio-frequency impedance. Cytotechnology 50:35-48 (2006).
21. Crowley J, Arnold SA, Wood N, Harvey LM and McNeil B, Monitoring a high cell density recombinant Pichia pastoris fed-batch bioprocess using transmission and reflectance near infrared spectroscopy. Enzyme Microbial Technol 36:621-628 (2005).
22. Finn B, Harvey LM and McNeil B, Near-infrared spectroscopic monitoring of biomass, glucose, ethanol and protein content in a high cell density baker's yeast fed-batch bioprocess. Yeast 23:507-517 (2006).
23. Li B, Ryan PW, Ray BH, Leister KJ, Sirimuthu NMS and Ryder AG, Rapid characterization and quality control of complex cell culture media solutions using raman spectroscopy and chemometrics. Biotechnol Bioeng 107:290-301 (2010).
24. Bittner C, Wehnert G and Scheper T, In situ microscopy for on-line determination of biomass. Biotechnol Bioeng 60:24-35 (1998).
25. Guez JS, Cassar JP, Wartelle F, Dhulster P and Suhr H, Real time in situ microscopy for animal cell-concentration monitoring during high density culture in bioreactor. J Biotechnol 111:335-343 (2004).
26. Wiedemann P, Guez JS, Wiegemann HB, Egner F, Quintana JC, Asanza-Maldonado D, Filipaki M, Wilkesman J, Schwiebert C, Cassar JP, Dhulster P and Suhr H, In situ microscopic cytometry enables noninvasive viability assessment of animal cells by measuring entropy states. Biotechnol Bioeng 108:2884-2893 (2011).
27. Kilburn DG, Fitzpatrick P, Blake-Coleman BC, Clarke DJ and Griffiths JB, On-line monitoring of cell mass in mammalian cell cultures by acoustic densitometry. Biotechnol Bioeng 33:1379-1384 (1989).
28. Dorresteijn RC, Numan KH, de Gooijer CD, Tramper J and Beuvery EC, On-line estimation of the biomass activity during animal-cell cultivations. Biotechnol Bioeng 51:206-214 (1996).
29. Kiviharju K, Salonen K, Moilanen U and Eerikäinen T, Biomass measurement online: the performance of in situ measurements and software sensors. J Ind Microbiol Biotechnol 35:657-665 (2008).
30. Justice C, Brix A, Freimark D, Kraume M, Pfromm P, Eichenmueller B and Czermak P, Process control in cell culture technology using dielectric spectroscopy. Biotechnol Adv 29:391-401 (2011).
31. Cole KS and Cole RH, Dispersion and absorption in Dielectrics I. Alternating current characteristics. J Chem Phys 9:341-351 (1941).
32. Harris CM, Tood RW and Bungard SJ, Dielectric permittivity of microbial suspensions at radio frequencies: a novel method for the real-time estimation of microbial biomass. Enzyme Microbial Technol 9:181-186 (1987).
33. Schwan HP, Electrical properties of tissue and cell suspensions. Adv Biol Med Phys 5:147-209 (1957).
34. Dabros M, Dennewald D, Currie DJ, Lee MH, Todd RW, Marison IW and Von Stockar U, Cole-Cole, linear and multivariate modeling of capacitance data for on-line monitoring of biomass. Bioprocess Biosyst Eng 32:161-173 (2009).
35. Kiviharju K, Salonen K, Moilanen U and Eerikäinen T, Biomass measurement online: the performance of in situ measurements and software sensors. J Ind Microbiol Biotechnol 35:657-665 (2008).
36. Soley A, Lecina M, Gamez X, Cairo JJ, Riu P, Rosell X, Bragos R and Godia F, On-line monitoring of yeast cell growth by impedance spectroscopy. J Biotechnol 118:398-405 (2005).
37. Ducommun P, Kadouri A, von Stockar U and Marison IW, On-line determination of animal cell concentration in two industrial high-density culture processes by dielectric spectroscopy. Biotechnol Bioeng 77:316-323 (2002).
38. Ansorge S, Esteban G and Schmid G, On-line monitoring of responses to nutrient feed additions by multi-frequency permittivity measurements in fed-batch cultivations of CHO cells. Cytotechnology 62:121-132 (2010).
39. Opel CF, Li J and Amanullah A, Quantitative modeling of viable cell density, cell size, intracellular conductivity, and membrane capacitance in batch and fed-batch CHO processes using dielectric spectroscopy. Biotechnol Prog 26:1187-1199 (2010).
40. Markx GH, ten Hoopen HJG, Meijer JJ and Vinke KL, Dielectric spectroscopy as a novel and convenient tool for the study of the shear sensitivity of plant cells in suspension culture. J Biotechnol 19:145-157 (1991).
41. Logan D and Carvell J, A biomass monitor for disposable bioreactors. Bioprocess Int 9:48-54 (2011).
42. Parta L, Zalai D, Borbely S and Putics A, Application of dielectric spectroscopy for monitoring high cell density in monoclonal antibody producing CHO cell cultivations. Bioprocess Biosyst Eng 37(2): 311-323 (2014).
43. Diagnostics R, Cedex Analyzer Operator's Guide. Roche Diagnostics, Mannheim, Germany (2011).
44. Wold S, Esbensen K and Geladi P, Principal component analysis. Chemomet Intell Lab Syst 2:37-52 (1987).
45. Maeder M, Evolving factor analysis for the resolution of overlapping chromatographic peaks. Analyt Chem 59:527-530 (1987).
46. Erriksson L, Johansson E, Kettaneh-Wold N, Trygg J, Wikstrom C and Wold S, Multi- and Megavariate Data Analysis. Umetrics AB, Umea, Sweden (2006).
47. Keller HR and Massart DL, Evolving factor analysis. Chemometrics Intell Lab Syst 12:209-224 (1992).
48. TM. Eigenvector Research, Inc., Wenatchee, WA (2006).
49. Geladi P and Kowalski BR, Partial least-squares regression: a tutorial. Analytica Chim Acta 185:1-17 (1986).
50. Rosipal R and Trejo LJ, Kernel partial least squares regression in reproducing kernel hilber space. J Machine Learning Res 2:97-123 (2001).
51. Centner V and Massart DL, Optimization in locally weighted regression. Anal Chem 70:4206-4211 (1998).
52. Naes T, Isaksson T and Kowalski B, Locally weighted regression and scatter correction for near-infrared reflectance data. Anal Chem 62:664-673 (1990).
53. Hoffmann H, Schaal S and Vijayakumar S, Local dimensionality reduction for non-parametric regression. Neural Proc Lett 29:109-131 (2009).
54. Chisti Y, Animal-cell damage in sparged bioreactors. Trends Biotechnol 18:420-432 (2000).
55. Ansorge S, Esteban G and Schmid G, Multifrequency permittivity measurements enable on-line monitoring of changes in intracellular conductivity due to nutrient limitations during batch cultivations of CHO cells. Biotechnol Prog 26:272-283 (2009).
56. Carvell J, Insights into Monitoring Changes in the Viable Cell Density and Cell Physiology using Scanning, Multi-frequency Dielectric Spectroscopy. IFPAC 2013, Baltimore, MD (2013).
57. Warikoo V, Godawat R, Brower K, Jain S, Cummings D, Simons E, Johnson T, Walther J, Yu M, Wright B, McLarty J, Karey KP, Hwang C, Zhou W, Riske F and Konstantinov K, Integrated continuous production of recombinant therapeutic proteins. Biotechnol Bioeng 109:3018-3029 (2012).