Multifrequency permittivity measurements enable on-line monitoring of changes in intracellular conductivity due to nutrient limitations during batch cultivations of CHO cells

Biotechnology Progress

Volumn 26, Issue 1, 2010, Pages 272-283

  Ansorge, Sven ^a   Esteban, Geoffrey ^b   Schmid, Georg ^a  

^a F HOFFMANN LA ROCHE LTD   (Switzerland)

^b Fogale Nanotech   (France)

Author keywords

CHO cell cultures; Metabolic shift; On line monitoring; Permittivity; Physiological state

Indexed keywords

BATCH CULTIVATION; BIOVOLUMES; CELL SIZE; CHARACTERISTIC FREQUENCIES; CHO CELL; DISPERSION PARAMETERS; DUAL FREQUENCY; EXPONENTIAL GROWTH; HOST CELLS; IN-CELL; IN-PROCESS; MAMMALIAN CELL CULTURE; MEMBRANE AREA; METABOLIC SHIFT; MULTI FREQUENCY; NUTRIENT AVAILABILITY; NUTRIENT LIMITATIONS; ONLINE MONITORING; PHYSIOLOGICAL STATE; PILOT SCALE; PROCESS DATA; REDUCED GROWTH RATE; SUDDEN CHANGE; VIABLE CELLS;

AMINO ACIDS; CELLS; DISPERSIONS; MAMMALS; MEASUREMENTS; METABOLISM; NUTRIENTS; PERMITTIVITY; PERMITTIVITY MEASUREMENT; PROCESS MONITORING; VOLTAGE MEASUREMENT;

CELL CULTURE;

ANIMAL; ARTICLE; BIOREACTOR; CHO CELL; CRICETULUS; CULTURE TECHNIQUE; ELECTRIC CONDUCTIVITY; HAMSTER; METHODOLOGY; ONLINE SYSTEM;

ANIMALS; BIOREACTORS; CELL CULTURE TECHNIQUES; CHO CELLS; CRICETINAE; CRICETULUS; ELECTRIC CONDUCTIVITY; ONLINE SYSTEMS;

MAMMALIA;

EID: 76849104099     PISSN: 87567938     EISSN: 15206033     Source Type: Journal    
DOI: 10.1002/btpr.347     Document Type: Article

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