Development of a reverse transcription quantitative real-time PCR-based system for rapid detection and quantitation of hepatitis delta virus in the western Amazon region of Brazil

Journal of Virological Methods

Volumn 197, Issue , 2014, Pages 19-24

  Botelho Souza, Luan Felipo ^a,b,c   dos Santos, Alcione de Oliveira ^a,b,c   Borzacov, Lourdes Maria ^b   Honda, Eduardo Resende ^b   Villalobos Salcedo, Juan Miguel ^a,b,c   Vieira, Deusilene Souza ^a,b,c  

^a INSTITUTO OSWALDO CRUZ   (Brazil)

^b Centro de Pesquisa em Medicina Tropical de RondÔnia   (Brazil)

^c UNIVERSIDADE FEDERAL DE RONDÔNIA   (Brazil)

Author keywords

HDV RNA; Hepatitis delta virus; Hydrolysis probe; Quantitative real time PCR; Viral load

Indexed keywords

COMPLEMENTARY DNA; HEPATITIS B SURFACE ANTIGEN; VIRUS RNA;

ARTICLE; BLOOD SAMPLING; BRAZIL; CONTROLLED STUDY; DNA SEQUENCE; ENZYME LINKED IMMUNOSORBENT ASSAY; HEPATITIS B VIRUS; HEPATITIS C VIRUS; HEPATITIS DELTA VIRUS; HUMAN; IN VITRO STUDY; IN VIVO STUDY; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; POPULATION ABUNDANCE; PRIORITY JOURNAL; PROCESS DEVELOPMENT; QUANTITATIVE ANALYSIS; REAL TIME POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SENSITIVITY ANALYSIS; SENSITIVITY AND SPECIFICITY; SEQUENCE ANALYSIS; VIRUS IDENTIFICATION; VIRUS LOAD; VIRUS PARTICLE;

HEPATITIS B VIRUS; HEPATITIS C VIRUS; HEPATITIS DELTA VIRUS;

HBSAG; HBV; HDV; HDV RNA; HEPATITIS B VIRUS; HEPATITIS B VIRUS SURFACE ANTIGEN; HEPATITIS DELTA VIRUS; HYDROLYSIS PROBE; QUANTITATIVE REAL-TIME PCR; REVERSE-TRANSCRIPTION QUANTITATIVE PCR; RT-QPCR; VIRAL LOAD;

BRAZIL; HEPATITIS D; HEPATITIS DELTA VIRUS; HUMANS; MOLECULAR DIAGNOSTIC TECHNIQUES; REAL-TIME POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; VIRAL LOAD;

EID: 84890839801     PISSN: 01660934     EISSN: 18790984     Source Type: Journal    
DOI: 10.1016/j.jviromet.2013.11.016     Document Type: Article

References (30)

1. Alvarado-Mora M.V., Romano C.M., Gomes-Gouvea M.S., Gutierrez M.F., Carrilho F.J., Pinho J.R. Dynamics of hepatitis D (delta) virus genotype 3 in the Amazon region of South America. Infection, Genetics and Evolution: Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases 2011, 11:1462-1468.
2. Bensabath G., Hadler S.C., Soares M.C., Fields H., Dias L.B., Popper H., Maynard J.E. Hepatitis delta virus infection and Labrea hepatitis. Prevalence and role in fulminant hepatitis in the Amazon Basin. JAMA: The Journal of the American Medical Association 1987, 258:479-483.
3. Bonino F., Heermann K.H., Rizzetto M., Gerlich W.H. Hepatitis delta virus: protein composition of delta antigen and its hepatitis B virus-derived envelope. Journal of Virology 1986, 58:945-950.
4. Bustin S.A. Why the need for qPCR publication guidelinesα - the case for MIQE. Methods 2010, 50:217-226.
5. Buti M., Homs M., Rodriguez-Frias F., Funalleras G., Jardi R., Sauleda S., Tabernero D., Schaper M., Esteban R. Clinical outcome of acute and chronic hepatitis delta over time: a long-term follow-up study. Journal of Viral Hepatitis 2011, 18:434-442.
6. Casey J.L., Bergmann K.F., Brown T.L., Gerin J.L. Determinants of RNA editing in hepatitis delta virus. Progress in Clinical and Biological Research 1993, 382:5-11.
7. Casey J.L., Brown T.L., Colan E.J., Wignall F.S., Gerin J.L. A genotype of hepatitis D virus that occurs in northern South America. Proceedings of the National Academy of Sciences of the United States of America 1993, 90:9016-9020.
8. Castelnau C., Le Gal F., Ripault M.P., Gordien E., Martinot-Peignoux M., Boyer N., Pham B.N., Maylin S., Bedossa P., Deny P., Marcellin P., Gault E. Efficacy of peginterferon alpha-2b in chronic hepatitis delta: relevance of quantitative RT-PCR for follow-up. Hepatology 2006, 44:728-735.
9. Farci P. Treatment of chronic hepatitis D: new advances, old challenges. Hepatology 2006, 44:536-539.
10. Farci P., Niro G.A. Clinical features of hepatitis D. Seminars in Liver Disease 2012, 32:228-236.
11. Ferns R.B., Nastouli E., Garson J.A. Quantitation of hepatitis delta virus using a single-step internally controlled real-time RT-qPCR and a full-length genomic RNA calibration standard. Journal of Virological Methods 2012, 179:189-194.
12. Gomes-Gouvea M.S., Soares M.C., Bensabath G., de Carvalho-Mello I.M., Brito E.M., Souza O.S., Queiroz A.T., Carrilho F.J., Pinho J.R. Hepatitis B virus and hepatitis delta virus genotypes in outbreaks of fulminant hepatitis (Labrea black fever) in the western Brazilian Amazon region. The Journal of General Virology 2009, 90:2638-2643.
13. Hughes S.A., Wedemeyer H., Harrison P.M. Hepatitis delta virus. Lancet 2011, 378:73-85.
14. Jardi R., Buti M., Rodriguez F., Cotrina M., Allende H., Esteban R., Guardia J. Comparative analysis of serological markers of chronic delta infection: HDV-RNA, serum HDAg and anti-HD IgM. Journal of Virological Methods 1994, 50:59-66.
15. Kiesslich D., Crispim M.A., Santos C., Ferreira Fde L., Fraiji N.A., Komninakis S.V., Diaz R.S. Influence of hepatitis B virus (HBV) genotype on the clinical course of disease in patients coinfected with HBV and hepatitis delta virus. The Journal of Infectious Diseases 2009, 199:1608-1611.
16. Kwok S., Higuchi R. Avoiding false positives with PCR. Nature 1989, 339:237-238.
17. Le Gal F., Gault E., Ripault M.P., Serpaggi J., Trinchet J.C., Gordien E., Deny P. Eighth major clade for hepatitis delta virus. Emerging Infectious Diseases 2006, 12:1447-1450.
18. Le Gal F., Gordien E., Affolabi D., Hanslik T., Alloui C., Deny P., Gault E. Quantification of hepatitis delta virus RNA in serum by consensus real-time PCR indicates different patterns of virological response to interferon therapy in chronically infected patients. Journal of Clinical Microbiology 2005, 43:2363-2369.
19. Mederacke I., Bremer B., Heidrich B., Kirschner J., Deterding K., Bock T., Wursthorn K., Manns M.P., Wedemeyer H. Establishment of a novel quantitative hepatitis D virus (HDV) RNA assay using the Cobas TaqMan platform to study HDV RNA kinetics. Journal of Clinical Microbiology 2010, 48:2022-2029.
20. Niro G.A., Smedile A., Ippolito A.M., Ciancio A., Fontana R., Olivero A., Valvano M.R., Abate M.L., Gioffreda D., Caviglia G.P., Rizzetto M., Andriulli A. Outcome of chronic delta hepatitis in Italy: a long-term cohort study. Journal of Hepatology 2010, 53:834-840.
21. Parana R., Kay A., Molinet F., Viana S., Silva L.K., Salcedo J.M., Tavares-Neto J., Lobato C., Rios-Leite M., Matteoni L., D'Oliveira A., Tauil P., Trepo C. HDV genotypes in the western Brazilian Amazon region: a preliminary report. The American Journal of Tropical Medicine and Hygiene 2006, 75:475-479.
22. Romeo R., Del Ninno E., Rumi M., Russo A., Sangiovanni A., de Franchis R., Ronchi G., Colombo M. A 28-year study of the course of hepatitis delta infection: a risk factor for cirrhosis and hepatocellular carcinoma. Gastroenterology 2009, 136:1629-1638.
23. Saracco G., Rosina F., Brunetto M.R., Amoroso P., Caredda F., Farci P., Piantino P., Bonino F., Rizzetto M. Rapidly progressive HBsAg-positive hepatitis in Italy. The role of hepatitis delta virus infection. Journal of Hepatology 1987, 5:274-281.
24. Schaper M., Rodriguez-Frias F., Jardi R., Tabernero D., Homs M., Ruiz G., Quer J., Esteban R., Buti M. Quantitative longitudinal evaluations of hepatitis delta virus RNA and hepatitis B virus DNA shows a dynamic, complex replicative profile in chronic hepatitis B and D. Journal of Hepatology 2010, 52:658-664.
25. Shang D., Hughes S.A., Horner M., Bruce M.J., Dong Y., Carey I., Suddle A.R., Agarwal K., Harrison P.M., Atkins M. Development and validation of an efficient in-house real-time reverse transcription polymerase chain reaction assay for the quantitative detection of serum hepatitis delta virus RNA in a diverse South London population. Journal of Virological Methods 2012, 184:55-62.
26. Smedile A., Farci P., Verme G., Caredda F., Cargnel A., Caporaso N., Dentico P., Trepo C., Opolon P., Gimson A., Vergani D., Williams R., Rizzetto M. Influence of delta infection on severity of hepatitis B. Lancet 1982, 2:945-947.
27. Taylor J.M. Hepatitis delta virus. Virology 2006, 344:71-76.
28. Viana S., Parana R., Moreira R.C., Compri A.P., Macedo V. High prevalence of hepatitis B virus and hepatitis D virus in the western Brazilian Amazon. The American Journal of Tropical Medicine and Hygiene 2005, 73:808-814.
29. Wedemeyer H. Hepatitis D revival. Liver International: Official Journal of the International Association for the Study of the Liver 2011, 31(Suppl. 1):140-144.
30. Yamashiro T., Nagayama K., Enomoto N., Watanabe H., Miyagi T., Nakasone H., Sakugawa H., Watanabe M. Quantitation of the level of hepatitis delta virus RNA in serum, by real-time polymerase chain reaction - and its possible correlation with the clinical stage of liver disease. The Journal of Infectious Diseases 2004, 189:1151-1157.