Benchmarking organic micropollutants in wastewater, recycled water and drinking water with in vitro bioassays

Environmental Science and Technology

Volumn 48, Issue 3, 2014, Pages 1940-1956

  Escher, Beate I ^a   Allinson, Mayumi ^b,c   Altenburger, Rolf ^d   Bain, Peter A ^e   Balaguer, Patrick ^f   Busch, Wibke ^d   Crago, Jordan ^g   Denslow, Nancy D ^h   Dopp, Elke ⁱ   Hilscherova, Klara ^j   Humpage, Andrew R ^k   Kumar, Anu ^e   Grimaldi, Marina ^f   Jayasinghe, B Sumith ^h   Jarosova, Barbora ^j   Jia, Ai ^l   Makarov, Sergei ^m   Maruya, Keith A ⁿ   Medvedev, Alex ^m   Mehinto, Alvine C ⁿ   Mendez, Jamie E ^o   Poulsen, Anita ^a   Prochazka, Erik ^p   Richard, Jessica ⁱ   Schifferli, Andrea ^q   Schlenk, Daniel ^g   Scholz, Stefan ^d   Shiraishi, Fujio ^c   Snyder, Shane ^l   Su, Guanyong ^r   Tang, Janet Y M ^a   Burg, Bart Van Der ^s   Linden, Sander C Van Der ^s   Werner, Inge ^q   Westerheide, Sandy D ^o   Wong, Chris K C ^t   Yang, Min ^u   Yeung, Bonnie H Y ^t   Zhang, Xiaowei ^r   Leusch, Frederic D L ^p   more..

^a UNIVERSITY OF QUEENSLAND   (Australia)

^b UNIVERSITY OF MELBOURNE   (Australia)

^c NATIONAL INSTITUTE FOR ENVIRONMENTAL STUDIES   (Japan)

^d HELMHOLTZ CENTRE FOR ENVIRONMENTAL RESEARCH UFZ   (Germany)

^e CSIRO   (Australia)

^f INSTITUT DE RECHERCHE EN CANCÉROLOGIE DE MONTPELLIER   (France)

^g UNIVERSITY OF CALIFORNIA   (United States)

^h UNIVERSITY OF FLORIDA   (United States)

ⁱ IWW WATER CENTRE   (Germany)

^j MASARYK UNIVERSITY   (Czech Republic)

^k AUSTRALIAN WATER QUALITY CENTRE   (Australia)

^l UNIVERSITY OF ARIZONA   (United States)

^m Attagene Inc   (United States)

ⁿ SOUTHERN CALIFORNIA COASTAL WATER RESEARCH PROJECT   (United States)

^o UNIVERSITY OF SOUTH FLORIDA   (United States)

^p GRIFFITH UNIVERSITY   (Australia)

^q SWISS FEDERAL INSTITUTE OF AQUATIC SCIENCE AND TECHNOLOGY   (Switzerland)

^r NANJING UNIVERSITY   (China)

^s BIODETECTION SYSTEMS B V   (Netherlands)

^t HONG KONG BAPTIST UNIVERSITY   (Hong Kong)

^u RESEARCH CENTER FOR ECO ENVIRONMENTAL SCIENCES   (China)

more..

Author keywords

[No Author keywords available]

Indexed keywords

CHARCOAL; DRINKING WATER; ESTROGEN; WASTE WATER; WATER; WATER POLLUTANT;

ANALYSIS; ANIMAL; AUSTRALIA; BIOASSAY; EVALUATION STUDY; FILTRATION; IN VITRO STUDY; QUALITY CONTROL; RECYCLING; STANDARDS; TOXICITY TESTING; WASTE WATER; WATER MANAGEMENT; WATER POLLUTANT; WATER QUALITY; ZEBRA FISH;

ANIMALS; AUSTRALIA; BENCHMARKING; BIOLOGICAL ASSAY; CHARCOAL; DRINKING WATER; ESTROGENS; FILTRATION; IN VITRO TECHNIQUES; RECYCLING; TOXICITY TESTS; WASTE WATER; WATER; WATER POLLUTANTS, CHEMICAL; WATER PURIFICATION; WATER QUALITY; ZEBRAFISH;

EID: 84893629531     PISSN: 0013936X     EISSN: 15205851     Source Type: Journal    
DOI: 10.1021/es403899t     Document Type: Article

References (117)

1. NRC. Toxicity Testing in the 21st Century: A Vision and a Strategy. Report in Brief.; National Research Council (NRC): Washington, D.C., 2007.
2. Kavlock, R.; Chandler, K.; Houck, K.; Hunter, S.; Judson, R.; Kleinstreuer, N.; Knudsen, T.; Martin, M.; Padilla, S.; Reif, D.; Richard, A.; Rotroff, D.; Sipes, N.; Dix, D. Update on EPA's ToxCast Program: Providing high throughput decision support tools for chemical risk management Chem. Res. Toxicol. 2012, 25 (7) 1287-1302
3. van der Burg, B.; Kroese, E. D.; Piersma, A. H. Towards a pragmatic alternative testing strategy for the detection of reproductive toxicants Reprod. Toxicol. 2011, 31 (4) 558-561
4. Escher, B.; Leusch, F. Bioanalytical Tools in Water Quality Assessment.; IWA Publishing: London, UK, 2012.
5. Collins, F.; Gray, G. N.; Bucher, J. R. Transforming environmental health protection Science 2008, 319, 906-907
6. Gohlke, J. M.; Portier, C. J. The forest for the trees: A systems approach to human health research Environ. Health Perspect. 2007, 115, 1261-1263
7. Judson, R.; Kavlock, R.; Martin, M.; Reif, D.; Houck, K.; Knudsen, T.; Richard, A.; Tice, R. R.; Whelan, M.; Xia, M.; Huang, R.; Austin, C.; Daston, G.; Hartung, T.; Fowle, J. R., III; Wooge, W.; Tong, W.; Dix, D. Perspectives on validation of high-throughput assays supporting 21st century toxicity testing ALTEX 2013, 30 (1) 51-66
8. Tice, R. R.; Austin, C. P.; Kavlock, R.; Bucher, J. R. Improving the human hazard characterization of chemicals: A Tox21 update Environ. Health Perspect. 2013, 121, 756-765
9. Martin, M. T.; Dix, D. J.; Judson, R. S.; Kavlock, R. J.; Reif, D. M.; Richard, A. M.; Rotroff, D. M.; Romanov, S.; Medvedev, A.; Poltoratskaya, N.; Gambarian, M.; Moeser, M.; Makarov, S. S.; Houck, K. A. Impact of environmental chemicals on key transcription regulators and correlation to toxicity endpoints within EPA's ToxCast program Chem. Res. Toxicol. 2010, 23 (3) 578-590
10. Macova, M.; Toze, S.; Hodgers, L.; Mueller, J. F.; Bartkow, M. E.; Escher, B. I. Bioanalytical tools for the evaluation of organic micropollutants during sewage treatment, water recycling and drinking water generation Water Res. 2011, 45 (14) 4238-4247
11. Escher, B. I.; Lawrence, M.; Macova, M.; Mueller, J. F.; Poussade, Y.; Robillot, C.; Roux, A.; Gernjak, W. Evaluation of contaminant removal of reverse osmosis and advanced oxidation in full-scale operation by combining passive sampling with chemical analysis and bioanalytical tools Environ. Sci. Technol. 2011, 45 (12) 5387-5394
12. Reungoat, J.; Escher, B. I.; Macova, M.; Argaud, F. X.; Gernjak, W.; Keller, J. Ozonation and biological activated carbon filtration of wastewater treatment plant effluents Water Res. 2012, 46 (3) 863-872
13. Reungoat, J.; Escher, B. I.; Macova, M.; Keller, J. Biofiltration of wastewater treatment plant effluent: Effective removal of pharmaceuticals and personal care products and reduction of toxicity Water Res. 2011, 45 (9) 2751-2762
14. Reungoat, J.; Macova, M.; Escher, B. I.; Carswell, S.; Mueller, J. F.; Keller, J. Removal of micropollutants and reduction of biological activity in a full scale reclamation plant using ozonation and activated carbon filtration Water Res. 2010, 44 (2) 625-637
15. Neale, P. A.; Antony, A.; Bartkow, M.; Farre, M.; Heitz, A.; Kristiana, I.; Tang, J. Y. M.; Escher, B. I. Bioanalytical assessment of the formation of disinfection by-products in a drinking water treatment plant Environ. Sci. Technol. 2012, 46 (18) 10317-10325
16. Tang, J. Y. M.; Glenn, E.; Aryal, R.; Gernjak, W.; Escher, B. I. Toxicity characterization of urban stormwater with bioanalytical tools Water Res. 2013, 47 (15) 5594-5606
17. Leusch, F. D. L.; Khan, S. J.; Laingam, S.; Prochazka, E.; Trinh, T.; Froscio, S.; Chapman, H.; Humpage, A., Assessment of the application of bioanalytical tools as surrogate measure of chemical contaminants in recycled water. Water Res. 2014, 49 ((1), 300-315.
18. Tang, J. Y. M.; McCarty, S.; Glenn, E.; Neale, P. A.; Warne, M. S.; Escher, B. I. Mixture effects of organic micropollutants present in water: Towards the development of effect-based water quality trigger values for baseline toxicity Water Res. 2013, 47 (10) 3300-3314
19. Strähle, U.; Scholz, S.; Geisler, R.; Greiner, P.; Hollert, H.; Rastegar, S.; Schumacher, A.; Selderslaghs, I.; Weiss, C.; Witters, H.; Braunbeck, T. Zebrafish embryos as an alternative to animal experiments-A commentary on the definition of the onset of protected life stages in animal welfare regulations Reprod. Toxicol. 2012, 33 (2) 128-132
20. Seo, J. B.; Moon, H. M.; Kim, W. S.; Lee, Y. S.; Jeong, H. W.; Yoo, E. J.; Ham, J. Y.; Kang, H. J.; Park, M. G.; Steffensen, K. R.; Stulnig, T. M.; Gustafsson, J. A.; Dai Park, S.; Kim, J. B. Activated liver X receptors stimulate adipocyte differentiation through induction of peroxisome proliferator-activated receptor gamma expression Mol. Cell. Biol. 2004, 24 (8) 3430-3444
21. Janesick, A.; Blumberg, B. Minireview: PPAR gamma as the target of obesogens J. Steroid Biochem. Mol. Biol. 2011, 127 (1-2) 4-8
22. Neale, P. A.; Escher, B. I., Does dissolved organic carbon cause artefacts in cell-based bioassays? Chemosphere 2013, submitted 25 July 2013.
23. Denison, M. S.; Nagy, S. R. Activation of the aryl hydrocarbon receptor by structurally diverse exogenous and endogenous chemicals Annu. Rev. Pharmacol. Toxicol. 2003, 43, 309-334
24. Odermatt, A.; Gumy, C.; Atanasov, A. G.; Dzyakanchuk, A. A. Disruption of glucocorticoid action by environmental chemicals: Potential mechanisms and relevance J. Steroid Biochem. Mol. Biol. 2006, 102 (1-5) 222-231
25. Omiecinski, C. J.; Heuvel, J. P. V.; Perdew, G. H.; Peters, J. M. Xenobiotic metabolism, disposition, and regulation by receptors: From biochemical phenomenon to predictors of major toxicities Toxicol. Sci. 2011, 120, S49-S75
26. Scarsi, M.; Podvinec, M.; Roth, A.; Hug, H.; Kersten, S.; Albrecht, H.; Schwede, T.; Meyer, U. A.; Rucker, C. Sulfonylureas and glinides exhibit peroxisome proliferator-activated receptor gamma activity: A combined virtual screening and biological assay approach Mol. Pharmacol. 2007, 71 (2) 398-406
27. Huang, R. L.; Xia, M. H.; Cho, M. H.; Sakamuru, S.; Shinn, P.; Houck, K. A.; Dix, D. J.; Judson, R. S.; Witt, K. L.; Kavlock, R. J.; Tice, R. R.; Austin, C. P. Chemical genomics profiling of environmental chemical modulation of human nuclear receptors Environ. Health Perspect. 2011, 119 (8) 1142-1148
28. le Maire, A.; Grimaldi, M.; Roecklin, D.; Dagnino, S.; Vivat-Hannah, V.; Balaguer, P.; Bourguet, W. Activation of RXR-PPAR heterodimers by organotin environmental endocrine disruptors EMBO Rep. 2009, 10 (4) 367-373
29. Riu, A.; Grimaldi, M.; le Maire, A.; Bey, G.; Phillips, K.; Boulahtouf, A.; Perdu, E.; Zalko, D.; Bourguet, W.; Balaguer, P. Peroxisome proliferator-activated receptor gamma is a target for halogenated analogs of bisphenol A Environ. Health Perspect. 2011, 119 (9) 1227-1232
30. Neale, P. A.; Escher, B. I. Co-extracted dissolved organic carbon has a suppressive effect on the acetylcholinesterase inhibition assay Environ. Toxicol. Chem. 2013, 32 (7) 1526-1534
31. Muller, R.; Schreiber, U.; Escher, B. I.; Quayle, P.; Nash, S. M. B.; Mueller, J. F. Rapid exposure assessment of PSII herbicides in surface water using a novel chlorophyll a fluorescence imaging assay Sci. Total Environ. 2008, 401 (1-3) 51-59
32. Stalter, D.; Magdeburg, A.; Wagner, M.; Oehlmann, J. Ozonation and activated carbon treatment of sewage effluents: Removal of endocrine activity and cytotoxicity Water Res. 2011, 45 (3) 1015-1024
33. Van der Linden, S. C.; Heringa, M. B.; Man, H. Y.; Sonneveld, E.; Puijker, L. M.; Brouwer, A.; Van der Burg, B. Detection of multiple hormonal activities in wastewater effluents and surface water, using a panel of steroid receptor CALUX bioassays Environ. Sci. Technol. 2008, 42 (15) 5814-5820
34. Schriks, M.; van der Linden, S. C.; Stoks, P. G. M.; van der Burg, B.; Puijker, L.; de Voogt, P.; Heringa, M. B. Occurrence of glucocorticogenic activity in various surface waters in The Netherlands Chemosphere 2013, 93 (2) 450-454
35. Bruce, G. M.; Corey, L. M.; Mandel, J. H.; Pleus, R. C. Urinary nitrate, thiocyanate, and perchlorate and serum thyroid endpoints based on NHANES 2001 to 2002 J. Occup. Environ. Med. 2013, 55 (1) 64-70
36. Novák, J.; Benísek, M.; Hilscherová, K. Disruption of retinoid transport, metabolism and signaling by environmental pollutants Environ. Int. 2008, 34 (6) 898-913
37. Lefebvre, P.; Benomar, Y.; Staels, B. Retinoid X receptors: Common heterodimerization partners with distinct functions Trends Endocrinol. Metab. 2010, 21 (11) 676-683
38. Tang, J. Y. M.; Glenn, E.; Thoen, H.; Escher, B. I. In vitro bioassay for reactive toxicity towards proteins implemented for water quality monitoring J. Environ. Monit. 2012, 14, 1073-1081
39. Escher, B. I.; Dutt, M.; Maylin, E.; Tang, J. Y. M.; Toze, S.; Wolf, C. R.; Lang, M. Water quality assessment using the AREc32 reporter gene assay indicative of the oxidative stress response pathway J. Environ. Monit. 2012, 14, 2877-2885
40. Clothier, R.; Gomez-Lechon, M. J.; Kinsner-Ovaskainen, A.; Kopp-Schneider, A.; O'Connor, J. E.; Prieto, P.; Stanzel, S. Comparative analysis of eight cytotoxicity assays evaluated within the ACuteTox Project Toxicol. Vitro 2013, 27 (4) 1347-1356
41. Ba, F.; Pang, P. K. T.; Benishin, C. G. The establishment of a reliable cytotoxic system with SK-N-SH neuroblastoma cell culture J. Neurosci. Methods 2003, 123 (1) 11-22
42. Humpage, A. R.; Ledreux, A.; Fanok, S.; Bernard, C.; Briand, J. F.; Eaglesham, G.; Papageorgiou, J.; Nicholson, B.; Steffensen, D. Application of the neuroblastoma assay for paralytic shellfish poisons to neurotoxic freshwater cyanobacteria: Interlaboratory calibration and comparison with other methods of analysis Environ. Toxicol. Chem. 2007, 26 (7) 1512-1519
43. Richardson, S. D.; Plewa, M. J.; Wagner, E. D.; Schoeny, R.; DeMarini, D. M. Occurrence, genotoxicity, and carcinogenicity of regulated and emerging disinfection by-products in drinking water: A review and roadmap for research Mutat. Res. 2007, 636 (1-3) 178-242
44. Escher, B. I.; van Daele, C.; Dutt, M.; Tang, J. Y. M.; Altenburger, R. Most oxidative stress response in water samples comes from unknown chemicals: The need for effect-based water quality trigger values Environ. Sci. Technol. 2013, 47 (13) 7002-7011
45. Kokel, D.; Bryan, J.; Laggner, C.; White, R.; Cheung, C. Y. J.; Mateus, R.; Healey, D.; Kim, S.; Werdich, A. A.; Haggarty, S. J.; MacRae, C. A.; Shoichet, B.; Peterson, R. T. Rapid behavior-based identification of neuroactive small molecules in the zebrafish Nat. Chem. Biol. 2010, 6 (3) 231-237
46. Romanov, S.; Medvedev, A.; Gambarian, M.; Poltoratskaya, N.; Moeser, M.; Medvedeva, L.; Diatchenko, L.; Makarov, S. Homogeneous reporter system enables quantitative functional assessment of multiple transcription factors Nat. Methods 2008, 5 (3) 253-260
47. Lemaire, G.; Mnif, W.; Pascussi, J. M.; Pillon, A.; Rabenoelina, F.; Fenet, H.; Gomez, E.; Casellas, C.; Nicolas, J. C.; Cavailles, V.; Duchesne, M. J.; Balaguer, P. Identification of new human pregnane X receptor ligands among pesticides using a stable reporter cell system Toxicol. Sci. 2006, 91 (2) 501-509
48. Creusot, N.; Kinani, S.; Balaguer, P.; Tapie, N.; LeMenach, K.; Maillot-Marechal, E.; Porcher, J. M.; Budzinski, H.; Ait-Aissa, S. Evaluation of an hPXR reporter gene assay for the detection of aquatic emerging pollutants: Screening of chemicals and application to water samples Anal. Bioanal. Chem. 2010, 396 (2) 569-583
49. Shiraishi, F.; Shirashi, H.; Nishikawa, J.; Nishihara, T.; Morita, M. Development of a simple operational estrogenicity assay system using the yeast two-hybrid system J. Environ. Chem. 2000, 10 (1) 57-64
50. Seimandi, M.; Lemaire, G.; Pillon, A.; Perrin, A.; Carlavan, I.; Voegel, J. J.; Vignon, F.; Nicolas, J. C.; Balaguer, P. Differential responses of PPAR alpha, PPAR delta, and PPAR gamma reporter cell lines to selective PPAR synthetic ligands Anal. Biochem. 2005, 344 (1) 8-15
51. Gijsbers, L.; van Eekelen, H. D. L. M.; de Haan, L. H. J.; Swier, J. M.; Heijink, N. L.; Kloet, S. K.; Man, H. Y.; Bovy, A. G.; Keijer, J.; Aarts, J. M. M. J. G.; van der Burg, B.; Rietjens, I. M. C. M. Induction of peroxisome proliferator-activated receptor gamma (PPAR gamma)-mediated gene expression by tomato (Solanum lycopersicum L.) extracts J. Agric. Food Chem. 2013, 61 (14) 3419-3427
52. Piersma, A. H.; Bosgra, S.; van Duursen, M. B. M.; Hermsen, S. A. B.; Jonker, L. R. A.; Kroese, E. D.; van der Linden, S. C.; Man, H.; Roelofs, M. J. E.; Schulpen, S. H. W.; Schwarz, M.; Uibel, F.; van Vugt-Lussenburg, B. M. A.; Westerhout, J.; Wolterbeek, A. P. M.; van der Burg, B. Evaluation of an alternative in vitro test battery for detecting reproductive toxicants Reprod. Toxicol. 2013, 38, 53-64
53. Gijsbers, L.; Man, H. Y.; Kloet, S. K.; de Haan, L. H.; Keijer, J.; Rietjens, I. M.; van der Burg, B.; Aarts, J. M. Stable reporter cell lines for peroxisome proliferator-activated receptor gamma (PPARgamma)-mediated modulation of gene expression Anal. Biochem. 2011, 414 (1) 77-83
54. Law, A. Y. S.; Wei, X.; Zhang, X. W.; Mak, N. K.; Cheung, K. C.; Wong, M. H.; Giesy, J. P.; Wong, C. K. C. Biological analysis of endocrine-disrupting chemicals in animal meats from the Pearl River Delta, China J. Exposure Sci. Environ. Epidemiol. 2012, 22 (1) 93-100
55. EPA, U.S. Prioritization of the Endocrine Disruptor Screening Program Universe of Chemicals for an Estrogen Receptor Adverse Outcome Pathway Using Computational Toxicology Tools; U.S. Environmental Protection Agency: Washington, DC, 2012.
56. Miller, C. A. A human aryl hydrocarbon receptor signaling pathway constructed in yeast displays additive responses to ligand mixtures Toxicol. Appl. Pharmacol. 1999, 160 (3) 297-303
57. Kamata, R.; Shiraishi, F.; Nakajima, D.; Takigami, H.; Shiraishi, H. Mono-hydroxylated polychlorinated biphenyls are potent aryl hydrocarbon receptor ligands in recombinant yeast cells Toxicol. Vitro 2009, 23 (4) 736-743
58. Nagy, S. R.; Sanborn, J. R.; Hammock, B. D.; Denison, M. S. Development of a green fluorescent protein-based cell bioassay for the rapid and inexpensive detection and characterization of Ah receptor agonists Toxicol. Sci. 2002, 65 (2) 200-210
59. Jarosova, B.; Blaha, L.; Vrana, B.; Randak, T.; Grabic, R.; Giesy, J. P.; Hilscherova, K. Changes in concentrations of hydrophilic organic contaminants and of endocrine-disrupting potential downstream of small communities located adjacent to headwaters Environ. Int. 2012, 45, 22-31
60. Jin, L.; van Mourik, L.; Gaus, C.; Escher, B. Applicability of passive sampling to bioanalytical screening of bioaccumulative chemicals in marine wildlife Environ. Sci. Technol. 2013, 47 (14) 7982-7988
61. Sanderson, J. T.; Aarts, J.; Brouwer, A.; Froese, K. L.; Denison, M. S.; Giesy, J. P. Comparison of Ah receptor-mediated luciferase and ethoxyresorufin-O-deethylase induction in H4IIE cells: Implications for their use as bioanalytical tools for the detection of polyhalogenated aromatic hydrocarbons Toxicol. Appl. Pharmacol. 1996, 137 (2) 316-325
62. Weil, M.; Scholz, S.; Zimmer, M.; Sacher, F.; Duis, K. Gene expression analysis in zebrafish embryos: A potential approach to predict effect concentrations in the fish early life stage test Environ. Toxicol. Chem. 2009, 28 (9) 1970-1978
63. Klüver, N.; Yang, L. X.; Busch, W.; Scheffler, K.; Renner, P.; Strähle, U.; Scholz, S., Transcriptional response of zebrafish embryos exposed to neurotoxic compounds reveals a muscle activity dependent hspb11 expression. PLoS One 2011, 6, (12).
64. Escher, B. I.; Bramaz, N.; Mueller, J. F.; Quayle, P.; Rutishauser, S.; Vermeirssen, E. L. M. Toxic equivalent concentrations (TEQs) for baseline toxicity and specific modes of action as a tool to improve interpretation of ecotoxicity testing of environmental samples J. Environ. Monitor 2008, 10 (5) 612-621
65. Ellman, G. L.; Courtney, K. D.; Andres, V., jr; Featherstone, R. M. A new and rapid colorimetric determination of acetylcholinesterase activity Biochem. Pharmacol. 1961, 7 (2) 88-90 IN1, 91-95
66. Sonneveld, E.; Jansen, H. J.; Riteco, J. A. C.; Brouwer, A.; van der Burg, B. Development of androgen- and estrogen-responsive bioassays, members of a panel of human cell line-based highly selective steroid-responsive bioassays Toxicol. Sci. 2005, 83 (1) 136-148
67. Soto, A. M.; Sonnenschein, C.; Chung, K. L.; Fernandez, M. F.; Olea, N.; Serrano, F. O. The E-SCREEN assay as a tool to identify estrogens: An update on estrogenic environmental pollutants Environ. Health Perspect. 1995, 103 (Suppl. 7) 113-122
68. Routledge, E. J.; Sumpter, J. P. Estrogenic activity of surfactants and some of their degradation products assessed using a recombinant yeast screen Environ. Toxicol. Chem. 1996, 15 (3) 241-248
69. Escher, B. I.; Bramaz, N.; Quayle, P.; Rutishauser, S.; Vermeirssen, E. L. M. Monitoring of the ecotoxicological hazard potential by polar organic micropollutants in sewage treatment plants and surface waters using a mode-of-action based test battery J. Environ. Monit. 2008, 10 (5) 622-631
70. Allinson, M.; Shiraishi, F.; Allinson, G. A comparison of recombinant receptor-reporter gene bioassays and a total estrogen enzyme linked immunosorbent assay for the rapid screening of estrogenic activity in natural and waste waters Bull. Environ. Contam. Toxicol. 2011, 86 (5) 461-464
71. Balaguer, P.; Francois, F.; Comunale, F.; Fenet, H.; Boussioux, A. M.; Pons, M.; Nicolas, J. C.; Casellas, C. Reporter cell lines to study the estrogenic effects of xenoestrogens Sci. Total Environ. 1999, 233 (1-3) 47-56
72. Maggioni, S.; Balaguer, P.; Chiozzotto, C.; Benfenati, E. Screening of endocrine-disrupting phenols, herbicides, steroid estrogens, and estrogenicity in drinking water from the waterworks of 35 Italian cities and from PET-bottled mineral water Environ. Sci. Pollut. Res. 2013, 20 (3) 1649-1660
73. Lemaire, G.; Mnif, W.; Mauvais, P.; Balaguer, P.; Rahmani, R. Activation of alpha- and beta-estrogen receptors by persistent pesticides in reporter cell lines Life Sci. 2006, 79 (12) 1160-1169
74. OECD Test guideline455. Stably Transfected Human Estrogen Receptor-Î' Transcriptional Activation Assay for Detection of Estrogenic Agonist-Activity of Chemicals, TG 305; Environmental Directorate; Organisation for Economic Co-operation and Development: Paris, France, 1984, 2009.
75. Zhang, X. W.; Chang, H.; Wiseman, S.; He, Y. H.; Higley, E.; Jones, P.; Wong, C. K. C.; Al-Khedhairy, A.; Giesy, J. P.; Hecker, M. Bisphenol A disrupts steroidogenesis in human H295R cells Toxicol. Sci. 2011, 121 (2) 320-327
76. He, Y. H.; Wiseman, S. B.; Hecker, M.; Zhang, X. W.; Wang, N.; Perez, L. A.; Jones, P. D.; El-Din, M. G.; Martin, J. W.; Giesy, J. P. Effect of ozonation on the estrogenicity and androgenicity of oil sands process-affected water Environ. Sci. Technol. 2011, 45 (15) 6268-6274
77. Brion, F.; Le Page, Y.; Piccini, B.; Cardoso, O.; Tong, S. K.; Chung, B. C.; Kah, O., Screening estrogenic activities of chemicals or mixtures in vivo using transgenic (cyp19a1b-GFP) zebrafish embryos. PLoS One 2012, 7, (5).
78. van der Burg, B.; Winter, R.; Man, H. Y.; Vangenechten, C.; Berckmans, P.; Weimer, M.; Witters, H.; van der Linden, S. Optimization and prevalidation of the in vitro AR CALUX method to test androgenic and antiandrogenic activity of compounds Reprod. Toxicol. 2010, 30 (1) 18-24
79. Molina-Molina, J. M.; Hillenweck, A.; Jouanin, I.; Zalko, D.; Cravedi, J. P.; Fernandez, M. F.; Pillon, A.; Nicolas, J. C.; Olea, N.; Balaguer, P. Steroid receptor profiling of vinclozolin and its primary metabolites Toxicol. Appl. Pharmacol. 2006, 216 (1) 44-54
80. Delfosse, V.; Grimaldi, M.; Pons, J. L.; Boulahtouf, A.; le Maire, A.; Cavailles, V.; Labesse, G.; Bourguet, W.; Balaguer, P. Structural and mechanistic insights into bisphenols action provide guidelines for risk assessment and discovery of bisphenol A substitutes Proc. Natl. Acad. Sci. U.S.A. 2012, 109 (37) 14930-14935
81. Sohoni, P.; Sumpter, J. Several environmental oestrogens are also anti-androgens J. Endocrinol. 1998, 158 (3) 327-339
82. Thomas, K. V.; Hurst, M. R.; Matthiessen, P.; McHugh, M.; Smith, A.; Waldock, M. J. An assessment of in vitro androgenic activity and the identification of environmental androgens in United Kingdom estuaries Environ. Toxicol. Chem. 2002, 21 (7) 1456-1461
83. Wilson, V. S.; Bobseine, K.; Lambright, C. R.; Gray, L. E. A novel cell line, MDA-kb2, that stably expresses an androgen- and glucocorticoid-responsive reporter for the detection of hormone receptor agonists and antagonists Toxicol. Sci. 2002, 66 (1) 69-81
84. Pavlikova, N.; Blahova, L.; Klan, P.; Bathula, S. R.; Sklenar, V.; Giesy, J. P.; Blaha, L. Enantioselective effects of alpha-hexachlorocyclohexane (HCH) isomers on androgen receptor activity in vitro Chemosphere 2012, 86 (1) 65-69
85. Gutleb, A. C.; Meerts, I. A. T. M.; Bergsma, J. H.; Schriks, M.; Murk, A. J. T-Screen as a tool to identify thyroid hormone receptor active compounds Environ. Toxicol. Pharmacol. 2005, 19 (2) 231-238
86. Fini, J. B.; Riu, A.; Debrauwer, L.; Hillenweck, A.; Le Mevel, S.; Chevolleau, S.; Boulahtouf, A.; Palmier, K.; Balaguer, P.; Cravedi, J. P.; Demeneix, B. A.; Zalko, D. Parallel biotransformation of tetrabromobisphenol a in Xenopus laevis and mammals: Xenopus as a model for endocrine perturbation studies Toxicol. Sci. 2012, 125 (2) 359-367
87. Novak, J.; Benisek, M.; Pachernik, J.; Janosek, J.; Sidlova, T.; Kiviranta, H.; Verta, M.; Giesy, J. P.; Blaha, L.; Hilscherova, K. Interference of contaminated sediment extracts and environmental pollutants with retinoid signaling Environ. Toxicol. Chem. 2007, 26 (8) 1591-1599
88. Kamata, R.; Shiraishi, F.; Nishikawa, J.; Yonemoto, J.; Shiraishi, H. Screening and detection of the in vitro agonistic activity of xenobiotics on the retinoic acid receptor Toxicol. In Vitro 2008, 22 (4) 1050-1061
89. Oda, Y.; Nakamura, S.-I.; Oki, I.; Kato, T.; Shinagawa, H. Evaluation of the new system (umu-test) for the detection of environmental mutagens and carcinogens Mutat. Res. 1985, 147 (5) 219-229
90. ISO13828, Water Quality-Determination of the Genotoxicity of Water and Waste Water Using the Umu-Test; International Organization for Standardization (ISO): Geneva, Switzerland, 1999.
91. Oda, Y.; Yamazaki, H.; Thier, R.; Ketterer, B.; Guengerich, F. P.; Shimada, T. A new Salmonella typhimurium NM5004 strain expressing rat glutathione S-transferase 5-5: Use in detection of genotoxicity of dihaloalkanes using an SOS/umu test system Carcinogenesis 1996, 17 (2) 297-302
92. Quillardet, P.; Huisman, O.; Dari, R.; Hofnung, M. SOS Chromotest, a direct assay of induction of an SOS function in Escherichia coli K-12 to measure genotoxicity Proc. Natl. Acad. Sci. U.S.A. 1982, 79 (19) 5971-5975
93. Skarek, M.; Janosek, J.; Cupr, P.; Kohoutek, J.; Novotna-Rychetska, A.; Holoubek, I. Evaluation of genotoxic and non-genotoxic effects of organic air pollution using in vitro bioassays Environ. Int. 2007, 33 (7) 859-866
94. Ames, B. N.; McCann, J.; Yamasaki, E. Methods for detecting carcinogens and mutagens with the salmonella/mammalian-microsome mutagenicity test Mutat. Res. 1975, 31 (6) 347-363
95. Umbuzeiro, G. D.; Rech, C. M.; Correia, S.; Bergamasco, A. M.; Cardenette, G. H. L.; Fluckiger-Isler, S.; Kamber, M. Comparison of the Salmonella /microsome microsuspension assay with the new microplate fluctuation protocol for testing the mutagenicity of environmental samples Environ. Mol. Mutagen. 2010, 51 (1) 31-38
96. Richard, J. Toxicological in Vitro Evaluation of Organic Micropollutants before and after Oxidative Waste Water Treatment; Universität Duisburg-Essen: Essen, Germany, 2012; http://duepublico.uni-duisburg-essen.de/ servlets/DocumentServlet?id=31124.
97. Reifferscheid, G.; Maes, H. M.; Allner, B.; Badurova, J.; Belkin, S.; Bluhm, K.; Brauer, F.; Bressling, J.; Domeneghetti, S.; Elad, T.; Flückiger-Isler, S.; Grummt, H. J.; Gürtler, R.; Hecht, A.; Heringa, M. B.; Hollert, H.; Huber, S.; Kramer, M.; Magdeburg, A.; Ratte, H. T.; Sauerborn-Klobucar, R.; Sokolowski, A.; Soldan, P.; Smital, T.; Stalter, D.; Venier, P.; Ziemann, C.; Zipperle, J.; Buchinger, S. International round-robin study on the Ames fluctuation test Environ. Mol. Mutagen. 2012, 53 (3) 185-197
98. Fenech, M.; Chang, W. P.; Kirsch-Volders, M.; Holland, N.; Bonassi, S.; Zeiger, E. HUMN project: Detailed description of the scoring criteria for the cytokinesis-block micronucleus assay using isolated human lymphocyte cultures Mutat. Res. 2003, 534 (1-2) 65-75
99. Laingam, S.; Froscio, S. M.; Humpage, A. R. Flow-cytometric analysis of in vitro micronucleus formation: Comparative studies with WIL2-NS human lymphoblastoid and L5178Y mouse lymphoma cell lines Mutat. Res. 2008, 656 (1-2) 19-26
100. Rosenkranz, A. R.; Schmaldienst, S.; Stuhlmeier, K. M.; Chen, W. J.; Knapp, W.; Zlabinger, G. J. A microplate assay for the detection of oxidative products using 2′,7′-dichlorofluorescin-diacetate J. Immunol. Meth. 1992, 156 (1) 39-45
101. Bain, P. A.; Schuller, K. A. Use of fluorogenic probes to differentiate between hydrophilic and lipophilic antioxidant activity in a fish cell line J. Agric. Food Chem. 2012, 60 (3) 699-705
102. Harder, A.; Escher, B. I.; Landini, P.; Tobler, N. B.; Schwarzenbach, R. P. Evaluation of bioanalytical tools for toxicity assessment and mode of toxic action classification of reactive chemicals Environ. Sci. Technol. 2003, 37 (21) 4962-4970
103. Piersma, A. H. Alternative methods for developmental toxicity testing Basic Clin. Pharmacol. Toxicol. 2006, 98 (5) 427-431
104. Wang, X. J.; Hayes, J. D.; Wolf, C. R. Generation of a stable antioxidant response element-driven reporter gene cell line and its use to show redox-dependent activation of Nrf2 by cancer chemotherapeutic agents Cancer Res. 2006, 66 (22) 10983-10994
105. Villeneuve, N. F.; Du, Y.; Wang, X. J.; Sun, Z.; Zhang, D. D. High-throughput screening of chemopreventive compounds targeting Nrf2. In 3rd IEEE International Conference on Nano/Micro Engineered and Molecular Systems; IEEE: New York, NY, 2008; Vols 1-3, pp 1138-1141.
106. Van der Linden, S.; von Bergh, A.; Van Vugt-Lussenburg, B.; Jonker, L.; Brouwer, A.; Teunis, M.; Krul, C.; van der Burg, B.; Development of a panel of high throughput reporter gene assays to 1343 detect genotoxicity and oxidative stress. Mutat. Res. 2013, in press, DOI: 10.1016/j.mrgentox.2013.09.009.
107. Konsoula, R.; Barile, F. A. Correlation of in vitro cytotoxicity with paracellular permeability in Caco-2 cells Toxicol. Vitro 2005, 19 (5) 675-684
108. Mosmann, T. Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays J. Immunol. Meth. 1983, 65 (1-2) 55-63
109. Küster, E.; Altenburger, R. Comparison of cholin- and carboxylesterase enzyme inhibition and visible effects in the zebra fish embryo bioassay under short-term paraoxon-methyl exposure Biomarkers 2006, 11 (4) 341-354
110. Gündel, U.; Kalkhof, S.; Zitzkat, D.; von Bergen, M.; Altenburger, R.; Küster, E. Concentration-response concept in ecotoxicoproteomics: Effects of different phenanthrene concentrations to the zebrafish (Danio rerio) embryo proteome Ecotox. Environ. Saf. 2012, 76, 11-22
111. Knöbel, M.; Busser, F. J. M.; Rico-Rico, A.; Kramer, N. I.; Hermens, J. L. M.; Hafner, C.; Tanneberger, K.; Schirmer, K.; Scholz, S. Predicting adult fish acute lethality with the zebrafish embryo: Relevance of test duration, endpoints, compound properties, and exposure concentration analysis Environ. Sci. Technol. 2012, 46 (17) 9690-9700
112. Manger, R. L.; Leja, L. S.; Lee, S. Y.; Hungerford, J. M.; Hokama, Y.; Dickey, R. W.; Granade, H. R.; Lewis, R.; Yasumoto, T.; Wekell, M. M. Detection of paralytic shellfish poison by rapid cell bioassay: Antagonism of voltage-gated sodium channel active toxins in vitro J. AOAC Int. 1995, 78 (2) 521-527
113. Baqui, A.; Meiller, T. F.; Chon, J. J.; Turng, B. F.; Falkler, W. A. Granulocyte-macrophage colony-stimulating factor amplification of interleukin-1 beta and tumor necrosis factor alpha production in THP-1 human monocytic cells stimulated with lipopolysaccharide of oral microorganisms Clin. Diagn. Lab. Immunol. 1998, 5 (3) 341-347
114. OECD. Test Guideline No. 201. Alga, Growth Inhibition Test; Environmental Directorate, Organisation for Economic Co-operation and Development: Paris, France, 1984.
115. ISO11348-3. Water Quality-Determination of the Inhibitory Effect of Water Samples on the Light Emission of Vibrio Fischeri (Luminescent Bacteria Test); International Organization for Standardization (ISO): Geneva, Switzerland, 1998.
116. GB/T 15441-1995 Water Quality-determination of the Acute Toxicity-Luminescent Bacteria Test; National Standard of China, 1995; ttp://kjs.mep.gov.cn/hjbhbz/bzwb/shjbh/sjcgfffbz/199508/t19950801-67352.htm.
117. Ankley, G. T.; Bennett, R. S.; Erickson, R. J.; Hoff, D. J.; Hornung, M. W.; Johnson, R. D.; Mount, D. R.; Nichols, J. W.; Russom, C. L.; Schmieder, P. K.; Serrrano, J. A.; Tietge, J. E.; Villeneuve, D. L. Adverse outcome pathways: A conceptual framework to support ecotoxicology research and risk assessment Environ. Toxicol. Chem. 2010, 29 (3) 730-741