Validation of high-throughput single cell analysis methodology

Analytical Biochemistry

Volumn 452, Issue 1, 2014, Pages 103-113

  Devonshire, Alison S ^a   Baradez, Marc Olivier ^a   Morley, Gary ^a   Marshall, Damian ^a   Foy, Carole A ^a  

^a LGC   (United Kingdom)

Author keywords

Digital PCR; Gene expression; mRNA; Normalization; RT qPCR; Single cell

Indexed keywords

CELL CULTURE; EFFICIENCY; GENE EXPRESSION; QUALITY CONTROL; STEM CELLS; TRANSCRIPTION; WELL TESTING;

DIGITAL PCR; MRNA; NORMALIZATION; RT-QPCR; SINGLE CELLS;

POLYMERASE CHAIN REACTION;

COMPLEMENTARY DNA; MESSENGER RNA; NERVE CELL ADHESION MOLECULE L1; NEUROGENIN 1; TRANSCRIPTION FACTOR GATA 6; TRANSCRIPTION FACTOR MASH1;

ARTICLE; CALIBRATION; CELL LYSATE; CELL SELECTION; CONTROLLED STUDY; CYTOLYSIS; GENE EXPRESSION; GENE EXPRESSION PROFILING; HUMAN; HUMAN CELL; LIMIT OF DETECTION; MEASUREMENT PRECISION; NEURAL STEM CELL; PRIORITY JOURNAL; PROTEIN EXPRESSION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA ISOLATION; SINGLE CELL ANALYSIS; VALIDATION PROCESS; CELL LINE; GENETICS; NANOTECHNOLOGY; POLYMERASE CHAIN REACTION; PROCEDURES; REVERSE TRANSCRIPTION; VALIDATION STUDY;

CALIBRATION; CELL LINE; LIMIT OF DETECTION; NANOTECHNOLOGY; POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTION; RNA, MESSENGER; SINGLE-CELL ANALYSIS;

EID: 84899440743     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2014.03.001     Document Type: Article

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