Focused ion beam milling of vitreous water: Prospects for an alternative to cryo-ultramicrotomy of frozen-hydrated biological samples

Journal of Microscopy

Volumn 222, Issue 1, 2006, Pages 42-47

  Marko, M ^a   Hsieh, C ^a   Moberlychan, W ^c,d   Mannella, C A ^a   Frank, J ^b,c  

^a WADSWORTH CENTER   (United States)

^b HOWARD HUGHES MEDICAL INSTITUTE   (United States)

^c HARVARD UNIVERSITY   (United States)

^d LAWRENCE LIVERMORE NATIONAL LABORATORY   (United States)

Author keywords

Cryo EM; Devitrification; Electron tomography; FIB; Frozen hydrated specimens; Vitreous ice

Indexed keywords

ELECTRIC IMPEDANCE TOMOGRAPHY; FOCUSED ION BEAMS; HEAT TRANSFER; HIGH RESOLUTION TRANSMISSION ELECTRON MICROSCOPY; HYDRATION; IONS; MATERIALS HANDLING; MILLING (MACHINING);

BIOLOGICAL SAMPLES; CRYO-EM; DEVITRIFICATION; ELECTRON TOMOGRAPHY; FOCUSED ION BEAM MILLING; FOCUSED IONS BEAMS; FROZEN-HYDRATED SPECIMEN; THINNINGS; ULTRA-MICROTOMY; VITREOUS ICE;

ICE;

GALLIUM; ICE; WATER;

ARTICLE; FEASIBILITY STUDY; FREEZING; HEAT TRANSFER; HYDRATION; ION THERAPY; PRIORITY JOURNAL; TRANSMISSION ELECTRON MICROSCOPY; ULTRAMICROTOMY; VITRIFICATION;

EID: 33744808536     PISSN: 00222720     EISSN: 13652818     Source Type: Journal    
DOI: 10.1111/j.1365-2818.2006.01567.x     Document Type: Article

References (22)

1. Al-Amoudi, A., Dubochet, J., Gnägi, H., Lüthi, W. Studer, D. 2003 An oscillating cryo-knife reduces cutting-induced deformation of vitreous ultrathin sections. J. Microsc. 212, 26 33.
2. Al-Amoudi, A., Studer, D. Dubochet, J. 2005 Cutting artifacts and cutting process in vitreous sections for cryo-electron microscopy. J. Struct. Biol. 150, 109 121.
3. Dubochet, J., Adrian, M., Chang, J.J., Homo, J.-C., Lepault, J., McDowall, A.W. Schultz, P. 1988 Cryo-electron microscopy of vitrified specimens. Quart. Rev. Biophys. 21, 129 228.
4. Echlin, P. 1992 Low-Temperature Microscopy and Microanalysis. Plenum Press, New York.
5. Egerton, R.F. 1996 Electron Energy-Loss Spectroscopy in the Electron Microscope, 2nd edn. Plenum Press, New York.
6. Frank, J., Wagenknecht, T., McEwen, B.F., Marko, M., Hsieh, C.-E. Mannella, C.A. 2002 Three-dimensional imaging of biological complexity. J. Struct. Biol. 138, 85 91.
7. Gestman, I., Hayles, M., Shi, D., Kumar, G., Giannuzzi, L., Lich, B. Subramaniam, S. 2004 Site-specific 3D imaging of cells and tissues using dualbeam technology. Miscrosc. Microanal. 10 Suppl. 2 1124CD.
8. Hsieh, C.-E., Leith, A., Mannella, C.A., Frank, J. Marko, M. 2006 Towards high-resolution three-dimensional imaging of native mammalian tissue: electron tomography of frozen-hydrated rat liver sections. J. Struct. Biol. 153, 1 13.
9. Hsieh, C.-E., Marko, M., Frank, J. Mannella, C.A. 2002 Electron tomographic analysis of frozen-hydrated tissue sections. J. Struct. Biol. 138, 63 73.
10. Kürner, J., Medalia, O., Linaroudis, A.A. Baumeister, W. 2004 New insights into the structural organization of eukaryotic and prokaryotic cytoskeletons using cryo-electron tomography. Exp. Cell Res. 301, 38 42.
11. Leis, A., Andrees, L., Gruska, M., Al-Amoudi, A., Sartori, A., Dubochet, J. Baumeister, W. 2005 Cryo-electron tomography and fluorescence microscopy of unicellular algae in vitreous cryosections. Microsc. Microanal. 11 Suppl. 2 330CD.
12. Matteson, T.L., Schwartz, S.W., Houge, E.C., Kempshall, B.W. Giannuzzi, L.A. 2002 Electron backscattering diffraction investigation of focused ion beam surfaces. J. Electron. Mat. 31, 33 39.
13. Michel, M., Hillman, T. Müller, M. 1991 Cryosectioning of plant material frozen at high pressure. J. Microsc. 163, 3 18.
14. Mulders, H. 2003 The use of a SEM/FIB dual beam applied to biological samples. G.I.T. Imaging Microsc. 2, 8 10.
15. Nicastro, D., Austin, J., Pierson, J., Gaudette, R., Schwartz, C., Ladinsky, M., Staehelin, L.A. McIntosh, J.R. 2005 Visualizing the macromolecular organization of chloroplast membranes using cryo-electron tomography. Microsc. Microanal. 11 Suppl. 2 150 151.
16. Richter, K. 1994 Cutting artefacts on ultrathin cryosections of biological bulk specimens. Micron, 25, 297 308.
17. Schwartz, C., Nicastro, D., Ladinsky, M.S., Mastronarde, D., O'Toole, E. McIntosh, J.R. 2003 Cryo-electron tomography of frozen-hydrated sections of eukaryotic cells. Microsc. Microanal. 9 Suppl. 2 1166 1167.
18. Shi, S., Sun, S.Q., Andrews, B. Leapman, R.D. 1996 Thickness measurement of hydrated and dehydrated cryosections by EELS. Microsc. Res. Techn. 33, 241 250.
19. Steven, A.C. Aebi, U. 2003 The next ice age: cryo-electron tomography of intact cells. Trends Cell Biol. 13, 107 110.
20. Walther, P. Müller, M. 1999 Biological ultrastructure as revealed by high resolution cryo-SEM of block faces after cryo-sectioning. J. Microsc. 196, 279 287.
21. Zhang, P., Bos, E., Heyman, J., Gnägi, H., Kessel, M., Petere, P.J. Subramaniam, S. 2004 Direct visualization of receptor arrays in frozen-hydrated sections and plunge-frozen specimens of E. coli engineered to overproduce the chemotaxis receptor Tsr. J. Microsc. 216, 76 83.
22. Ziegler, J.F., Biersack, J.P. Littmark, U. 1985 The Stopping and Range of Ions in Solids. Pergamon Press, New York.