Detection of pork adulteration in processed meat by species-specific PCR-QIAxcel procedure based on D-loop and cytb genes

Applied Microbiology and Biotechnology

Volumn 98, Issue 23, 2014, Pages 9805-9816

  Barakat, Hassan ^a   El Garhy, Hoda A S ^a   Moustafa, Mahmoud M A ^a  

^a Benha University   (Egypt)

Author keywords

Adulteration; cytb gene; D loop gene; Halal processed meat; PCR; QIAxcel procedure

Indexed keywords

BEEF; CAPILLARY ELECTROPHORESIS; DNA; GENES; RNA;

ADULTERATION; DETECTION AND QUANTIFICATIONS; PRIMERS SPECIFICITIES; PROCESSED MEAT; QIAXCEL PROCEDURE; QUANTITATIVE DATA; RIBOSOMAL RNA (RRNA); SPECIES SPECIFICS;

POLYMERASE CHAIN REACTION;

DNA; FOOD QUALITY; FOOD SECURITY; GENE EXPRESSION; MUTAGENICITY; POLYMERASE CHAIN REACTION;

CYTOCHROME B; MITOCHONDRIAL DNA; RIBOSOME DNA; RNA 18S;

ANALYSIS; CHEMISTRY; DNA SEQUENCE; EVALUATION STUDY; FOOD CONTAMINATION; FOOD SAFETY; GENETICS; MEAT; MOLECULAR GENETICS; POLYMERASE CHAIN REACTION; PROCEDURES; SENSITIVITY AND SPECIFICITY; TIME;

CYTOCHROMES B; DNA, MITOCHONDRIAL; DNA, RIBOSOMAL; FOOD CONTAMINATION; FOOD SAFETY; MEAT; MOLECULAR SEQUENCE DATA; POLYMERASE CHAIN REACTION; RNA, RIBOSOMAL, 18S; SENSITIVITY AND SPECIFICITY; SEQUENCE ANALYSIS, DNA; TIME FACTORS;

EID: 84911426321     PISSN: 01757598     EISSN: 14320614     Source Type: Journal    
DOI: 10.1007/s00253-014-6084-x     Document Type: Article

References (40)

1. Ali ME, Hashim U, Mustafa S, Che Man YB (2012) Swine-specific PCR-RFLP assay targeting mitochondrial cytochrome B gene for semiquantitative detection of pork in commercial meat products. Food Anal Methods 5:613–623
2. Ali ME, Hashim U, Mustafa S, Che Man YB, Yusop MHM, Kashif M, Dhahi TS, Bari MF, Hakim MA, Latif MA (2011) Nanobiosensor for detection and quantification of DNA sequences in degraded mixed meats. J Nanomater 2011:1–11
3. Armand R, Channon JY, Kintner J, White KA, Miselis KA, Perez RP, Lewis LD (2004) The effects of ethidium bromide induced loss of mitochondrial DNA on mitochondrial phenotype and ultrastructure in a human leukemia T-cell line (MOLT-4 cells). Toxicol Appl Pharmacol 196:68–79
4. Barakat H, Spielvogel A, Hassan M, El-Desouky A, El-Mansy H, Rath F, Meyer V, Stahl U (2010) The antifungal protein AFP from Aspergillus giganteus prevents secondary growth of different Fusarium species on barley. Appl Microbiol Biotechnol 87:617–624
5. Bartlett SE, Davidson WS (1992) FINS (forensically informative nucleotide sequencing): a procedure for identifying the animal origin of biological specimens. Biotechniques 12:408–411
6. Bonne K, Verbeke W (2008) Muslim consumer trust in halal meat status and control in Belgium. Meat Sci 79:113–123
7. Calvo JH, Osta R, Zaragoza P (2002) Quantitative PCR detection of pork in raw and heated ground beef and pâté. J Agric Food Chem 50:5265–5267
8. Calvo JH, Zaragoza P, Osta R (2001) Random amplified polymorphic DNA fingerprints for identification of species in poultry pate. Poult Sci 80:522–524
9. Che Man YB, Mustafa S, Khairil-Mokhtar NF, Nordin R, Sazili AQ (2012) Porcine-specific polymerase chain reaction assay based on mitochondrial D-loop gene for identification of pork in raw meat. Int J Food Prop 15:134–144
10. Chikuni K, Ozutsumi K, Koishikawa T, Kato S (1990) Species identification of cooked meats by DNA hybridization assay. Meat Sci 27:119–128
11. Di Pinto A, Forte VT, Conversano MC, Tantillo GM (2005) Duplex polymerase chain reaction for detection of pork meat in horse meat fresh sausages from Italian retail sources. Food Cont 16:391–394
12. Girish PS, Anjaneyulu ASR, Viswas KN, Shivakumar BM, Anand M, Patel M, Sharma B (2005) Meat species identification by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) of mitochondrial 12S rRNA gene. Meat Sci 70:107–112
13. Graf C, Rüegg-Kuslyte A, Kuhn R (2011) Species determination for meat using PCR-RFLP analysis on the QIAxcel® system. Application Note
14. Hird H, Chisholm A, Sanchez M, Hernandez R, Goodier K, Schneede C, Boltz B, Popping B (2006) Effect of heat and pressure processing on DNA fragmentation and implications for the detection of meat using a real-time polymerase chain reaction. Food Addit Contam 23:645–650
15. Karabasanavar NS, Singh SP, Kumar D, Shebannavar SN (2014) Detection of pork adulteration by highly-specific PCR assay of mitochondrial D-loop. Food Chem 145:530–534
16. Karabasanavar NS, Singh SP, Umapathi V, Girish PS, Shebannavar SN, Kumar D (2011) Authentication of carabeef (water buffalo, Bubalus bubalis) using highly specific polymerase chain reaction. Eur Food Res Technol 233:985–989
17. Kesmen Z, Gulluce A, Sahin F, Yetim H (2009) Identification of meat species by TaqMan-based real-time PCR assay. Meat Sci 82:444–449
18. Kesmen Z, Sahin F, Yetim H (2007) PCR assay for the identification of animal species in cooked sausages. Meat Sci 77:649–653
19. Kumar D, Singh SP, Singh R, Karabasanavar NS (2011) A highly specific PCR assay for identification of goat (Capra hircus) meat. Small Rumin Res 97:76–78
20. Mane BG, Mendiratta SK, Tiwari AK (2013) Pork specific polymerase chain reaction assay for authentication of meat and meat products. J Meat Sci Technol 1:21–27
21. Marois C, Dufour-Gesbert F, Kempf I (2001) Comparison of pulsed-field gel electrophoresis with random amplified polymorphic DNA for typing of Mycoplasma synoviae. Vet Microbiol 79:1–9
22. Matsumoto T, Koshii Y, Sakane K, Murakawa T, Hirayama Y, Yoshida H, Kurokawa M, Tamura Y, Nagai T, Kawase I (2013) A novel approach to automated genotyping of Mycobacterium tuberculosis using a panel of 15 MIRU VNTRs. J Microbiol Methods 93:239–241
23. McMurray CL, Hardy KJ, Hawkey PM (2010) Rapid, automated epidemiological typing of methicillin-resistant Staphylococcus aureus. J Microbiol Methods 80:109–111
24. Melake NA, Shaker GH, Salama MA (2012) Incidence of Helicobacter pylori infection and their clarithromycin-resistant strains in otitis media with effusion regarding phenotypic and genotypic studies. Saudi Pharm J 20:345–353
25. Mercimek-Mahmutoglu S, Sinclair G, van Dooren SJM, Kanhai W, Ashcraft P, Michel OJ, Nelson J, Betsalel OT, Sweetman L, Jakobs C, Salomons GS (2012) Guanidinoacetate methyltransferase deficiency: first steps to newborn screening for a treatable neurometabolic disease. Mol Genet Metab 107:433–437
26. Moghazy EA, El-Shaarawy MOA (2001) Quality attributes of beef burger as affected by using proplies and frozen storage. Egypt J Agric Res 79:1499–1512
27. Moghazy EA, Sharaf SM, El-Seesy TA (2004) Effect of substitutines mutton tailfat with vegetable oil on quality attributes of healthy beef sausage. Egypt J Appl Sci 19:191–205
28. Montiel-Sosa JF, Ruiz-Pesini E, Montoya J, Roncalés P, López-Pérez MJ, Pérez-Martos A (2000) Direct and highly species-specific detection of pork meat and fat in meat products by PCR amplification of mitochondrial DNA. J Agric Food Chem 48:2829–2832
29. Olive PL, Wlodek D, Durand RE, Banáth JP (1992) Factors influencing DNA migration from individual cells subjected to gel electrophoresis. Exp Cell Res 198:259–267
30. Rehbein H, Kress G, Schmidt T (1997) Application of PCR-SSCP to species identification of fishery products. J Sci Food Agric 74:35–41
31. Rojas M, González I, Pavón MA, Pegels N, Lago A, Hernández PE, García T, Martin R (2010) Novel TaqMan real-time polymerase chain reaction assay for verifying the authenticity of meat and commercial meat products from game birds. Food Addit Contam A 27:749–763
32. Skarpeid H, Moe RE, Indahl UG (2001) Detection of mechanically recovered meat and head meat from cattle in ground beef mixtures by multivariate analysis of isoelectric focusing protein profiles. Meat Sci 57:227–234
33. Tanabe S, Hase M, Yano T, Sato M, Fujimura T, Akiyama H (2007a) A real-time quantitative PCR detection method for pork, chicken, beef, mutton, and horseflesh in foods. Biosci Biotechnol Biochem 71:3131–3135
34. Tanabe S, Miyauchi E, Muneshige A, Mio K, Sato C, Sato M (2007b) PCR method of detecting pork in foods for verifying allergen labeling and for identifying hidden pork ingredients in processed foods. Biosci Biotechnol Biochem 71:1663–1667
35. Toorop R, Murch SJ, Ball RO (1997) Methodology and development of prediction equations for the determination of pork substitution in veal. Food Res Int 30:629–636
36. Wissiack R, de la Calle B, Bordin G, Rodriguez AR (2003) Screening test to detect meat adulteration through the determination of hemoglobin by cation exchange chromatography with diode array detection. Meat Sci 64:427–432
37. Xiao M, Kong P, Jin Q, Wang K, Xiao N, Jeoffreys G, James GL, Gilbert GL (2012) Comparison of two capillary gel electrophoresis systems for Clostridium difficile ribotyping, using a panel of ribotype 027 isolates and whole-genome sequences as a reference standard. J Clin Microbiol 50:2755–2760
38. Yang I, Kim Y, Byun J, Park S (2005) Use of multiplex polymerase chain reactions to indicate the accuracy of the annealing temperature of thermal cycling. Anal Biochem 338:192–200
39. Yusop MHM, Mustafa S, Che Man YB, Omar A, Mokhtar N (2012) Detection of raw pork targeting porcine-specific mitochondrial cytochrome B gene by molecular beacon probe real-time polymerase chain reaction. Food Anal Methods 5:422–429
40. Zhang S, Zhao S, Wang Z, Li C (2013) Investigation of parent-of-origin SNPs in 5 imprinted genes for forensic purpose. For Sci Int Genet Suppl Ser 4:e304–e305