Cloning, expression and optimized production in a bioreactor of bovine chymosin B in Pichia (Komagataella) pastoris under AOX1 promoter

Protein Expression and Purification

Volumn 92, Issue 2, 2013, Pages 235-244

  Noseda, Diego Gabriel ^a   Recúpero, Matías Nicolás ^a   Blasco, Martín ^a   Ortiz, Gastón Ezequiel ^a   Galvagno, Miguel Angel ^a,b  

^a INSTITUTO DE INVESTIGACIONES BIOTECNOLÓGICAS   (Argentina)

^b UNIVERSIDAD DE BUENOS AIRES   (Argentina)

Author keywords

Bovine chymosin; Fed batch fermentation process; Methanol inducible AOX1 promoter; Optimization; Pichia (Komagataella) pastoris; Purification

Indexed keywords

ALDEHYDE OXIDASE; ASCORBIC ACID; CHYMOSIN; RECOMBINANT PROTEIN; SORBITOL;

ANIMAL; ARTICLE; BIOREACTOR; BOVINE CHYMOSIN; CATTLE; CHEMISTRY; CULTURE MEDIUM; FED-BATCH FERMENTATION PROCESS; FERMENTATION; GENETICS; METABOLISM; METHANOL-INDUCIBLE AOX1 PROMOTER; OPTIMIZATION; PH; PICHIA; PICHIA (KOMAGATAELLA) PASTORIS; PROMOTER REGION; PURIFICATION; TEMPERATURE;

BOVINE CHYMOSIN; FED-BATCH FERMENTATION PROCESS; METHANOL-INDUCIBLE AOX1 PROMOTER; OPTIMIZATION; PICHIA (KOMAGATAELLA) PASTORIS; PURIFICATION;

ALDEHYDE OXIDASE; ANIMALS; ASCORBIC ACID; BIOREACTORS; CATTLE; CHYMOSIN; CULTURE MEDIA; FERMENTATION; HYDROGEN-ION CONCENTRATION; PICHIA; PROMOTER REGIONS, GENETIC; RECOMBINANT PROTEINS; SORBITOL; TEMPERATURE;

EID: 84887071215     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2013.08.018     Document Type: Article

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