Practical workflow for cryo focused-ion-beam milling of tissues and cells for cryo-TEM tomography

Journal of Structural Biology

Volumn 185, Issue 1, 2014, Pages 32-41

  Hsieh, Chyongere ^a   Schmelzer, Thomas ^b   Kishchenko, Gregory ^a   Wagenknecht, Terence ^a,c   Marko, Michael ^a,c  

^a WADSWORTH CENTER   (United States)

^b TGS Technologies   (United States)

^c STATE UNIVERSITY OF NEW YORK   (United States)

Author keywords

Cryo FIB; Cryo SEM; Cryo TEM; Cryo tomography; Vitreous sections

Indexed keywords

ARTICLE; ARTIFACT; CRYO FOCUSED ION BEAM MILLING; CRYO TRANSMISSION ELECTRON MICROSCOPY; FREEZING; HEAT TRANSFER; MICROTOME; PRIORITY JOURNAL; PROCEDURES CONCERNING CELLS; THICKNESS; TISSUE AND ORGAN PREPARATION AND CULTURE; TRANSMISSION ELECTRON MICROSCOPY; ULTRAMICROTOMY;

CRYO-FIB; CRYO-SEM; CRYO-TEM; CRYO-TOMOGRAPHY; VITREOUS SECTIONS;

CRYOELECTRON MICROSCOPY; ELECTRON MICROSCOPE TOMOGRAPHY; FREEZING; FROZEN SECTIONS; IONS; MICROSCOPY, ELECTRON, TRANSMISSION; MICROTOMY; WORKFLOW;

EID: 84890952715     PISSN: 10478477     EISSN: 10958657     Source Type: Journal    
DOI: 10.1016/j.jsb.2013.10.019     Document Type: Article

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