On-line measurements and control of viable cell density in cell culture manufacturing processes using radio-frequency impedance

Cytotechnology

Volumn 50, Issue 1-3, 2006, Pages 35-48

  Carvell, John P ^a   Dowd, Jason E ^b  

^a ABER INSTRUMENTS LTD   (United Kingdom)

^b JEHD Consulting   (Canada)

Author keywords

Capacitance; Cell culture; cGMP; CHO; On line biomass monitoring; Perfusion culture; Radio frequency impedance

Indexed keywords

CYCLIC GMP; RECOMBINANT PROTEIN;

ANIMAL CELL; BIOREACTOR; CELL COUNT; CELL CULTURE; CELL DENSITY; CELL SUSPENSION; HUMAN; HUMAN CELL; IMMOBILIZED CELL; MICROCARRIER CULTURE; NONHUMAN; ONLINE SYSTEM; RADIOFREQUENCY; REVIEW;

ANIMALIA;

EID: 33747337529     PISSN: 09209069     EISSN: 15730778     Source Type: Journal    
DOI: 10.1007/s10616-005-3974-x     Document Type: Review

References (42)

1. Asami K., Yonezawa T., Wakamatsu H. and Koyanagi N. 1996. Dielectric spectroscopy of biological cells. Biochem. Bioener. 40: 141-145.
2. Barer M.R., Kaprelyants A.S., Weichart D.H., Harwood C.R. and Kell D.B. 1998. Microbial stress and culturability: conceptual and operational domains. Microbiology UK 144: 2009-2010.
3. Belving H., Ericksson L.E.G., Davey C.L. and Kell D.B. 1994. Dielectric properties of human blood and erythrocytes at radio frequencies (0.2-10 MHz); dependence on cell volume fraction and medium composition. Eur. Biophys. J. 23: 207-215.
4. Carvell J.P. 2003. Monitoring live cell concentration in real time. Bioprocess Int.: 2-7.
5. Cerkel I., Garcia A., Degouys V., Dubois D., Fabry L. and Miller A.O.A. 1993. Dielectric-spectroscopy of mammalian cells: evaluation of the biomass of Hela-Cell and CHO-cells in suspension by low frequency dielectric spectroscopy. Cytotechnology 13: 185-193.
6. Clegg J.S. 1984. Properties and metabolism of the aqueous cytoplasmand its boundaries. Am. J. Physiol. 246:R133-R151.
7. Davey C.L. and Kell D.B. 1995. The low-frequency dielectric properties of biological cells. In: Bioelectrochemistry: Principles and Practice, Vol. 2. Bioelectrochem' of Cells and Tissues. pp. 159-207.
8. Davey C.L., Davey H.M., Kell D.B. and Todd R.W. 1993a. Introduction to the dielectric estimation of cellular biomass in real time, with special emphasis on measurements at high volume fractions. Anal. Chim. Acta 279: 155-161.
9. Davey C.L., Markx G.H. and Kell D.B. 1993b. On the dielectric method of measuring cellular viability. Pure Appl. Chem. 65: 1921-1926.
10. Davey C.L., Guan Y. and Kemp R.B. 1997a. Real time monitoring of the biomass content of animal cell cultures using dielectric spectroscopy. Animal Cell Technol.: Basic Appl. Aspects 8: 61-65.
11. Davey C.L., Guan Y., Kemp R.B. and Kell D.B. 1997b. Realtime monitoring of the biomass content of animal cell cultures using dielectric spectroscopy. In: Funatsu K., Shirai Y. and Matsushita T. (eds), Animal Cell Technology: Basic and Applied Aspects, Vol. 8. Kluwer, Dordrecht, pp. 61-65.
12. Degouys V., Cerkel I., Garcia A., Harfield J., Dubois D., Fabry L. and Miller A.O.A. 1993. Dielectric spectroscopy of mammalian cells: 2, simultaneous in situ evaluation by aperture impedance pulse spectroscopy and low-frequency dielectric spectroscopy of the biomass of HTC cells on Cytodex 3. Cytotechnology 13: 195-202.
13. Dowd J.E., Jubb A., Kwok E.K. and Piret J.M. 2003. Optimization and control of perfusion cultures using a viable cell probe and cell specific perfusion rates. Cytotechnology 42: 35-45.
14. Dowd J.E. and Carvell J.P. 2005. Improved control of cGMP fermentations and cell culture. Genet. Engineer. News 25(11): 64-68.
15. Ducommun P., Kadori A., Von Stockar U. and Marison I. 2002a. On-line determination of animal cell concentration in two industrial high density culture processes by dielectric spectroscopy. Biotechnol. Bioeng. 77: 316-323.
16. Ducommun P., Ruffieux P.A., Kadouri A., Von Stockar U. and Marison 2002b. Monitoring temperature effects on cell metabolism in a packed bed process. Biotechnol. Bioeng. 77: 838-842.
17. 7 cells per ml and production of B-Galactosidase at high cell density by fed batch culture. Biotechnol. Bioeng. 68: 381-388.
18. Elias C.B., Zeiser A. and Kamen A.A. 2003. Advances in high cell density culture technology using the Sf-9 insect cell baculovirus expression system - the fed batch approach. Bioprocess J. 2(1): 22-29.
19. Ferreira A.P., Vieira L.M., Cordoso J.P. and Menzes J.C. 2005. Evaluation of a new capacitance probe for biomass monitoring in industrial pilot-scale fermentations. J. Biotechnol. 116: 403-409.
20. Foster K.R. and Schwan H.P. 1986. Dielectric properties of tissues. In: Polk C. and Postow E. (eds), CRC Handbook of Biological Effects of Electromagnetuic Fields. CRC Press, Boca Raton, FL.
21. Foster K.R. and Schwan H.P. 1989. Dielectric properties of tissues and biological materials: a critical review. Critical Reviews in Biomedical Engineering 17: 25-104.
22. Guan Y. and Kemp R. 1997. The viable cell monitor: a dielectric spectroscope for growth and metabolic studies of animal cells on macroporous beads. In: Merten O.-W., Perrin P. and Griffiths B. (eds), New Developments and New Applications in Animal Cell Technology. Kluwer Academic Publishers, Dordrecht/NL, pp. 321-328.
23. Guan Y., Evans P.M. and Kemp R.B. 1998. An on-line monitor and potential control variable of specific metabolic rate in animal cell culture that combines microcalorimetry with dielectric spectroscopy. Biotechnol. Bioeng. 58: 463-477.
24. Harris C.M., Todd R.W., Bungard S.H., Lovitt R.W., Morris J.G. and Kell D.B. 1987. The dielectric permietivity of microbial suspensions at radio frequencies: a novel method for the estimation of microbial biomass. Enzyme Microb. Technol. 9: 181-186.
25. Kell D.B. and Todd R.W. 1998. Dielectric estimation of microbial biomass using the Aber Instruments Biomass Monitor. TIBTECH 16: 149-150.
26. Kell D.B., Markx G.H., Davey C.L. and Todd R.W. 1990. Real time monitoring of cellular biomass: methods and applications. Trends Anal. Chem. 9: 190-194.
27. Kell D.B., Kaprelyants A.S., Weichart D.H., Harwood C.L. and Baxter M.R. 1998. Viability and activity in readily culturable bacteria: a review and discussion of the practical issues. Antonie van Leeuwenhoek 73: 169-187.
28. Konstantinov K.B., Pambayun R., Matanguihan R., Yoshida T.Perusich C.M. and Hu W.S. 1992. On-line monitoring of hybridoma cell growth using a laser turbidity sensor. Biotechnol. Bioeng. 40: 1337-1342.
29. Konstantinov K., Chuppa S., Saja E., Tsal Y., Yoons and Golini F. 1994. Real time biomass concentration monitoring in animal cell cultures. TIBTECH 12: 324-333.
30. Merten O.-W., Palfi G.E., Stäheli J. and Steiner J. 1987. Invasive infrared sensor for the determination of the cell number in a continuous fermentation of hybridomas. Dev. Biol. Standard 66: 357-360.
31. Noll T. and Biselli M. 1998. Dielectric spectroscopy in the cultivation of suspended and immobilised hybridoma cells. J. Biotechnol. 63: 187-198.
32. Pethig R. 1979. Dielectric and Electronic Properties of Biological Materials. Wiley, Chichester.
33. Pethig R. and Kell D.B. 1987. The passive electrical properties of biological systems: their significance in physiology, biophysics and biotechnology. Phys. Med. Biol. 32: 933-970.
34. Schmid G. and Zacher D. 2004. Evaluation of a novel capacitance probe for on-line monitoring of viable cell densities in batch and fed-bach animal cell culture processes. In: Godia F. and Fussenegger M. (eds), Animal Cell Technology Meets Genomics. Springer, Dordrecht/NL, pp. 621-624.
35. Siano S.A. 1997. Biomass measurement by inductive permeattivity. Biotechnol. Bioeng. 55: 289-304.
36. Stoicheva N.G., Davey C.L., Markx G.H. and Kell D.B. 1989. Dielectric spectroscopy: a rapid method for the determination of solvent biocompatibility during biotransformations. Biocatalysis 2: 5-22.
37. Takashima S., Asami K. and Takahashi Y. 1988. Frequency domain studies of impedance characteristics of biological cells using micropippette technique. 1. Erythrocyte. Biophys. J. 54: 995-1000.
38. Vits H. and Hu W.S. 1992. Fluctuations in continuous mammalian cell bioreactors with retention. Biotechnol. Progr. 8: 397-403.
39. Wu P., Ozturk S., Blackie J.D., Thrift J.C., Figueroa C. and Naveh D. 1995. Evaluation and applications of optical density probes in mammalian cell bioreactors. Biotechnol. Bioeng. 45: 495-502.
40. Zeiser A., Bedard C., Voyer R., Jardin B., Tom R., Karmen A.A. and Karmen T. 1999. On-line monitoring of the progress of infection in Sf-9 insect cell cultures using relative permittivity measurements. Biotechnol. Bioeng. 63: 122-126.
41. Zeiser A., Voyer R., Jardin B. and Kamen A. 2000. On-line monitoring physiological parameters of insect cell cultures during growth and infection process. Biotechnol. Progr. 16: 803-808.
42. Zho W. and Hu W.S. 1994. On-line characterisation of a hybridoma cell culture process. Biotechnol. Bioeng. 44: 170-177.