Escherichia coli dihydrofolate reductase catalyzed proton and hydride transfers: Temporal order and the roles of Asp27 and Tyr100

Proceedings of the National Academy of Sciences of the United States of America

Volumn 111, Issue 51, 2014, Pages 18231-18236

  Liu, C Tony ^a   Francis, Kevin ^b   Layfield, Joshua P ^c   Huang, Xinyi ^a   Hammes Schiffer, Sharon ^c   Kohen, Amnon ^b   Benkovic, Stephen J ^a  

^a PENNSYLVANIA STATE UNIVERSITY   (United States)

^b University of Iowa   (United States)

^c UNIVERSITY OF ILLINOIS AT URBANA CHAMPAIGN   (United States)

Author keywords

Dihydrofolate reductase; Kinetic isotope effect; Mechanism; Synergism

Indexed keywords

ASPARTIC ACID; BACTERIAL ENZYME; DIHYDROFOLATE REDUCTASE; PROTON; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE; TERNARY COMPLEX FACTOR; TETRAHYDROFOLIC ACID; TYROSINE;

ARTICLE; CATALYSIS; ESCHERICHIA COLI; IONIZATION; MOLECULAR MECHANICS; PH; PKA; PROTON TRANSPORT; REACTION ANALYSIS; STATIC ELECTRICITY; TEMPERATURE DEPENDENCE; BIOCATALYSIS; CHEMISTRY; ENZYMOLOGY; METABOLISM; TEMPERATURE;

ASPARTIC ACID; BIOCATALYSIS; ESCHERICHIA COLI; PROTONS; TEMPERATURE; TETRAHYDROFOLATE DEHYDROGENASE; TYROSINE;

EID: 84919934356     PISSN: 00278424     EISSN: 10916490     Source Type: Journal    
DOI: 10.1073/pnas.1415940111     Document Type: Article

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