Enhancing tolerance to short-chain alcohols by engineering the Escherichia coli AcrB efflux pump to secrete the non-native substrate n-butanol

ACS Synthetic Biology

Volumn 3, Issue 1, 2014, Pages 30-40

  Fisher, Michael A ^a   Boyarskiy, Sergey ^a,b   Yamada, Masaki R ^a   Kong, Niwen ^a   Bauer, Stefan ^a   Tullman Ercek, Danielle ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b University of California at Berkeley   (United States)

Author keywords

Biofuels; Directed evolution; Efflux pump; Protein engineering; Tolerance; Transporter

Indexed keywords

ACRB PROTEIN; BACTERIAL PROTEIN; BIOFUEL; BUTANOL; CARRIER PROTEIN; ISOBUTANOL; UNCLASSIFIED DRUG; ACRB PROTEIN, E COLI; ESCHERICHIA COLI PROTEIN; MULTIDRUG RESISTANCE PROTEIN;

ALCOHOL TOLERANCE; ARTICLE; BACTERIAL GROWTH; BIOENGINEERING; BIOFUEL PRODUCTION; BIOMASS CONVERSION; ESCHERICHIA COLI; GROWTH RATE; MUTAGENESIS; NONHUMAN; PRIORITY JOURNAL; PROTEIN ENGINEERING; CHEMISTRY; DIRECTED MOLECULAR EVOLUTION; ENZYME SPECIFICITY; GENETICS; METABOLISM; PROTEIN TERTIARY STRUCTURE;

1-BUTANOL; BIOFUELS; BUTANOLS; DIRECTED MOLECULAR EVOLUTION; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; MULTIDRUG RESISTANCE-ASSOCIATED PROTEINS; PROTEIN ENGINEERING; PROTEIN STRUCTURE, TERTIARY; SUBSTRATE SPECIFICITY;

EID: 84896129574     PISSN: None     EISSN: 21615063     Source Type: Journal    
DOI: 10.1021/sb400065q     Document Type: Article

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