Development and assessment of a novel real-time PCR assay for quantitation of hepatitis D virus RNA to study viral kinetics in chronic hepatitis D

Journal of Viral Hepatitis

Volumn 20, Issue 4, 2013, Pages 256-262

  Katsoulidou, A ^a   Manesis, E ^a   Rokka, C ^a   Issaris, C ^a   Pagoni, A ^a   Sypsa, V ^a   Hatzakis, A ^a  

^a UNIVERSITY OF ATHENS MEDICAL SCHOOL   (Greece)

Author keywords

antiviral therapy; HBV DNA; HDV RNA; interferon; quantification; real time PCR

Indexed keywords

ADEFOVIR; ENTECAVIR; HEPATITIS B SURFACE ANTIGEN; LAMIVUDINE; PEGINTERFERON;

ANTIVIRAL THERAPY; ARTICLE; DELTA AGENT HEPATITIS; DRUG BLOOD LEVEL; GENETIC VARIABILITY; HEPATITIS DELTA VIRUS; HUMAN; LIMIT OF DETECTION; LIVER CELL CARCINOMA; MOLECULAR BEACON; NONHUMAN; NUCLEOTIDE SEQUENCE; PATIENT CARE; PRIORITY JOURNAL; REAL TIME POLYMERASE CHAIN REACTION; RNA EXTRACTION; VIRUS LOAD; VIRUS TITRATION;

ADOLESCENT; ADULT; AGED; DRUG MONITORING; FEMALE; HEPATITIS D, CHRONIC; HEPATITIS DELTA VIRUS; HUMANS; MALE; REAL-TIME POLYMERASE CHAIN REACTION; REPRODUCIBILITY OF RESULTS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, VIRAL; SENSITIVITY AND SPECIFICITY; VIRAL LOAD; YOUNG ADULT;

EID: 84875219971     PISSN: 13520504     EISSN: 13652893     Source Type: Journal    
DOI: 10.1111/jvh.12000     Document Type: Article

References (27)

1. Rizzetto M, Hoyer B, Canese MG, Shih JW, Purcell RH, Gerin JL,. Delta Agent: association of delta antigen with hepatitis B surface antigen and RNA in serum of delta-infected chimpanzees. Proc Natl Acad Sci U S A 1980; 77: 6124-6128.
2. Wang KS, Choo QL, Weiner AJ, et al,. Structure, sequence and expression of the hepatitis delta (delta) viral genome. Nature 1986; 323: 508-514.
3. Radjef N, Gordien E, Ivaniushina V, et al,. Molecular phylogenetic analyses indicate a wide and ancient radiation of African hepatitis delta virus, suggesting a deltavirus genus of at least seven major clades. J Virol 2004; 78: 2537-2544.
4. Le Gal F, Gault E, Ripault MP, et al,. Eighth major clade for hepatitis delta virus. Emerg Infect Dis 2006; 12: 1447-1450.
5. Casey JL, Brown TL, Colan EJ, Wignall FS, Gerin JL,. A genotype of hepatitis D virus that occurs in northern South America. Proc Natl Acad Sci U S A 1993; 90: 9016-9020.
6. Hadziyannis SJ,. Decreasing prevalence of hepatitis D virus infection. J Gastroenterol Hepatol 1997; 12: 745-746.
7. Farci P,. Delta hepatitis: an update. J Hepatol 2003; 39 (Suppl 1): S212-S219.
8. Fattovich G, Giustina G, Christensen E, et al,. Influence of hepatitis delta virus infection on morbidity and mortality in compensated cirrhosis type B. The European concerted action on viral hepatitis (Eurohep). Gut 2000; 46: 420-426.
9. Niro GA, Rosina F, Rizzetto M,. Treatment of hepatitis D. J Viral Hepat 2005; 12: 2-9.
10. Yamashiro T, Nagayama K, Enomoto N, et al,. Quantitation of the level of hepatitis delta virus RNA in serum, by real-time polymerase chain reaction-and its possible correlation with the clinical stage of liver disease. J Infect Dis 2004; 189: 1151-1157.
11. Le Gal F, Gordien E, Affolabi D, et al,. Quantification of hepatitis delta virus RNA in serum by consensus real-time PCR indicates different patterns of virological response to interferon therapy in chronically infected patients. J Clin Microbiol 2005; 43: 2363-2369.
12. Pollicino T, Raffa G, Santantonio T, et al,. Replicative and transcriptional activities of hepatitis B virus in patients coinfected with hepatitis B and hepatitis delta viruses. J Virol 2011; 85: 432-439.
13. Schaper M, Rodriguez-Frias F, Jardi R, et al,. Quantitative longitudinal evaluations of hepatitis delta virus RNA and hepatitis B virus DNA shows a dynamic, complex replicative profile in chronic hepatitis B and D. J Hepatol 2010; 52: 658-664.
14. Thompson JD, Higgins DG, Gibson TJ,. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position- specific gap penalties and weight matrix choice. Nucleic Acids Res 1994; 22: 4673-4680.
15. Sokol DL, Zhang X, Lu P, Gewirtz AM,. Real time detection of DNA.RNA hybridization in living cells. Proc Natl Acad Sci U S A 1998; 95: 11538-11543.
16. Paraskevis D, Beloukas A, Haida C, et al,. Development of a new ultra sensitive real-time PCR assay (ultra sensitive RTQ-PCR) for the quantification of HBV- DNA. Virol J 2010; 7: 57.
17. Finney D,. Probit Analysis: Parallel Line Analysis, 3rd edn. Cambridge: Cambridge University Press, 1971.
18. Wedemeyer H,. Re-emerging interest in hepatitis delta: new insights into the dynamic interplay between HBV and HDV. J Hepatol 2010; 52: 627-629.
19. Hofmann J, Frenzel K, Minh BQ, et al,. Quantitative detection and typing of hepatitis D virus in human serum by real-time polymerase chain reaction and melting curve analysis. Diagn Microbiol Infect Dis 2010; 67: 172-179.
20. Mederacke I, Bremer B, Heidrich B, et al,. Establishment of a novel quantitative hepatitis D virus (HDV) RNA assay using the Cobas TaqMan platform to study HDV RNA kinetics. J Clin Microbiol 2010; 48: 2022-2029.
21. Zachou K, Yurdaydin C, Drebber U, et al,. Quantitative HBsAg and HDV-RNA levels in chronic delta hepatitis. Liver Int 2010; 30: 430-437.
22. Ferns RB, Nastouli E, Garson JA,. Quantitation of hepatitis delta virus using a single-step internally controlled real-time RT-qPCR and a full-length genomic RNA calibration standard. J Virol Methods 2011; 179: 189-194.
23. Wedemeyer H,. Hepatitis D revival. Liver Int 2010; 31 (Suppl 1): 140-144.
24. Manesis EK, Schina M, Le Gal F, et al,. Quantitative analysis of hepatitis D virus RNA and hepatitis B surface antigen serum levels in chronic delta hepatitis improves treatment monitoring. Antivir Ther 2007; 12: 381-388.
25. Lau DT, Kleiner DE, Park Y, Di Bisceglie AM, Hoofnagle JH,. Resolution of chronic delta hepatitis after 12 years of interferon alfa therapy. Gastroenterology 1999; 117: 1229-1233.
26. Sakugawa H, Nakasone H, Nakayoshi T, et al,. Hepatitis B virus concentrations in serum determined by sensitive quantitative assays in patients with established chronic hepatitis delta virus infection. J Med Virol 2001; 65: 478-484.
27. Hadziyannis SJ, Sherman M, Lieberman HM, Shafritz DA,. Liver disease activity and hepatitis B virus replication in chronic delta antigen-positive hepatitis B virus carriers. Hepatology 1985; 5: 544-547.