The effects of ethidium bromide induced loss of mitochondrial DNA on mitochondrial phenotype and ultrastructure in a human leukemia T-cell line (MOLT-4 cells)

Toxicology and Applied Pharmacology

Volumn 196, Issue 1, 2004, Pages 68-79

  Armand, Ray ^a   Channon, Jacqueline Y ^a   Kintner, Jennifer ^a   White, Kristina A ^a   Miselis, Kristin A ^a   Perez, Raymond P ^a   Lewis, Lionel D ^a  

^a DARTMOUTH HITCHCOCK MEDICAL CENTER   (United States)

Author keywords

Depleted mitochondrial DNA; Ethidium bromide; MOLT 4 0 petite mutants

Indexed keywords

5,5',6,6' TETRACHLORO 1,1',3,3' TETRAETHYLBENZIMIDAZOLYL CARBOCYANINE IODIDE; CITRATE SYNTHASE; CYTOCHROME C OXIDASE; ETHIDIUM BROMIDE; FLUORIDE SODIUM; IODINE DERIVATIVE; LACTIC ACID; MITOCHONDRIAL DNA; UNCLASSIFIED DRUG;

ARTICLE; CELL PROLIFERATION; CELL ULTRASTRUCTURE; CELL VACUOLE; CONTROLLED STUDY; ELECTRON MICROSCOPY; ENZYME ACTIVITY; FLOW CYTOMETRY; HUMAN; HUMAN CELL; INCUBATION TIME; LEUKEMIA CELL; LYMPHOBLASTOID CELL; MEMBRANE POTENTIAL; MITOCHONDRIAL MEMBRANE; NUCLEOTIDE SEQUENCE; OXYGEN CONSUMPTION; PHENOTYPE; POLYMERASE CHAIN REACTION; QUANTITATIVE ASSAY; SOUTHERN BLOTTING; T LYMPHOCYTE; VITAL STAIN; WILD TYPE;

BLOTTING, SOUTHERN; CELL DIVISION; CELL LINE, TUMOR; CELL RESPIRATION; CITRATE (SI)-SYNTHASE; DNA, MITOCHONDRIAL; ELECTRON TRANSPORT COMPLEX IV; ETHIDIUM; HUMANS; LEUKEMIA, T-CELL; MEMBRANE POTENTIALS; MICROSCOPY, ELECTRON; MITOCHONDRIA; MUTATION; OXYGEN;

EID: 1642417871     PISSN: 0041008X     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.taap.2003.12.001     Document Type: Article

References (45)

1. Appleby R.D., Porteous W.K., Hughes G., James A.M., Shannon D., Wei Y.H., Murphy M.P. Quantitation and origin of the mitochondrial membrane potential in human cells lacking mitochondrial DNA. Eur. J. Biochem. 262:1999;108-116.
2. Attardi G., Schatz G. Biogenesis of mitochondria. Annu. Rev. Cell Biol. 4:1998;289-333.
3. Bereiter-Hahn J., Voth M. Dynamics of mitochondria in living cells: shape changes, dislocations, fusion, and fission of mitochondria. Microsc. Res. Tech. 127:1994;198-219.
4. Bradford M. A rapid and sensitive method for quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72:1976;248-254.
5. Chandel N.S., Maltepe E., Goldwasser E., Mathieu C.E., Simon M.C., Schumacker P.T. Mitochondrial reactive oxygen species trigger hypoxia induced transcription. Proc. Natl. Acad. Sci. U.S.A. 95:1998;11715-11720.
6. Chomyn A., Meola G., Bresolin N., Lai S.T., Scarlato G., Attardi G. In vitro genetic transfer of protein synthesis and respiration defects to mitochondrial DNA-less cells with myopathy-patient mitochondria. Mol. Cell. Biol. 11:1991;2236-2244.
7. Corliss D.A., White W.E. Jr. Fluorescence of yeast vitally stained with ethidium bromide and propidium iodide. J. Histochem. Cytochem. 29:1981;45-48.
8. Desjardins P., Frost E., Morais R. Ethidium bromide-induced loss of mitochondrial DNA from primary chicken embryo fibroblasts. Mol. Cell. Biol. 5:1985;1163-1169.
9. Desjardins P., de Muys J.M., Morais R. An established avian fibroblast cell line without mitochondrial DNA. Somatic Cell Mol. Genet. 12:1986;133-139.
10. DiMauro S., Schon E.A. Mitochondrial respiratory-chain diseases. N. Engl. J. Med. 348:2003;2656-2668.
11. Fuller K.M., Duffy C.F., Arriaga E.A. Determination of the cardiolipin content of individual mitochondria by capillary electrophoresis with laser-induced fluorescence detection. Electrophoresis. 23:2002;1571-1576.
12. Gregoire M., Morais R., Quilliam M.A., Gravel D. On auxotrophy for pyrimidines of respiration-deficient chick embryo cells. Eur. J. Biochem. 142:1984;49-55.
13. Hayashi J., Ohta S., Kikuchi A., Takemitsu M., Goto Y., Nonaka I. Introduction of disease-related mitochondrial DNA deletions into HeLa cells lacking mitochondrial DNA results in mitochondrial dysfunction. Proc. Natl. Acad. Sci. U.S.A. 88:1991;10614-10618.
14. Herrnstadt C., Clevenger W., Ghosh S.S., Anderson C., Fahy E., Miller S., Howell N., Davis R.E. A novel mitochondrial DNA-like sequence in the human nuclear genome. Genomics. 60:1999;67-77.
15. James P.E., Grinberg O.Y., Michaels G., Swartz H.M. Intraphagosomal oxygen in stimulated macrophages. J. Cell. Physiol. 163:1995;241-247.
16. James A.M., Wei Y.H., Pang C.Y., Murphy M.P. Altered mitochondrial function in fibroblasts containing MELAS or MERRF mitochondrial DNA mutations. Biochem. J. 318(Pt 2):1996;401-407.
17. Jiang S., Cai J., Wallace D.C., Jones D.P. Cytochrome c-mediated apoptosis in cells lacking mitochondrial DNA. Signaling pathway involving release and caspase 3 activation is conserved. J. Biol. Chem. 274:1999;29905-29911.
18. King M., Attardi G. Human cells lacking mtDNA: repopulation with exogenous mitochondria by complementation. Science. 246:1989;500-503.
19. King M., Attardi G. Isolation of human cell lines lacking mitochondrial DNA. Methods Enzymol. 264:1996;304-313.
20. Lahrichi M., Tarallo P., Houpert Y., Siest G. Influence of glucose and inhibitors of glycolysis on release of total proteins and enzymes from human leukocytes. Clin. Chim. Acta. 79:1977;479-487.
21. Leibowitz R.D. The effect of ethidium bromide on mitochondrial DNA synthesis and mitochondrial DNA structure in HeLa cells. J. Cell Biol. 51:1971;116-122.
22. Lewis L.D., Hamzeh F.M., Lietman P.S. Ultrastructural changes associated with reduced mitochondrial DNA and impaired mitochondrial function in the presence of 2′3′ dideoxycytidine. Antimicrob. Agents Chemother. 36:1992;2061-2065.
23. Marchetti P., Susin S.A., Decaudin D., Gamen S., Castedo M., Hirsch T., Zamzami N., Java J., Senik A., Kroemer G. Apoptosis-associated derangement of mitochondrial function in cells lacking mitochondrial DNA. Cancer Res. 56:1996;2033-2038.
24. 10 putatively acting through the plasma membrane oxidase. Biochem. Mol. Biol. Int. 31:1993;997-1005.
25. Mathur A., Hong Y., Kemp B.K., Barrientos A.A., Erusalimsky J.D. Evaluation of fluorescent dyes for the detection of mitochondrial membrane potential changes in cultured cardiomyocytes. Cardiovasc. Res. 46:2000;126-138.
26. Mileykovskaya E., Dowhan W., Birke R.L., Zheng D., Lutterodt L., Haines T.H. Cardiolipin binds nonyl acridine orange by aggregating the dye at exposed hydrophobic domains on bilayer surfaces. FEBS Lett. 507:2001;187-190.
27. Minowada J., Onuma T., Moore G.E. Rosette-forming human lymphoid cell lines: I. Establishment and evidence for origin of thymus-derived lymphocytes. J. Natl. Cancer Inst. 49:1972;891-895.
28. Morais R. Isolation of avian mitochondrial DNA-less cells. Methods Enzymol. 264:1996;296-304.
29. Morais R., Desjardins P., Turmel C., Zinkewich-Peotti K. Development and characterization of continuous avian cell lines depleted of mitochondrial DNA. In Vitro Cell. Dev. Biol. 24:1988;649-658.
30. Nass M.M. Abnormal DNA patterns in animal mitochondria: ethidium bromide-induced breakdown of closed circular DNA and conditions leading to oligomer accumulation. Proc. Natl. Acad. Sci. U.S.A. 67:1970;1926-1933.
31. Nass M.M. Differential effects of ethidium bromide on mitochondrial and nuclear DNA synthesis in vivo in cultured mammalian cells. Exp. Cell Res. 72:1972;211-222.
32. Petit J.M., Maftah A., Ratinaud M.H., Julien R. Nonyl acridine orange interacts with cardiolipin and allows the quantification of this phospholipid in isolated mitochondria. Eur. J. Biochem. 209:1992;267-273.
33. Ratinaud M.H., Leprat P., Julien R. In situ flow cytometric analysis of nonyl acridine orange-stained mitochondria from splenocytes. Cytometry. 9:1988;206-212.
34. Reers M., Smiley S.T., Mottola-Hartshorn C., Chen A., Lin M., Chen L.B. Mitochondrial membrane potential monitored by JC-1. Methods Enzymol. 260:1995;407-417.
35. Salvioli S., Ardizzoni A., Franceschi C., Cossarizza A. JC-1, but not DiOC6(3) or rhodamine 123, is a reliable fluorescent probe to assess delta psi changes in intact cells: implications for studies on mitochondrial functionality during apoptosis. FEBS Lett. 411:1997;77-82.
36. Skowronek P., Haferkamp O., Rodel G. A fluorescence-microscopic and flow-cytometric study of HeLa cells with an experimentally induced respiratory deficiency. Biochem. Biophys. Res. Commun. 187:1992;991-998.
37. Slonimski P.P., Perrodin G., Croft J.H. Ethidium bromide induced mutation of yeast mitochondria: complete transformation of cells into respiratory deficient non-chromosomal "petites" Biochem. Biophys. Res. Commun. 30:1968;232-239.
38. Tourmen Y., Baris O., Dessen P., Jacques C., Malthiery Y., Reynier P. Structure and chromosomal distribution of human mitochondrial pseudogenes. Genomics. 80:2002;71-77.
39. Trimmer P., Swerdlow R., Parks J., Keeney P., Bennett J. Jr., Miller S., Davis R., Parker W.D. Abnormal mitochondrial morphology in sporadic Parkinson's and Alzheimer's disease cybrid cell lines. Exp. Neurol. 162:2000;37-50.
40. Trounce I.A., Kim Y.L., Jun A.S., Wallace D.C. Assessment of mitochondrial oxidative phosphorylation in patient muscle biopsies, lymphoblasts, and transmitochondrial cell lines. Methods Enzymol. 264:1996;484-509.
41. Wallace K.B., Starkov A.A. Mitochondrial targets of drug toxicity. Annu. Rev. Pharmacol. Toxicol. 40:2000;353-388.
42. Wilson K., Williams J. Chapters 6 and 7 Principles and Techniques of Practical Biochemistry. 5th ed. 2000;312-402 Cambridge Univ. Press, Cambridge, UK.
43. Wittwer C.T., Hermann M.G., Moss A.A., Rasmussen R.P. Continuous fluorescence monitoring of rapid cycle DNA amplification. BioTechniques. 22:1997;130-138.
44. Zhang H., Cooney D.A., Sreenath A., Zhan Q., Agbaria R., Stowe E.E., Fornace A.J. Jr., Johns D.G. Quantitation of mitochondrial DNA in human lymphoblasts by a competitive polymerase chain reaction method: application to the study of inhibitors of mitochondrial DNA content. Mol. Pharmacol. 46:1994;1063-1069.
45. Zylber E., Vesco C., Penman S. Selective inhibition of the synthesis of mitochondria-associated RNA by ethidium bromide. J. Mol. Biol. 44:1969;195-204.