Quality assessment of biobanked nucleic acid extracts for downstream molecular analysis

Biopreservation and Biobanking

Volumn 10, Issue 3, 2012, Pages 266-275

  Wahlberg, Karin ^a   Huggett, Jim ^a   Sanders, Rebecca ^a   Whale, Alexandra S ^a   Bushell, Claire ^a   Elaswarapu, Ramnath ^a   Scott, Daniel J ^a   Foy, Carole A ^a  

^a LGC   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

DNA; NUCLEIC ACID; RNA;

ARTICLE; BLOOD STORAGE; CONTROLLED STUDY; DNA EXTRACTION; ELECTROPHORESIS; FLUORESCENCE; HUMAN; HUMAN TISSUE; MALE; MICROARRAY ANALYSIS; NUCLEIC ACID ANALYSIS; NUCLEIC ACID EXTRACTION; PRIORITY JOURNAL; QUALITY CONTROL; REAL TIME POLYMERASE CHAIN REACTION; RNA EXTRACTION; ULTRAVIOLET SPECTROPHOTOMETRY; BLOOD; BLOOD BANK; ISOLATION AND PURIFICATION; ORGANIZATION AND MANAGEMENT;

BLOOD BANKS; ELECTROPHORESIS; HUMANS; MALE; NUCLEIC ACIDS; QUALITY CONTROL; REAL-TIME POLYMERASE CHAIN REACTION; RNA; SPECTROPHOTOMETRY, ULTRAVIOLET;

EID: 84862885205     PISSN: 19475535     EISSN: 19475543     Source Type: Journal    
DOI: 10.1089/bio.2012.0004     Document Type: Article

References (19)

1. Sambrook J, Russell D. Molecular Cloning, A Laboratory Manual. 3 ed. New York: Cold Spring Harbor; 2001.
2. Scientific T. 260/280 and 260/230 Ratios. T042-Technical Bulletin. 2009.
3. Wilfinger WW, Mackey K, Chomczynski P. Effect of pH and ionic strength on the spectrophotometric assessment of nucleic acid purity. Biotechniques 1997;22:474-476, 478-481.
4. Schroeder A, Mueller O, Stocker S, Salowsky R, Leiber M, Gassmann M, et al. The RIN: An RNA integrity number for assigning integrity values to RNA measurements. BMC Mol Biol 2006;7:3.
5. Fleige S, Pfaffl MW. RNA integrity and the effect on the real-time qRT-PCR performance. Mol Aspects Med 2006;27:126-139.
6. Fleige S, Walf V, Huch S, Prgomet C, Sehm J, Pfaffl MW. Comparison of relative mRNA quantification models and the impact of RNA integrity in quantitative real-time RT-PCR. Biotechnol Lett 2006;28:1601-1613. (Pubitemid 44306128)
7. Bustin SA, Benes V, Garson JA, Hellemans J, Huggett J, Kubista M, et al. The MIQE guidelines: Minimum information for publication of quantitative real-time PCR experiments. Clin Chem 2009;55:611-622.
8. Swango KL, Hudlow WR, Timken MD, Buoncristiani MR. Developmental validation of a multiplex qPCR assay for assessing the quantity and quality of nuclear DNA in forensic samples. Forensic Sci Int 2007;170:35-45. (Pubitemid 46994792)
9. Schrohl AS, Wurtz S, Kohn E, Banks RE, Nielsen HJ, Sweep FC, et al. Banking of biological fluids for studies of disease-associated protein biomarkers. Mol Cell Proteomics 2008;7:2061-2066.
10. Peakman TC, Elliott P. The UK Biobank sample handling and storage validation studies. Int J Epidemiol 2008;37:i2-6.
11. Palmirotta P, Ludovici G, Laura De Marchis M, Savonarola A, Leone B, Spila A, et al. Preanalytical procedures for DNA studies: The experience of the Interinstitutional Multidisciplinary BioBank (BioBIM). Biopreserv Biobank 2011;9:35-46.
12. Lengauer C, Green ED, Cremer T. Fluorescence in situ hybridization of YAC clones after Alu-PCR amplification. Genomics 1992;13:826-828.
13. Applied-Biosystems. Real-time PCR: Understanding Ct. Available from: http://www3.appliedbiosystems.com/cms/groups/mcb-marketing/documents/ generaldocuments/cms-053906.pdf. Last accessed November 1, 2010.
14. Mirsaidov UM, Wang D, Timp W, Timp G. Molecular diagnostics for personal medicine using a nanopore. Wiley Interdiscip Rev Nanomed Nanobiotechnol 2010;2:367-381.
15. Ansorge WJ. Next-generation DNA sequencing techniques. N Biotechnol 2009;25:195-203.
16. Brenan CJ, Roberts D, Hurley J. Nanoliter high-throughput PCR for DNA and RNA profiling. Methods Mol Biol 2009;496: 161-174.
17. Hansson MG. Ethics and biobanks. Br J Cancer 2009;100:8-12.
18. Hansson MG, Maschke KJ. Biobanks: Questioning distinctions. Science 2009;326:797; author reply 799.
19. Carrol ED, Salway F, Pepper SD, Saunders E, Mankhambo LA, Ollier WE, et al. Successful downstream application of the Paxgene Blood RNA system from small blood samples in paediatric patients for quantitative PCR analysis. BMC Immunol 2007;8:20.