Considerations for accurate gene expression measurement by reverse transcription quantitative PCR when analysing clinical samples

Analytical and Bioanalytical Chemistry

Volumn 406, Issue 26, 2014, Pages 6471-6483

  Sanders, Rebecca ^a,b   Mason, Deborah J ^b   Foy, Carole A ^a   Huggett, Jim F ^a  

^a Molecular and Cell Biology   (United Kingdom)

^b CARDIFF UNIVERSITY   (United Kingdom)

Author keywords

Normalisation; Reverse transcription quantitative PCR; RNA; Standardisation; Uncertainty

Indexed keywords

GENE EXPRESSION; GENES; POLYMERASE CHAIN REACTION; RNA; STANDARDIZATION;

CLINICAL APPLICATION; CLINICAL SAMPLES; CLINICAL TOOLS; EXPRESSION MEASUREMENTS; GENE EXPRESSION STUDIES; NORMALISATION; REVERSE TRANSCRIPTION QUANTITATIVE-PCR; UNCERTAINTY;

TRANSCRIPTION;

RNA;

ANIMAL; GENE EXPRESSION PROFILING; GENETIC SCREENING; GENETICS; HUMAN; PROCEDURES; REVERSE TRANSCRIPTION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; STANDARDS;

ANIMALS; GENE EXPRESSION PROFILING; GENETIC TESTING; HUMANS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTION; RNA;

EID: 84919860802     PISSN: 16182642     EISSN: 16182650     Source Type: Journal    
DOI: 10.1007/s00216-014-7857-x     Document Type: Review

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