Focused ion beam micromachining of eukaryotic cells for cryoelectron tomography

Proceedings of the National Academy of Sciences of the United States of America

Volumn 109, Issue 12, 2012, Pages 4449-4454

  Rigort, Alexander ^a   Bäuerlein, Felix J B ^a   Villa, Elizabeth ^a   Eibauer, Matthias ^a   Laugks, Tim ^a   Baumeister, Wolfgang ^a   Plitzko, Jürgen M ^a  

^a MAX PLANCK INSTITUTE OF BIOCHEMISTRY   (Germany)

Author keywords

cryoFIB; cryoTEM lamella; Electron transparent windows; Frozen hydrated cells; Subtomogram averaging

Indexed keywords

ARTICLE; CELL ISOLATION; CELL STRUCTURE; CONTROLLED STUDY; CRYOELECTRON MICROSCOPY; CYTOPLASM; DICTYOSTELIUM DISCOIDEUM; ELECTRON MICROSCOPY; ELECTRON TOMOGRAPHY; EUKARYOTIC CELL; HUMIDITY; LAMELLAR BODY; NUCLEAR PORE COMPLEX; PRESERVATION; PRIORITY JOURNAL; RADIATION BEAM; RIBOSOME; SCANNING ELECTRON MICROSCOPY; STRUCTURE ANALYSIS; VITRIFICATION;

CRYOELECTRON MICROSCOPY; CRYOULTRAMICROTOMY; DICTYOSTELIUM; ELECTRONS; EQUIPMENT DESIGN; EUKARYOTIC CELLS; FOURIER ANALYSIS; FREEZING; IONS; MACROMOLECULAR SUBSTANCES; MICROSCOPY, ELECTRON, SCANNING; MICROSCOPY, ELECTRON, TRANSMISSION; TEMPERATURE; TOMOGRAPHY, X-RAY COMPUTED; VITRIFICATION;

EUKARYOTA; PROKARYOTA;

EID: 84858629640     PISSN: 00278424     EISSN: 10916490     Source Type: Journal    
DOI: 10.1073/pnas.1201333109     Document Type: Article

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