Quantitative modeling of viable cell density, cell size, intracellular conductivity, and membrane capacitance in batch and fed-batch CHO processes using dielectric spectroscopy

Biotechnology Progress

Volumn 26, Issue 4, 2010, Pages 1187-1199

  Opel, Cary F ^a   Li, Jincai ^a   Amanullah, Ashraf ^a  

^a GENENTECH INC   (United States)

Author keywords

Capacitance; Cole Cole equation; Dielectric spectroscopy; Online biomass monitoring; Partial least squares (PLS) regression; Permittivity

Indexed keywords

AMERICAN INSTITUTE OF CHEMICAL ENGINEERS; AUXILIARY INFORMATION; BIOMASS MEASUREMENTS; CELL CULTURE PROCESS; CELL RADIUS; CELL SIZE; CELL VOLUME; CHO CELL; COLE-COLE EQUATIONS; CRITICAL FREQUENCIES; DIELECTRIC ANALYSIS; DIELECTRIC PARAMETERS; DISTRIBUTION PARAMETERS; FED BATCHES; GROWTH PHASE; LINEAR MODEL; LINEAR MODELING; METHODS OF ANALYSIS; OFFLINE; ONLINE BIOMASS MONITORING; OXYGEN UPTAKE RATES; PARTIAL LEAST-SQUARES REGRESSION; PHYSIOLOGICAL STATE; QUANTITATIVE MODELING; SPECTROSCOPIC DATA; THREE MODELS; VIABLE CELLS;

BIOINFORMATICS; BIOMASS; CAPACITANCE; CELL CULTURE; DIELECTRIC DEVICES; DIELECTRIC SPECTROSCOPY; ECOLOGY; MONITORING; OXYGEN; PERMITTIVITY; PHYSIOLOGICAL MODELS; PHYSIOLOGY; REGRESSION ANALYSIS; SPECTROSCOPIC ANALYSIS; SPECTRUM ANALYSIS;

BATCH CELL CULTURE;

ANIMAL; ARTICLE; BIOMASS; CELL COUNT; CELL SIZE; CHO CELL; CRICETULUS; CULTURE TECHNIQUE; ELECTROCHEMICAL IMPEDANCE SPECTROSCOPY; HAMSTER; METHODOLOGY; REGRESSION ANALYSIS;

ANIMALS; BIOMASS; CELL COUNT; CELL CULTURE TECHNIQUES; CELL SIZE; CHO CELLS; CRICETINAE; CRICETULUS; DIELECTRIC SPECTROSCOPY; REGRESSION ANALYSIS;

EID: 77956970513     PISSN: 87567938     EISSN: 15206033     Source Type: Journal    
DOI: 10.1002/btpr.425     Document Type: Article

References (57)

1. Vojinovic V, Cabral JMS, Fonseca LP. Real-time bioprocess monitoring. I. In situ sensors. Sens Actuators B Chem. 2006; 114:1083-1091.
2. Kell DB, Markx GH, Davey CL, Todd RW. Real-time monitoring of cellular biomass: methods and applications. Trends Analyt Chem. 1990;9:190-194.
3. Kiviharju K, Salonen K, Moilanen U, Eerikäinen T. Biomass measurement online: the performance of in situ measurements and software sensors. J Ind Microbiol Biotechnol. 2008;35:657-665.
4. Höpfner T, Bluma A, Rudolph G, Lindner P, Scheper T. A review of non-invasive optical-based image analysis systems for continuous bioprocess monitoring. Bioprocess Biosyst Eng. 2010;33:247-256.
5. Junker BH, Reddy J, Gbewonyo K, Greasham R. On-line and in-situ monitoring technology for cell density measurement in microbial and animal cell cultures. Bioprocess Biosyst Eng. 1994;10:195-207.
6. Markx GH, Davey CL. The dielectric properties of biological cells at radiofrequencies: applications in biotechnology. Enzyme Microb Technol. 1999;25:161-171.
7. Asami K, Yonezawa T, Wakamatsu H, Koyanagi N. Dielectric spectroscopy of biological cells. Bioelectrochem Bioenerg. 1996;40:141-145.
8. Carvell J, Dowd J. On-line measurements and control of viable cell density in cell culture manufacturing processes using radiofrequency impedance. Cytotechnology. 2006;50:35-48.
9. Schwan HP. Electrical properties of tissue and cell suspensions. Adv Biol Med Phys. 1957;5:147-209.
10. Foster KR, Schwan HP. Dielectric properties of tissues and biological materials: a critical review. Crit Rev Biomed Eng. 1989; 17:25-104.
11. Cole KS, Cole RH. Dispersion and absorption in dielectrics. I. Alternating current characteristics. J Chem Phys. 1941;9:341-351.
12. Davey CL, Davey HM, Kell DB, Todd RW. Introduction to the dielectric estimation of cellular biomass in real time, with special emphasis on measurements at high volume fractions. Anal Chim Acta. 1993;279:155-161.
13. Pethig R, Kell DB. The passive electrical properties of biological systems: their significance in physiology, biophysics and biotechnology. Phys Med Biol. 1987;32:933-970.
14. Patel P, Markx GH. Dielectric measurement of cell death. Enzyme Microb Technol. 2008;43:463-470.
15. Patel PM, Bhat A, Markx GH. A comparative study of cell death using electrical capacitance measurements and dielectrophoresis. Enzyme Microb Technol. 2008;43:523-530.
16. Kell DB, Harris CM. Dielectric spectroscopy and membrane organisation. Electromagn Biol Med. 2009;4:317-348.
17. Markx GH, Davey CL, Kell DB. To what extent is the magnitude of the Cole-Cole a of the b-dielectric dispersion of cell suspensions explicable in terms of the cell size distribution? Bioelectrochem Bioenerg. 1991;25:195-211.
18. Neves AA, Pereira DA, Vieira LM, Menezes JC. Real time monitoring biomass concentration in Streptomyces clavuligerus cultivations with industrial media using a capacitance probe. J Biotechnol. 2000;84:45-52.
19. Arnoux AS, Preziosi-Belloy L, Esteban G, Teissier P, Ghommidh C. Lactic acid bacteria biomass monitoring in highly conductive media by permittivity measurements. Biotechnol Lett. 2005;27:1551-1557.
20. Fehrenbach R, Comberbach M, Pêtre JO. On-line biomass monitoring by capacitance measurement. J Biotechnol. 1992;23: 303-314.
21. Krairak S, Yamamura K, Nakajima M, Shimizu H, Shioya S. On-line monitoring of fungal cell concentration by dielectric spectroscopy. J Biotechnol. 1999;69:115-123.
22. Ciureanu M, Levadoux W, Goldstein S. Electrical impedance studies on a culture of a newly discovered strain of Streptomyces. Enzyme Microb Technol. 1997;21:441-449.
23. Cerckel I, Garcia A, Degouys V, Fabry L, Miller AOA. Dielectric spectroscopy of mammalian cells. 1. Evaluation of the biomass of HeLa- and CHO cells in suspension by low-frequency dielectric spectroscopy. Cytotechnology. 1993;13:185-193.
24. Degouys V, Cerckel I, Garcia A, Harfield J, Dubois D, Fabry L, Miller AOA. Dielectric spectroscopy of mammalian cells. II. Simultaneous in situ evaluation by aperture impedance pulse spectroscopy and low frequency dielectric spectroscopy of the biomass of HTC cells on Cytodex 3. Cytotechnology. 1993;13: 195-202.
25. Mas S, Ossard F, Ghommidh C. On-line determination of flocculating Saccharomyces cerevisiae concentration and growth rate using a capacitance probe. Biotechnol Lett. 2001;23:1125-1129.
26. Sarrafzadeh MH, Belloy L, Esteban G, Navarro JM, Ghommidh C. Dielectric monitoring of growth and sporulation of Bacillus thuringiensis. Biotechnol Lett. 2005;27:511-517.
27. Ducommun P, Kadouri A, von Stockar U, Marison IW. On-line determination of animal cell concentration in two industrial high-density culture processes by dielectric spectroscopy. Biotechnol Bioeng. 2002;77:316-323.
28. Ducommun P, Ruffieux PA, Kadouri A, von Stockar U, Marison IW. Monitoring of temperature effects on animal cell metabolism in a packed bed process. Biotechnol Bioeng. 2002;77:838-842.
29. Ducommun P, Bolzonella I, Rhiel M, Pugeaud P, von Stockar U, Marison IW. On-line determination of animal cell concentration. Biotechnol Bioeng. 2001;72:515-522.
30. Dowd JE, Jubb A, Kwok KE, Piret JM. Optimization and control of perfusion cultures using a viable cell probe and cell specific perfusion rates. Cytotechnology. 2003;42:35-45.
31. Guan Y, Evans PM, Kemp RB. Specific heat flow rate: an online monitor and potential control variable of specific metabolic rate in animal cell culture that combines microcalorimetry with dielectric spectroscopy. Biotechnol Bioeng. 1998;58:464-477.
32. Guan Y, Kemp R. On-line heat flux measurements improve the culture medium for the growth and productivity of genetically engineered CHO cells. Cytotechnology. 1999;30:107-120.
33. Maskow T, Röllich A, Fetzer I, Yao J, Harms H. Observation of non-linear biomass-capacitance correlations: reasons and implications for bioprocess control. Biosens Bioelectron. 2008; 24:123-128.
34. Matanguihan RM, Konstantinov KB, Yoshida T. Dielectric measurement to monitor the growth and the physiological states of biological cells. Bioprocess Biosyst Eng. 1994;11:213-222.
35. Davey CL, Markx GH, Kell DB. On the dielectric method of monitoring cellular viability. Pure Appl Chem. 1993;65:1921-1926.
36. Noll T, Biselli M. Dielectric spectroscopy in the cultivation of suspended and immobilized hybridoma cells. J Biotechnol. 1998;63:187-198.
37. Cannizzaro C, Gügerli R, Marison I, von Stockar U. On-line biomass monitoring of CHO perfusion culture with scanning dielectric spectroscopy. Biotechnol Bioeng. 2003;84:597-610.
38. Dabros M, Dennewald D, Currie D, Lee M, Todd R, Marison I, von Stockar U. Cole-Cole, linear and multivariate modeling of capacitance data for on-line monitoring of biomass. Bioprocess Biosyst Eng. 2009;32:161-173.
39. Ansorge S, Esteban G, Schmid G. On-line monitoring of infected Sf-9 insect cell cultures by scanning permittivity measurements and comparison with off-line biovolume measurements. Cytotechnology. 2007;55:115-124.
40. Ansorge S, Esteban G, Schmid G. Multifrequency permittivity measurements enable on-line monitoring of changes in intracellular conductivity due to nutrient limitations during batch cultivations of CHO cells. Biotechnol Prog 2010;26:272-283
41. Labeed FH, Coley HM, Hughes MP. Differences in the biophysical properties of membrane and cytoplasm of apoptotic cells revealed using dielectrophoresis. Biochim Biophys Acta. 2006; 1760:922-929.
42. Pethig R, Talary M. Dielectrophoretic detection of membrane morphology changes in Jurkat T-cells undergoing etoposideinduced apoptosis. IET Nanobiotechnol. 2007;1:2-9.
43. Huang Y, Holzel R, Pethig R, Wang X. Differences in the AC electrodynamics of viable and non-viable yeast cells determined through combined dielectrophoresis and electrorotation studies. Phys Med Biol. 1992;37:1499-1517.
44. Singh V. On-line measurement of oxygen uptake in cell culture using the dynamic method. Biotechnol Bioeng. 1996;52:443-448.
45. Markx GH, Davey CL, Kell DB, Morris P. The dielectric permittivity at radio frequencies and the bruggeman probe: novel techniques for the on-line determination of biomass concentrations in plant cell cultures. J Biotechnol. 1991;20:279-290.
46. Geladi P, Kowalski BR. Partial least-squares regression: a tutorial. Anal Chim Acta. 1986;185:1-17.
47. Haaland DM, Thomas EV. Partial least-squares methods for spectral analyses. I. Relation to other quantitative calibration methods and the extraction of qualitative information. Anal Chem. 1988;60:1193-1202.
48. Chalmers JM. Spectroscopy in Process Analysis, 1st ed. Boca Raton, FL: CRC Press; 2000.
49. Freund RJ, Wilson WJ, Sa P. Regression Analysis: Statistical Modeling of a Response Variable, 2nd ed. Burlington, MA: Elsevier; 2006.
50. Winkelmeier P, Glauner B, Lindl T. Quantification of cytotoxicity by cell volume and cell proliferation. Altern Lab Anim. 1993;21:269-280.
51. Kanapitsas A, Vartzeli-Nikaki P, Konsta AA, Visvardis E, Sideris EG. Dielectric properties during apoptosis in peripheral blood cells from chronic lymphocytic leukaemia patients. IEEE Trans Dielectr Electr Insul. 2006;13:1057-1062.
52. Sudsiri J, Wachner D, Gimsa J. On the temperature dependence of the dielectric membrane properties of human red blood cells. Bioelectrochemistry. 2007;70:134-140.
53. Ferris LE, Davey CL, Kell DB. Evidence from its temperature dependence that the b-dielectric dispersion of cell suspensions is not due solely to the charging of a static membrane capacitance. Eur Biophys J. 1990;18:267-276.
54. Zhou X, Burt JPH, Pethig R. Automatic cell electrorotation measurements: studies of the biological effects of low-frequency magnetic fields and of heat shock. Phys Med Biol. 1998;43: 1075-1090.
55. Nebe-von-Caron G, Stephens PJ, Hewitt CJ, Powell JR, Badley RA. Analysis of bacterial function by multi-colour fluorescence flow cytometry and single cell sorting. J Microbiol Methods. 2000;42:97-114.
56. Cook JA, Mitchell JB. Viability measurements in mammalian cell systems. Anal Biochem. 1989;179:1-7.
57. Weichang Z, Hu WS. On-line characterization of a hybridoma cell culture process. Biotechnol Bioeng. 1994;44:170-177.