Ritonavir inhibits HIF-1α-mediated VEGF expression in retinal pigment epithelial cells in vitro

Eye (Basingstoke)

Volumn 28, Issue 1, 2014, Pages 93-101

  Vadlapatla, R K ^a   Vadlapudi, A D ^a   Pal, D ^a   Mukherji, M ^a   Mitra, A K ^a  

^a UNIVERSITY OF MISSOURI KANSAS CITY   (United States)

Author keywords

drug repositioning; hypoxia inducible factor; ocular neovascularization; retinal pigment epithelial cells; ritonavir; vascular endothelial growth factor

Indexed keywords

HYPOXIA INDUCIBLE FACTOR 1ALPHA; MESSENGER RNA; RITONAVIR; VASCULOTROPIN;

ANTIANGIOGENIC ACTIVITY; ARTICLE; CELL HYPOXIA; CELL PROLIFERATION; CONTROLLED STUDY; CULTURE MEDIUM; DRUG CYTOTOXICITY; ENZYME LINKED IMMUNOSORBENT ASSAY; GENE EXPRESSION PROFILING; GENE EXPRESSION REGULATION; HUMAN; HUMAN CELL; IMMUNOBLOTTING; IN VITRO STUDY; PIGMENT CELL; PROTEIN EXPRESSION; PROTEIN SYNTHESIS INHIBITION; REAL TIME POLYMERASE CHAIN REACTION;

ANOXIA; CELL PROLIFERATION; CELLS, CULTURED; DOSE-RESPONSE RELATIONSHIP, DRUG; ENDOTHELIAL CELLS; ENZYME-LINKED IMMUNOSORBENT ASSAY; HIV PROTEASE INHIBITORS; HUMANS; HYPOXIA-INDUCIBLE FACTOR 1, ALPHA SUBUNIT; IMMUNOBLOTTING; REAL-TIME POLYMERASE CHAIN REACTION; RETINAL PIGMENT EPITHELIUM; RITONAVIR; RNA, MESSENGER; VASCULAR ENDOTHELIAL GROWTH FACTOR A;

EID: 84891908840     PISSN: 0950222X     EISSN: 14765454     Source Type: Journal    
DOI: 10.1038/eye.2013.240     Document Type: Article

References (42)

1. Kaur C, Foulds WS, Ling EA. Hypoxia-ischemia and retinal ganglion cell damage. Clin Ophthalmol 2008; 2(4): 879-889
2. Vadlapatla RK, Vadlapudi AD, Mitra AK. Hypoxia-inducible factor-1 (hif-1): A potential target for intervention in ocular neovascular diseases. Curr Drug Targets 2013; 14(8): 919-935
3. Caprara C, Grimm C. From oxygen to erythropoietin: Relevance of hypoxia for retinal development, health and disease. Prog Retin Eye Res 2012; 31(1): 89-119
4. Semenza GL. Hypoxia-inducible factors in physiology and medicine. Cell 2012; 148(3): 399-408
5. Weidemann A, Johnson RS. Biology of HIF-1alpha. Cell Death Differ 2008; 15(4): 621-627
6. Ke Q, Costa M. Hypoxia-inducible factor-1 (HIF-1 Mol Pharmacol 2006; 70(5): 1469-1480
7. Rohwer N, Cramer T. Hypoxia-mediated drug resistance: Novel insights on the functional interaction of HIFs and cell death pathways. Drug Resist Updat 2011; 14(3): 191-201
8. Rankin EB, Giaccia AJ. The role of hypoxia-inducible factors in tumorigenesis. Cell Death Differ 2008; 15(4): 678-685
9. Hewitson KS, Schofield CJ. The HIF pathway as a therapeutic target. Drug Discov Today 2004; 9(16): 704-711
10. Campochiaro PA. Ocular neovascularization. J Mol Med 2013; 91(3): 311-321
11. Ruas JL, Lendahl U, Poellinger L. Modulation of vascular gene expression by hypoxia. Curr Opin Lipidol 2007; 18(5): 508-514
12. Conway EM, Collen D, Carmeliet P. Molecular mechanisms of blood vessel growth. Cardiovasc Res 2001; 49(3): 507-521
13. Andriolo RB, Puga ME. Belfort Junior R, Atallah AN. Bevacizumab for ocular neovascular diseases: A systematic review. Sao Paulo Med J 2009; 127(2): 84-91
14. Kourlas H, Abrams P. Ranibizumab for the treatment of neovascular age-related macular degeneration: A review. Clin Ther 2007; 29(9): 1850-1861
15. Ng EW, Shima DT, Calias P, Cunningham Jr., ET, Guyer DR, Adamis AP. Pegaptanib a targeted anti-VEGF aptamer for ocular vascular disease. Nat Rev Drug Discov 2006; 5(2): 123-132
16. Xu D, Kaiser PK. Intravitreal aflibercept for neovascular age-related macular degeneration. Immunotherapy 2013; 5(2): 121-130
17. Slevin M, Kumar P, Wang Q, Kumar S, Gaffney J, Grau-Olivares M et al. New VEGF antagonists as possible therapeutic agents in vascular disease. Expert Opin Investig Drugs 2008; 17(9): 1301-1314
18. Simo R, Hernandez C. Intravitreous anti-VEGF for diabetic retinopathy: Hopes and fears for a new therapeutic strategy. Diabetologia 2008; 51(9): 1574-1580
19. Srirangam A, Milani M, Mitra R, Guo Z, Rodriguez M, Kathuria H et al. The human immunodeficiency virus protease inhibitor ritonavir inhibits lung cancer cells, in part, by inhibition of survivin. J Thorac Oncol 2011; 6(4): 661-670
20. Kumar S, Bryant CS, Chamala S, Qazi A, Seward S, Pal J et al. Ritonavir blocks AKT signaling, activates apoptosis and inhibits migration and invasion in ovarian cancer cells. Mol Cancer 2009; 8: 26
21. Srirangam A, Mitra R, Wang M, Gorski JC, Badve S, Baldridge L et al. Effects of HIV protease inhibitor ritonavir on Akt-regulated cell proliferation in breast cancer. Clin Cancer Res 2006; 12(6): 1883-1896
22. Maggiorella L, Wen B, Frascogna V, Opolon P, Bourhis J, Deutsch E. Combined radiation sensitizing and antiangiogenic effects of ionizing radiation and the protease inhibitor ritonavir in a head and neck carcinoma model. Anticancer Res 2005; 25(6B): 4357-4362
23. Pati S, Pelser CB, Dufraine J, Bryant JL, Reitz Jr., MS, Weichold FF. Antitumorigenic effects of HIV protease inhibitor ritonavir: Inhibition of Kaposi sarcoma. Blood 2002; 99(10): 3771-3779
24. Ponnaluri VK, Vadlapatla RK, Vavilala DT, Pal D, Mitra AK, Mukherji M. Hypoxia induced expression of histone lysine demethylases: Implications in oxygen-dependent retinal neovascular diseases. Biochem Biophys Res Commun 2011; 415(2): 373-377
25. Xu Y, Zhao H, Zheng Y, Gu Q, Ma J, Xu X. A novel antiangiogenic peptide derived from hepatocyte growth factor inhibits neovascularization in vitro and in vivo. Mol Vis 2010; 16: 1982-1995
26. Vadlapatla RK, Vadlapudi AD, Ponnaluri VK, Pal D, Mukherji M, Mitra AK. Molecular expression and functional activity of efflux and influx transporters in hypoxia induced retinal pigment epithelial cells. Int J Pharm 2013; 454(1): 444-452
27. Pugh CW, Ratcliffe PJ. Regulation of angiogenesis by hypoxia: Role of the HIF system. Nat Med 2003; 9(6): 677-684
28. Das A, McGuire PG. Retinal and choroidal angiogenesis: Pathophysiology and strategies for inhibition. Prog Retin Eye Res 2003; 22(6): 721-748
29. Quaggin SE. Turning a blind eye to anti-VEGF toxicities. J Clin Invest 2012; 122(11): 3849-3851
30. Kurihara T, Westenskow PD, Bravo S, Aguilar E, Friedlander M. Targeted deletion of Vegfa in adult mice induces vision loss. J Clin Invest 2012; 122(11): 4213-4217
31. Oh JH, Oh J, Togloom A, Kim SW, Huh K. Effects of Ginkgo biloba Extract on Cultured Human Retinal Pigment Epithelial Cells under Chemical Hypoxia. Curr Eye Res 2013; 38(10): 1072-1082
32. Chen P, Yin H,Wang Y, Xie L. Inhibition of VEGF expression and corneal neovascularization by shRNA targeting HIF-1alpha in a mouse model of closed eye contact lens wear. Mol Vis 2012; 18: 864-873
33. DeNiro M, Al-Halafi A, Al-Mohanna FH, Alsmadi O, Al-Mohanna FA. Pleiotropic effects of YC-1 selectively inhibit pathological retinal neovascularization and promote physiological revascularization in a mouse model of oxygen-induced retinopathy. Mol Pharmacol 2010; 77(3): 348-367
34. Zhao W, Wang YS, Hui YN, Zhu J, Zhang P, Li X et al. Inhibition of proliferation, migration and tube formation of choroidal microvascular endothelial cells by targeting HIF-1alpha with short hairpin RNA-expressing plasmid DNA in human RPE cells in a coculture system. Graefes Arch Clin Exp Ophthalmol 2008; 246(10): 1413-1422
35. Zhang P, Wang Y, Hui Y, Hu D, Wang H, Zhou J et al. Inhibition of VEGF expression by targeting HIF-1 alpha with small interference RNA in human RPE cells. Ophthalmologica 2007; 221(6): 411-417
36. Wang B, Li H, Yan H, Xiao JG. Genistein inhibited hypoxia-inducible factor-1alpha expression induced by hypoxia and cobalt chloride in human retinal pigment epithelium cells. Methods Find Exp Clin Pharmacol 2005; 27(3): 179-184
37. Forooghian F, Razavi R, Timms L. Hypoxia-inducible factor expression in human RPE cells. Br J Ophthalmol 2007; 91(10): 1406-1410
38. Pore N, Gupta AK, Cerniglia GJ, Jiang Z, Bernhard EJ, Evans SM et al. Nelfinavir down-regulates hypoxiainducible factor 1alpha and VEGF expression and increases tumor oxygenation: Implications for radiotherapy. Cancer Res 2006; 66(18): 9252-9259
39. Jacot JL, Sherris D. Potential Therapeutic Roles for Inhibition of the PI3K/Akt/mTOR Pathway in the Pathophysiology of Diabetic Retinopathy. J Ophthalmol 2011; 2011: 589813
40. Yang XM, Wang YS, Zhang J, Li Y, Xu JF, Zhu J et al. Role of PI3K/Akt and MEK/ERK in mediating hypoxia-induced expression of HIF-1alpha and VEGF in laser-induced rat choroidal neovascularization. Invest Ophthalmol Vis Sci 2009; 50(4): 1873-1879
41. Witmer AN, Vrensen GF, Van Noorden CJ, Schlingemann RO. Vascular endothelial growth factors and angiogenesis in eye disease. Prog Retin Eye Res 2003; 22(1): 1-29
42. Carmeliet P. Mechanisms of angiogenesis and arteriogenesis. Nat Med 2000; 6(4): 389-395